Improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis

Nielsen, Klaus; Kelly, L.; Gall, D.; Nicoletti, P; Kelly, William A.

doi:10.1016/0165-2427(94)05361-u

Cited by 107 publications

(78 citation statements)

References 5 publications

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“…Further, Brucella was supposed to exist at 16% within herd and 60% between-herd prevalence (Ghirotti et al, 1991;Pandey et al, 1999). The diagnostic sensitivity and specificity for the Rose Bengal test (RBT) were assumed to be 90% and 75%, and for competitive ELISA (c-ELISA) 98% and 99%, based on previous validation studies (Nielsen et al, 1995(Nielsen et al, , 1996McGiven et al, 2003). Therefore, at individual animal level, the combined sensitivity and specificity for RBT and c-ELISA in serial (sequential) intepretation were calculated at 88.2% and 99.8%, respectively.…”

Section: Cattlementioning

confidence: 99%

Prevalence of antibodies to Brucella spp. and individual risk Factors of Infection in Traditional Cattle, Goats and Sheep Reared in Livestock–Wildlife Interface Areas of Zambia

Muma

Samui

Siamudaala

et al. 2006

Trop Anim Health Prod

114

102

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Please cite the article as: Muma, J.B., Samui, K.L., Siamudaala, V.M., Oloya, J., Matope, G., Omer, M.K., Munyeme, M., Mubita, C., Skjerve, E., 2006. Prevalence of antibodies to Brucella spp. and individual risk factors of infection in traditional cattle, goats and sheep reared in livestock-wildlife interface areas of Zambia. Tropical Animal Health and Production,. 2 AbstractA cross-sectional study was performed in the livestock-wildlife interface areas of Lochinvar and Blue Lagoon National Parks and the non-interface area of Kazungula to determine the prevalence of antibodies to Brucella spp. in domestic ruminants and identify individual animal risk factors of infection. Atotal of 1245 cattle from 124 herds and 280 goats and sheep from 29 flocks were tested sequentially for Brucella antibodies using the Rose Bengal test (RBT) and competitive ELISA. In cattle, individual seroprevalence ranged from 14.1% to 28.1%, while herd sero-prevalence ranged from 46.2% to 74.0% in the three study areas. No goat or sheep tested positive for Brucella antibodies. Three types of cattle grazing strategies were encountered: locally grazed herds (LGH), transhumantly grazed herds (TGH) and river flood plain grazed herds (FGH). Brucella seroprevalence was seen to vary according to area and grazing strategy: Lochinvar and transhumant grazed herds recorded the highest figures, respectively. Age, sex and history of abortion were found to have independent effects on individual seroprevalence. This study establishes that brucellosis is endemic in domestic animals in the livestock-wildlife interface areas of Blue Lagoon and Lochinvar national parks and the disease is also present in Kazungula. We observed that type of grazing strategy had significant impact on cattle Brucella seroprevalence and that transhumant herds were at high risk of being infected.

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Section: Cattlementioning

confidence: 99%

Prevalence of antibodies to Brucella spp. and individual risk Factors of Infection in Traditional Cattle, Goats and Sheep Reared in Livestock–Wildlife Interface Areas of Zambia

Muma

Samui

Siamudaala

et al. 2006

Trop Anim Health Prod

114

102

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“…The threshold for detection was established using bovine sera and was set at Ն30% inhibition of monoclonal antibody binding (Nielsen et al, 1995). Sera from all seals met or exceeded that value.…”

mentioning

confidence: 99%

Brucellosis in Ringed Seals and Harp Seals from Canada

Forbes

Nielsen

Measures

et al. 2000

Journal of Wildlife Diseases

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A novel Brucella sp. was isolated from lymph nodes of four ringed seals (Phoca hispida) collected near Pangnirtung (Baffin Island, Canada) in January and February 1995 and in one harp seal (Phoca groenlandica) collected near the Magdalen Islands (Gulf of St. Lawrence, Canada) in March 1996. Bacteriological characteristics were the same for all five isolates. The colonies were typical of Brucella spp., but took 2 to 5 days longer than the traditional species to appear on primary isolation media. Biotyping results did not match any of the known biovars of Brucella, but were similar to isolates of the genus Brucella previously reported from marine mammals inhabiting other areas of the northern hemisphere. This is the first confirmed report of brucellosis in marine mammals from Canada, and the first report of this organism in ringed and harp seals.

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“…However, test statistics, per the package insert of commercially available assays, are compared against other ELISA assays and not culture. In the peer-reviewed literature, the cELISA, with a cutoff of 1:10 has a sensitivity of 100% and a specificity of 99.7% [120,121]. …”

Section: Diagnosticsmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

152

129

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Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

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Improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis

Cited by 107 publications

References 5 publications

Prevalence of antibodies to Brucella spp. and individual risk Factors of Infection in Traditional Cattle, Goats and Sheep Reared in Livestock–Wildlife Interface Areas of Zambia

Prevalence of antibodies to Brucella spp. and individual risk Factors of Infection in Traditional Cattle, Goats and Sheep Reared in Livestock–Wildlife Interface Areas of Zambia

Brucellosis in Ringed Seals and Harp Seals from Canada

Comparisons of brucellosis between human and veterinary medicine

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