Implicações da lipoclasia dermossônica no metabolismo energético e na composição corporal de ratos Wistar saudáveis

Abstract

Implications of dermosonic lipoclasisfor energy metabolism and body composition of healthy Wistar rats Implicações da lipoclasia dermossônica no metabolismo energético e na composição corporal de ratos Wistar saudáveis Gonçalves WLS, Cirqueira JP, Abreu GR, Moysés MR AbstractObjectives: To investigate the implications of Dermosonic lipoclasis (DLC), i.e. lipolysis on subcutaneous white adipose tissue induced by ultrasound, for the energy metabolism and body composition of healthy rats. Methods: Twenty four-month-old male Wistar rats weighting ±380g were randomly divided into two groups: 1) sham control (SC) and 2) low-intensity ultrasound therapy (LIUST). For 10 days, after sedation with 3% vaporized halothane, the animals underwent LIUST (I SATA =3MHz, 1 Wcm -2 , pulsed mode 2:8ms, 30% cycles for 3 minutes) in the infra-abdominal and inguinal regions. Weight measurements, naso-anal length and metabolic parameters (food and water intake and excretion) were monitored during the study. At the end of the treatment, blood samples were collected for biochemical analyses. Retroperitoneal (RET), perirenal (PR), epididymal (EP) and inguinal (ING) adiposity was evaluated. HOMA-IR (homeostasis model assessment) was calculated to estimate insulin resistance. For statistical analyses, the Student t test, ANOVA and the Tukey test were used, and differences were established as p<0.05. Results: Regarding body weight, the SC group (384±9g) did not show any changes, while the treated group (337±2g) showed reductions (p<0.01). This was also seen in relation to food intake: (25±1g) vs. (21±1g). There were also reductions (p<0.05) in the RET, PR and ING fat-pads, obesity index, triglyceride levels and plasma lipoprotein levels. Hyperinsulinemia without changes in glycemia characterized a state of insulin resistance, which was confirmed by HOMA-IR. Conclusions: DLC reduced the food intake and body weight and modified the fat deposition in the RET, PR and ING stores in rats. This changed the lipid profile to produce a significant state of insulin resistance.Key words: ultrasound therapy; dermosonic lipoclasis; adipose tissue; energy metabolism; insulin resistance. ResumoObjetivos: Investigar as implicações da lipoclasia dermossônica (LCD), lipólise do tecido adiposo branco subcutâneo induzido por ultrassom (US), no metabolismo energético e na composição corporal de ratos saudáveis. Métodos: Utilizaram-se 20 ratos Wistar saudáveis, com 4 meses de idade, pesando ±380g, divididos aleatoriamente em 2 grupos: 1) controle-sham (CS), 2) terapia ultrassônica de baixa intensidade (TUSBI). Durante 10 dias, após sedação (halotano-3% vaporizado), os animais eram submetidos à TUSBI (I SATA =3MHz, 1W.cm -2 , modo pulsado 2:8ms, ciclo de 30% por 3 minutos) em região infra-abdominal e inguinal. Medidas de peso, comprimento naso-anal e parâmetros metabólicos (ingestão e excreção) foram controlados durante o estudo. Ao final do tratamento, amostras de sangue foram coletadas para dosagens bioquímicas, e então avaliadas adiposidades retroperitoneal (RET...

Ultrasound Lipoclasia on Subcutaneous Adipose Tissue to Produce Acute Hyperglycemia and Enhance Acute Inflammatory Response in Healthy Female Rats

“…3,14 This study demonstrated that a single application was able to significantly reduce gluteal-femoral WAT, leading to acute alterations in the energetic metabolism homeostasis. The alterations were mainly in glucose uptake by cells and tissues, and there was an increase in the inflammatory response by high levels of CRP and LDH.…”

Efeitos agudos do ultrassom terapêutico de 1-MHz na desobstrução nasal de indivíduos com rinossinusite crônica

“…[17][18][19][20][21][22] LIUST has been shown to modify cellular and humoral signaling factors, especially the bioavailability of nitrous oxide, thereby regulating interstitial permeability and tissue fluid balance; 9,12,13,29 this in turn results in decongestion of the nasopharyngeal mucosa, regression of interstitial edema and of nasal obstruction/congestion in patients with chronic rhinosinusitis. Supporting this assumption, recent reports in the literature have shown strong correlations among nasal mucosa congestion rates, fibrosis, and vascular density in patients with chronic rhinosinusitis.…”

“…Pulsed ultrasound waves act on a cell and/or molecular level by altering membrane permeability, ionic concentration gradients and cell biochemical activity. [14][15][16][17] More recently, ultrasound has been employed as adjuvant therapy in several conditions such as tissue repair, 16 reduction of muscle and joint pain, 17,18 modulation of inflammation, 19,[20][21][22] and reduction of tendon adhesions. [17][18][19] A few studies have demonstrated that LIUST-induced accelerated skin tissue repair results from local modulation of cell mediators/signalers such as nitric oxide, histamine and other cytokines that are involved in inflammation in healing; it also increases the number of fibroblasts and collagen synthesis.…”

“…[17][18][19] A few studies have demonstrated that LIUST-induced accelerated skin tissue repair results from local modulation of cell mediators/signalers such as nitric oxide, histamine and other cytokines that are involved in inflammation in healing; it also increases the number of fibroblasts and collagen synthesis. [16][17][18][19] There are few published clinical studies on LIUST as treatment or adjuvant therapy for chronic rhinosinusitis; [9][10][11][12] it seems plausible that LIUST may reduce nasal block and congestion by its physical and chemical effects on tissues, 13,21,22 reestablishing interstitial mucosal permeability, reducing the viscosity of paranasal sinus secretions and helping eliminate nasal discharges. [13][14][15] Thus, the main purpose of this study was to evaluate the short-term effects of continuous 1 MHz LIUST on nasal block and the symptoms of subjects with chronic rhinosinusitis.…”

Técnicas Não Invasivas Para Redução Da Lipodistrofia Localizada: Evidências Atuais. Ênfase No Tratamento Com Criolipólise.

“…Gonçalves e cols. (2009) concluíram que a terapia ultrassônica induziu a redução da ingestão de comida e o peso corporal e deposição de gordura infra-abdominal de 10 ratos Wistar tratados com ultrassom de 3 MHz, com intensidade de 1 W/cm² por 3 minutos, por 10 dias com intervalo de 2 dias a cada cinco aplicações (22).…”