Imprinted proteins as a receptor for detection of zearalenone

Pidenko, Pavel S.; Zhang, Huiyan; Lenain, Pieterjan; Goryacheva, Irina Yu.; Saeger, Sarah De; Beloglazova, Natalia V.

doi:10.1016/j.aca.2018.07.062

Cited by 16 publications

(9 citation statements)

References 32 publications

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“…In this study, biOVA exhibited superior characteristics to Kwa as compared with other biPROs except biMAbs. Under the acidic condition, predominant interactions between protonated Kwa and denatured proteins are hydrophobic interactions rather than electrostatic interactions [ 25 ]. Therefore, these results are accounted for by the hydrophobicity of each protein.…”

Section: Discussionmentioning

confidence: 99%

“…The MAb 2H2 and MAb 1D2 showed potential to be used as probes for bioimprinting. So far, the proteins used for bioimprinting have been limited to BSA and OVA [ 23 , 24 , 25 ]. Since physicochemical properties of the two were not obviously different from other proteins ( Supplementary Table S2 ), other factors may cause changes in configuration suitable for binding of Kwa.…”

Section: Discussionmentioning

confidence: 99%

“…Very few studies have been reported regarding the application of bioimprinting to the quantitative analysis of small molecules. Those that have been reported involved the use of ovalbumin (OVA) for the analysis of aflatoxin B1 [ 23 ] and bovine serum albumin (BSA) for deoxynivalenol and zearalenone [ 24 , 25 ]. The bioimprinting process for small molecules is relatively simple ( Figure 1 ) and includes the following: (i) denaturation of the initial protein under acidic conditions; (ii) the addition of target template molecules to interact with the denatured protein to form a new molecular configuration; (iii) crosslinking of the protein with a bifunctional reagent, such as glutaraldehyde, to stabilize the new conformation; and (iv) dialysis of the stabilized protein to remove the leftover template.…”

Section: Introductionmentioning

confidence: 99%

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Bioimprinting as a Receptor for Detection of Kwakhurin

et al. 2022

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Bioimprinting was performed against ovalbumin (OVA) to confer its binding cavities for kwakhurin (Kwa), an isoflavonoid, produced solely by Pueraria candollei var. mirifica (P. candollei). The characterization of bioimprinted-OVA (biOVA), evaluated by an enzyme-linked immunosorbent assay (ELISA), revealed that it functioned as a specific receptor for Kwa. Using biOVA, two systems, i.e., an indirect competitive ELISA (icELISA) and the even simpler and more rapid competitive enzyme-linked bioimprinted-protein assay (cELBIA), were developed as novel techniques for the quantitative analysis of Kwa in P. candollei and its related products. The two analysis methods were found to have limits of detection (LOD) of 4.0 and 2.5 µg/mL, respectively. The high reliability of the developed icELISA and cELBIA using biOVA was also demonstrated by various validation analyses. Subsequently, bioimprinting was performed using eight other proteins to investigate them as candidate scaffolds for the generation of binding cavities for Kwa. Interestingly, two bioimprinted-IgG monoclonal antibodies (biMAbs) recognized Kwa, but their original binding affinity to hapten was lost. That is, the MAbs obtained a new binding ability to Kwa in exchange for their original binding affinity, raising the possibility that biMAb could be alternatively used as a probe for the quantitative analysis of Kwa as well as biOVA. This is the first report of small molecules recognition by MAbs used as proteins for bioimprinting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioimprinting as a Receptor for Detection of Kwakhurin

et al. 2022

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“…The ingestion of ZEN by animals may cause early sexual maturity and promote the occurrence of DNA adducts and micronuclei by triggering chromosome aberrations. 36,37 In this work, AuNFs modied with an amphiphilic ligand were used to conjugate anti-ZEN monoclonal antibodies via covalent coupling of the amino group of Abs to the carboxyl group of AuNF by a carbodiimide method. The proposed AuNF probe was used as a novel probe (N-AuNF-Abs) of LFA (N-LFA) for the quantitative detection of ZEN in DDGS samples.…”

Section: Introductionmentioning

confidence: 99%

“…The ingestion of ZEN by animals may cause early sexual maturity and promote the occurrence of DNA adducts and micronuclei by triggering chromosome aberrations. 36,37 …”

Section: Introductionmentioning

confidence: 99%