Improved cytotoxicity and preserved level of cell death induced in colon cancer cells by doxorubicin after its conjugation with iron-oxide magnetic nanoparticles

Augustin, Ewa; Czubek, Bartłomiej; Nowicka, Anna M.; Kowalczyk, Agata; Stojek, Zbigniew; Mazerska, Zofia

doi:10.1016/j.tiv.2016.02.009

Cited by 37 publications

(14 citation statements)

References 33 publications

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“…On the other hand, similarly to the electrostatic formulation, Prussian blue staining allowed us to detect high retention rates of covalent formulations inside the cells, as well as, different alterations in cellular morphology depending on the type of covalent samples, mainly apoptosis and mitotic catastrophe. According to our results, several studies have reported an apoptotic response after incubation with DOX covalently bonded to different types of iron oxide magnetic nanoparticle [38,39]. However, there are very few studies related to mitotic catastrophe for DOX-loaded nanoparticles [40].…”

Section: Discussionsupporting

confidence: 61%

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Lázaro-Carrillo

Calero

Aires

et al. 2020

Cancers

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Nanotechnology-based approaches hold substantial potential to avoid chemoresistance and minimize side effects. In this work, we have used biocompatible iron oxide magnetic nanoparticles (MNPs) called MF66 and functionalized with the antineoplastic drug doxorubicin (DOX) against MDA-MB-231 cells. Electrostatically functionalized MNPs showed effective uptake and DOX linked to MNPs was more efficiently retained inside the cells than free DOX, leading to cell inactivation by mitotic catastrophe, senescence and apoptosis. Both effects, uptake and cytotoxicity, were demonstrated by different assays and videomicroscopy techniques. Likewise, covalently functionalized MNPs using three different linkers—disulfide (DOX-S-S-Pyr, called MF66-S-S-DOX), imine (DOX-I-Mal, called MF66-I-DOX) or both (DOX-I-S-S-Pyr, called MF66-S-S-I-DOX)—were also analysed. The highest cell death was detected using a linker sensitive to both pH and reducing environment (DOX-I-S-S-Pyr). The greatest success of this study was to detect also their activity against breast cancer stem-like cells (CSC) from MDA-MB-231 and primary breast cancer cells derived from a patient with a similar genetic profile (triple-negative breast cancer). In summary, these nanoformulations are promising tools as therapeutic agent vehicles, due to their ability to produce efficient internalization, drug delivery, and cancer cell inactivation, even in cancer stem-like cells (CSCs) from patients.

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Section: Discussionsupporting

confidence: 61%

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Lázaro-Carrillo

Calero

Aires

et al. 2020

Cancers

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“…Over the last two years, the main anticancer drug coupled to IONPs was doxorubicin (DOX) [ 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 ]. This drug can be covalently bound to functionalized IONPs [ 123 ] or establish electrostatic interactions with negatively charged groups present in the magnetic nanocarriers [ 133 ].…”

Section: Drugs Bound To Ionpsmentioning

confidence: 99%

Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity

et al. 2018

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Medical applications and biotechnological advances, including magnetic resonance imaging, cell separation and detection, tissue repair, magnetic hyperthermia and drug delivery, have strongly benefited from employing iron oxide nanoparticles (IONPs) due to their remarkable properties, such as superparamagnetism, size and possibility of receiving a biocompatible coating. Ongoing research efforts focus on reducing drug concentration, toxicity, and other side effects, while increasing efficacy of IONPs-based treatments. This review highlights the methods of synthesis and presents the most recent reports in the literature regarding advances in drug delivery using IONPs-based systems, as well as their antimicrobial activity against different microorganisms. Furthermore, the toxicity of IONPs alone and constituting nanosystems is also addressed.

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“…Various chemotherapeutics have been shown to induce apoptosis and G2/M phase arrest, including monomeric acridine derivatives such as imidazoacridinones and 1-nitroacridines [ 32 , 33 , 34 , 35 ]. We have also shown that Doxorubicin covalently bound to magnetic nanoparticles (Dox-Nps) induces G2/M phase arrest in the human colon HT29 cells, followed by apoptosis and necrosis [ 36 ]. In this paper, we demonstrated that unsymmetrical bisacridines and their hybrids with quantum dots induce apoptosis in human lung H460 and colon HCT116 cells, as revealed by Annexin V/PI staining and changes in mitochondrial membrane potential (ΔΨ m ) ( Figure 6 , Figure 7 , Figures S4 and S5 ).…”

Section: Discussionmentioning

confidence: 99%

Quantum Dots as a Good Carriers of Unsymmetrical Bisacridines for Modulating Cellular Uptake and the Biological Response in Lung and Colon Cancer Cells

et al. 2021

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Nanotechnology-based drug delivery provides a promising area for improving the efficacy of cancer treatments. Therefore, we investigate the potential of using quantum dots (QDs) as drug carriers for antitumor unsymmetrical bisacridine derivatives (UAs) to cancer cells. We examine the influence of QD–UA hybrids on the cellular uptake, internalization (Confocal Laser Scanning Microscope), and the biological response (flow cytometry and light microscopy) in lung H460 and colon HCT116 cancer cells. We show the time-dependent cellular uptake of QD–UA hybrids, which were more efficiently retained inside the cells compared to UAs alone, especially in H460 cells, which could be due to multiple endocytosis pathways. In contrast, in HCT116 cells, the hybrids were taken up only by one endocytosis mechanism. Both UAs and their hybrids induced apoptosis in H460 and HCT116 cells (to a greater extent in H460). Cells which did not die underwent senescence more efficiently following QDs–UAs treatment, compared to UAs alone. Cellular senescence was not observed in HCT116 cells following treatment with both UAs and their hybrids. Importantly, QDgreen/red themselves did not provoke toxic responses in cancer or normal cells. In conclusion, QDs are good candidates for targeted UA delivery carriers to cancer cells while protecting normal cells from toxic drug activities.

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Improved cytotoxicity and preserved level of cell death induced in colon cancer cells by doxorubicin after its conjugation with iron-oxide magnetic nanoparticles

Cited by 37 publications

References 33 publications

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity

Quantum Dots as a Good Carriers of Unsymmetrical Bisacridines for Modulating Cellular Uptake and the Biological Response in Lung and Colon Cancer Cells

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