Implication of MicroRNAs in the Pathophysiology of Cardiac and Vascular Smooth Muscle Cells

Meuth, Valérie Metzinger-Le; M'Baya-Moutoula, Eléonore; Taibi, Fatiha; Massy, Ziad A.; Metzinger, Laurent

doi:10.5772/47770

Cited by 2 publications

(3 citation statements)

References 88 publications

(112 reference statements)

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“…Also, the obtained results were in agreement with those found by Wang et al [45], who saw a significant reduction in miRNA126 levels in ESRD patients. Moreover, Neal et al [44] showed in their studies a visible degradation of the circulating RNAse, confirmed in the ex vivo exosomes study on the epithelium of colon cancer during its incubation with the plasma from CKD patients, in comparison with a control plasma [46]. The dialysis membranes should not allow any passage of larger molecules (30–40 kDa), although the HD process might have influenced the total quantity of the circulating miRNAs.…”

Section: Discussionmentioning

confidence: 94%

Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients

et al. 2017

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BackgroundmicroRNA (miRNA) belongs to the non-coding RNAs family responsible for the regulation of gene expression. Renalase is a protein composed of 342 amino acids, secreted by the kidneys and possibly plays an important role in the regulation of sympathetic tone and blood pressure. The aim of the present study was to investigate plasma renalase concentration, and explore the relationship between miRNA-146a-5p expression and plasma renalase levels in hemodialyzed patients.MethodsThe study population comprised 55 subjects who succumbed to various cardiac events, 27 women and 28 men, aged 65–70 years. The total RNA including miRNA fraction was isolated using QiagenmiRNEasy Serum/Plasma kit according to the manufacturer’s protocol. The isolated miRNAs were analyzed using a quantitative polymerase chain reaction (qRT-PCR) technique. The plasma renalase levels were measured using a commercial ELISA kit.ResultsIn the group of patients with high levels of renalase, higher miRNA-146a expression was found, compared with those with low concentration of renalase. Patients with simultaneous low miRNA-146a expression and high level of renalase were confirmed to deliver a significantly longer survival time compared with other patients.ConclusionsmiRNA-146a and plasma renalase levels were estimated as independent prognostic factors of hemodialyzed patients’ survival time. Patients with low miRNA-146a expression demonstrated a significantly longer survival time in contrast to the patients with a high expression level of miRNA-146a. Moreover, a significantly longer survival time was found in patients with high renalase activity compared with patients with low activity of the enzyme.

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Section: Discussionmentioning

confidence: 94%

Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients

et al. 2017

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“…To confirm this mechanism, an experiment has been conducted on exosomes from colon cancer epithelium, indicating increased degradation when incubated with CKD patient's plasma when compared to control plasma. 112 A study has been conducted to investigate whether the haemodialysis process removes circulatory miRNAs from blood or not.…”

Section: Haemodialysismentioning

confidence: 99%

“…The decreased miRNA may be due to the presence of circulating RNase which degrades the miRNAs. To confirm this mechanism, an experiment has been conducted on exosomes from colon cancer epithelium, indicating increased degradation when incubated with CKD patient's plasma when compared to control plasma 112 …”

Section: Micrornas As Diagnostic Markersmentioning

confidence: 99%