Improved Detection of Lassa Virus by Reverse Transcription-PCR Targeting the 5′ Region of S RNA

Ölschläger, Stephan; Lelke, Michaela; Emmerich, Petra; Panning, Marcus; Drosten, Christian; Haß, Meike; Asogun, Danny; Ehichioya, Deborah; Omilabu, S.A.; Günther, Stephan

doi:10.1128/jcm.02351-09

Cited by 90 publications

(104 citation statements)

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“…The two remaining strains were LP, the prototypic strain of lineage I from the town of Lassa, and strain Nig05-A08, originating from a patient who had been treated in Irrua. Unfortunately, the virus load was low, so only the sequence of the fragment of the diagnostic GP PCR could be obtained (12). Nig05-A08 clusters with strain LP, although the genetic distance between the two sequences (25% at nucleotide level) is at the upper limit of intralineage diversity (maximum intralineage differences: 21%…”

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Current Molecular Epidemiology of Lassa Virus in Nigeria

et al. 2011

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“…The PTAP late domain motif in the Z protein was changed to PSAP in four of the strains (see Table S2 in the supplemental material). If virus could not be isolated in cell culture, Lassa virus genome fragments were amplified from serum samples of patients by using diagnostic reverse transcription (RT)-PCRs targeting the GP or L gene (12,15) and sequenced.…”

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Current Molecular Epidemiology of Lassa Virus in Nigeria

et al. 2011

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“…The Lassa virus-specific RT-PCR used to confirm the diagnosis is the gold standard for Lassa fever confirmation. Also, the RT-PCR assay currently in use in Nigeria has 100% sensitivity in detecting the local strains of the Lassa virus 11 . She had an admission AST of 101 I.U per liter, a previous study has shown that hospitalized Lassa fever cases with admission AST ≥ 150 IU per liter have increased mortality 6 .…”

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Lassa fever – full recovery without ribavarin treatment: a case report

et al. 2015

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Background: Lassa fever is a rodent-borne zoonosis that clinically manifests as an acute hemorrhagic fever. It is treated using ribavarin. Surviving Lassa fever without receiving the antiviral drug ribavarin is rare. Only few cases have been documented to date. Case Presentation: We report a case of a 59-year old female with fever who was initially thought to have acute pyelonephritis and sepsis syndrome with background malaria. Further changes in her clinical state and laboratory tests led to a suspicion of Lassa fever. However at the time her laboratory confirmatory test for Lassa fever returned, her clinical state had improved and she made full recovery without receiving ribavarin. Her close contacts showed no evidence of Lassa virus infection. Conclusion: This report adds to the literature on the natural history of Lassa fever; and that individuals may survive Lassa fever with conservative management of symptoms of the disease and its complications.

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“…1A). It was used in conjunction with an existing RT-PCR assay targeting the glycoprotein precursor (GPC) gene of Lassa virus (5). To facilitate detection of the PCR products on the array, one primer was synthesized with a 5= biotin and used in excess in the PCR (asymmetric PCR), thus generating biotinylated single-stranded amplicons.…”

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“…Most of the clinical strains had previously been sequenced in the PCR target region (3,5). In addition, in vitro transcripts of 11 reference strains were tested.…”

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confidence: 99%