Importance of gas input in aqueous two-phase flotation (ATPF)

Jakob, Lucas; Singer, Jan; Nirschl, Hermann

doi:10.1016/j.ces.2020.116391

Cited by 4 publications

(12 citation statements)

References 36 publications

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“…For the complete apparatus S b is the sum of the individual bubble surface area fluxes in each compartment (S b,i ) It was calculated according to Equation (1), where the gas flow rate of each gassing unit ( . V G,i ), the cross-sectional area of each gassing compartment (A C,i ), and the known Sauter mean diameters (d 3,2,i ) for the different flow rates used (see [22]) are considered.…”

Section: Measurement Techniques and Evaluation Methods Of The Atpf-ex...mentioning

confidence: 99%

“…Due to the different compositions of the phases, there is a density difference of 101 kg/m 3 , leading to the vertical separation of the two phases in the flotation apparatus. The selection of the material system is based on previous work [22,23].…”

Section: Aqueous Two-phase System (Atps)mentioning

confidence: 99%

“…This allows biomolecules to be selectively separated from complex biosuspensions and concentrated in a collecting top phase. Both pharmaceutical and technical molecules [1,[3][4][5][6][7][8][9] and enzymes [10][11][12][13][14][15][16][17][18][19][20][21][22][23] can be separated from plant extracts or fermentations with microalgae, bacteria or fungi. In a review Lee et al [2] summarized the research on ATPF and justified the great potential in direct recovery of biotechnological products by ATPF.…”

Section: Introductionmentioning

confidence: 99%

“…Although ATPF has been well investigated on the material side (product, ATPS, pH), almost no process optimization has been carried out to date. Only recently the important influence of the gas input could be shown [22]: Bubble introduction into the bottom phase can occur via different porous media in ATPF, resulting in different bubble size distributions. The bubble size is crucial for both, enzyme transport and the stability of the aqueous phase boundary between top and bottom phase.…”

Section: Introductionmentioning

confidence: 99%

“…Even if a high amount of gas bubble introduction is targeted, the gas flow rate cannot be chosen arbitrarily high, since this also entails an increase in bubble size. Bubbles that are too large rise very quickly and swirl in the bottom phase as they pass the top phase boundary [22].…”

Section: Introductionmentioning

confidence: 99%

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Optimized Apparatus Design for Continuous Aqueous Two-Phase Flotation (ATPF)

Jakob,

Baust,

Griesinger

et al. 2023

Separations

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Aqueous two-phase flotation (ATPF) is an integrative downstream method for separating and concentrating biomolecules from crude biosuspensions such as fermentation broth. Its continuous operation not only increases throughput, but also makes it recommended for continuous upstreams. In this work, an optimized apparatus design in the form of a horizontal flotation tank is presented. The geometry of the new apparatus for continuous ATPF is optimized using flow simulations so that the two aqueous phases can be pumped uniformly through the flotation tank in co-current or counter-current flow. The implementation of a conductivity measurement enables to characterize the phase mixing effect induced by the rising gas bubbles introduced through three different gassing units. ATPF experiments with the model enzyme phospholipase A2 show that there are favorable combinations of gas flow rates to increase the flotation rate and reduce the back-diffusion at the outlet of the apparatus. This allows high phase exchange rates up to 2/h and hence increases the amount of enzymes that can be recovered per time. Co-current operation of continuous ATPF increased separation efficiency about 14% up to E = 74% compared to counter-current operation.

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Section: Measurement Techniques and Evaluation Methods Of The Atpf-ex...mentioning

confidence: 99%

Section: Aqueous Two-phase System (Atps)mentioning

confidence: 99%