Improved blastocyst development of single cow OPU-derived presumptive zygotes by group culture with agarose-embedded helper embryos

Deb, Gautam Kumar; Jin, Jong-In; Kwon, Tae-Soon; Choi, Byung Hyun; Bang, Jin-Young; Dey, S. R.; Cho, In Rae; Kong, Il-Keun

doi:10.1186/1477-7827-9-121

Cited by 12 publications

(13 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Senatore et al [158]; Deb et al [159] Somatic cell feeders Group Cryotolerance was positively influenced by the presence of somatic cells and was reflected in survival and hatching rates as well as differential cell counts.…”

Section: Groupmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

View full text Add to dashboard Cite

Despite advantages of in vitro embryo production in many species, widespread use of this technology is limited by generally lower developmental competence of in vitro derived embryos compared to in vivo counterparts. Regardless, in vivo or in vitro gametes and embryos face and must adjust to multiple microenvironments especially at preimplantation stages. Moreover, the embryo has to be able to further adapt to environmental cues in utero to result in the birth of live and healthy offspring. Enormous strides have been made in understanding and meeting stage-specific requirements of preimplantation embryos, but interpretation of the data is made difficult due to the complexity of the wide array of culture systems and the remarkable plasticity of developing embryos that seem able to develop under a variety of conditions. Nevertheless, a primary objective remains meeting, as closely as possible, the preimplantation embryo requirements as provided in vivo. In general, oocytes and embryos develop more satisfactorily when cultured in groups. However, optimization of individual culture of oocytes and embryos is an important goal and area of intensive current research for both animal and human clinical application. Successful culture of individual embryos is of primary importance in order to avoid ovarian superstimulation and the associated physiological and psychological disadvantages for patients. This review emphasizes stage specific shifts in embryo metabolism and requirements and research to optimize in vitro embryo culture conditions and supplementation, with a view to optimizing embryo culture in general, and culture of single embryos in particular.

show abstract

Section: Groupmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…To stimulate follicular recruitment for the OPU procedure, oocyte donors received a single 2 ml GnRH injection, and then 36–48 hours later four FSH injections were given 12 hours apart (10–14 ml total per donor or 120–160 IU each). The OPU procedures were performed 24–36 hours after the last FSH injection as previously described [22] , but with different probes. Probes with frequencies that oscillate between 5.0 and 7.5 MHz were used.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal Study of Reproductive Performance of Female Cattle Produced by Somatic Cell Nuclear Transfer

Polejaeva

Broek²,

Walker³

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

The objective of this study was to determine whether or not reproductive performance in cattle produced by somatic cell nuclear transfer (SCNT) is significantly different from that of their genetic donors. To address this question, we directed two longitudinal studies using different embryo production procedures: (1) superovulation followed by artificial insemination (AI) and embryo collection and (2) ultrasound-guided ovum pick-up followed by in vitro fertilization (OPU-IVF). Collectively, these two studies represent the largest data set available for any species on the reproductive performance of female clones and their genetic donors as measured by their embryo production outcomes in commercial embryo production program. The large-scale study described herein was conducted over a six-year period of time and provides a unique comparison of 96 clones to the 40 corresponding genetic donors. To our knowledge, this is the first longitudinal study on the reproductive performance of cattle clones using OPU-IVF. With nearly 2,000 reproductive procedures performed and more than 9,200 transferable embryos produced, our observations show that the reproductive performance of cattle produced by SCNT is not different compared to their genetic donors for the production of transferable embryos after either AI followed by embryo collection (P = 0.77) or OPU-IVF (P = 0.97). These data are in agreement with previous reports showing that the reproductive capabilities of cloned cattle are equal to that of conventionally produced cattle. In conclusion, results of this longitudinal study once again demonstrate that cloning technology, in combination with superovulation, AI and embryo collection or OPU-IVF, provides a valuable tool for faster dissemination of superior maternal genetics.

show abstract

“…OPU technique has been conducted during the first wave of follicular development, as described (Deb et al, 2011). Hanwoo (body weight, approximately 600 ~ 800 kg) were used for two OPU sessions, 4 days apart.…”

Section: Recovery Of Oocytes By Opu Techniquementioning

confidence: 99%

Effect of OPU Session Periods on the Efficiency of In Vitro Embryo Production in Elite Korean Native Cow

Choi

Song

Park

et al. 2018

J Anim Reprod Biotechnol

View full text Add to dashboard Cite

Up-to-date artificial insemination (AI) using frozen sperm consider as the most widely using technology for improvement of Korean Native Cow (Hanwoo) embryo production. However, it is time consuming, required at least 15~20 years to make more than 6 generations, and their offspring number is limited. To overcome such limitations, superovulation and in vitro fertilization have been developed. For superovulation, the number of produced embryos are not enough for commercialization and donor cows need rest period. This led to use of slaughterhouse ovary for in vitro fertilization, but it is impossible to repeat the collection from the same individual and it only can improve the genetic merits of offspring for one generation. Production of embryos using Ovum PickUp (OPU) technique, where oocytes can be repeatedly collected from living elite donor, might overcome these limitations. In this study, we investigated the possibility of using OPU technique from donors at different age and different session periods for mass-embryo-production. Oocytes were collected from 26 donor cows twice per week, 3-4 months per year, between 2013 and 2016. Results showed that, the average number of embryo produced in first year used donor was significantly higher than that in second year used donor (3.89 ± 2.85 vs 3.29 ± 2.70), however, there was no significant difference between third year used donor (3.51 ± 3.32) and other groups. Taken together, our data showed that repeated using of donor up to three years is possible for in vitro embryo mass-production. Moreover, OPU can be used as suitable embryo producing technique for livestock breed improvement.

show abstract

Improved blastocyst development of single cow OPU-derived presumptive zygotes by group culture with agarose-embedded helper embryos

Cited by 12 publications

References 62 publications

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Longitudinal Study of Reproductive Performance of Female Cattle Produced by Somatic Cell Nuclear Transfer

Effect of OPU Session Periods on the Efficiency of In Vitro Embryo Production in Elite Korean Native Cow

Contact Info

Product

Resources

About