Improved 2,3-butanediol production from corncob acid hydrolysate by fed-batch fermentation using Klebsiella oxytoca

Cheng, Ke-Ke; Liu, Qing; Zhang, Jianan; Li, Jinping; Xu, Jian; Wang, Gehua

doi:10.1016/j.procbio.2009.12.009

Cited by 110 publications

(68 citation statements)

References 25 publications

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“…The use of lignocellulosic compounds for 2,3-BDO has also been reported, e.g. corncob hydrolysates were used in processes with K. oxytoca (Cheng et al, 2010) and K. pneumoniae (Ma et al, 2010a). Glycerol was used for the production of 2,3-BDO as well.…”

Section: 3-butanediolmentioning

confidence: 99%

Use of Glycerol in Biotechnological Applications

Wendisch¹,

Lindner²,

Meiswinkel³

2011

Biodiesel- Quality, Emissions and by-Products

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Section: 3-butanediolmentioning

confidence: 99%

Use of Glycerol in Biotechnological Applications

Wendisch¹,

Lindner²,

Meiswinkel³

2011

Biodiesel- Quality, Emissions and by-Products

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“…Batch and fed-batch fermentations were conducted in a 5-L bioreactor (BaoXingBiao, Shanghai) with an initial broth volume of 3 L. The prepared seed culture was inoculated (5%, v/v) into the optimized SSJ medium with an initial pH of 7.0 (Zhang et al, 2010). The bioreactor was operated at 30 °C with an aeration rate of 0.5 vvm (air volume per culture volume per min).…”

Section: Batch and Fed-batch Fermentationsmentioning

confidence: 99%

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

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“…Recently, efficient production of this chemical has been performed in a geneticallyimproved K. Oxytoca strains (Cheng et al, 2010;. To date, K. pneumonia and K. oxytoca are considered as the most powerful 2,3-butanediol producers as they can consume different substrates, such as cellulose and hemicellulose contained in lignocellulosic biomass (Cheng et al, 2010;Guo et al, 2014).…”

Section: Klebsiella Oxytocamentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Improved 2,3-butanediol production from corncob acid hydrolysate by fed-batch fermentation using Klebsiella oxytoca

Cited by 110 publications

References 25 publications

Use of Glycerol in Biotechnological Applications

Use of Glycerol in Biotechnological Applications

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

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