Improved diagnostic accuracy of malignant neck lumps by a simple BMVC staining assay

Liao, Li‐Jen; Kang, Chi-Chih; Jan, I-Shiow; Chen, Huei-Chin; Wang, Chiung-Lin; Lou, Pei‐Jen; Chang, Ta‐Chau

doi:10.1039/b814417f

Cited by 10 publications

(8 citation statements)

References 11 publications

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“…In addition to the understanding that BMVC can be used as a marker for cancer detection [20], [21], our current study extends the scope of BMVC application from cancer cells to cells whose transformation is ongoing. It demonstrates the potential of BMVC probe of identifying pre-cancerous lesions for early cancer detection.…”

Section: Discussionmentioning

confidence: 73%

“…A BMVC probe can be used to differentiate cancer cells from normal cells [18]. Thus, using a simple handheld device, an acceptable diagnostic accuracy of cancer cells can be instantly achieved, even for a non-specialist [20], [21]. The major advantages of BMVC probes are mainly based on two distinct properties of this fluorescence probe: a significant increase of the fluorescence yield upon interaction with DNA, and the large time lag of adhesion of BMVC to the nucleus between cancer cells and normal cells [21].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

et al. 2014

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Cancer can be easily treated when found early. A probe capable of detecting cell transformation may increase the success rate of early diagnosis of cancer. In this report we have tested the ability of a fluorescent, quadruplex DNA binding probe, 3,6-bis(1-methyl-4- vinylpyridinium) carbazole diiodide (BMVC), to detect cell transformation in vitro. BMVC was applied to living cells in several different models of cell transformation, and the fluorescence signals of BMVC were measured. The degrees of cell transformation in these models were characterized by alterations in cellular morphological phenotype and subcellular organization. When BMVC probes were applied, the number of BMVC-positive cells increased in accordance with the degree of transformation. BMVC was capable of significantly detecting formation of foci, increased cellular motility, cell proliferation, cell apoptosis, anchorage-independent growth, and increased invasiveness of transformed cells. These results demonstrate the ability of BMVC probes to detect cell transformation and indicate that BMVC is of promise for use as a probe in early cancer detection.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

et al. 2014

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“…Dr. Chang demonstrated a novel method based on emulsion-induced filtration with the aid of BMVC-12C-Br to identify and separate G4 structures from duplex DNA and parallel G4 structures from antiparallel or hybrid forms. He also discussed how fluorescence imaging microscopy can be used for cancer diagnosis due to differential accumulation of BMVC and its analogs in cancer (preferentially in the nucleus and mitochondria) compared with normal (in the lysosomes) cells [62]. In addition, Dr. Chang demonstrated that combining PEG unit or n- ethylene glycol with known G4 stabilizers leads to new molecules capable of inducing controlled structural switches between different G4 topologies and increasing the melting temperature of human telomeres by more than 45 °C.…”

Section: Discussionmentioning

confidence: 99%

G-ruption: The third international meeting on G-quadruplex and G-assembly

2012

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A three and a half day conference focusing on nucleic acid structures called G-quadruplexes (G4s) and other guanine-based assemblies was held in Sorrento, Italy (June 28–July 1, 2011) and featured 35 invited talks and over 89 posters. The G-quadruplex field continues to expand at an explosive rate with the emergence of new connections to biology, chemistry, physics, and nanotechnology. Following the trend established by the previous two international G4 meetings, the conference touched upon all these areas and facilitated productive exchanges of ideas between researchers from all over the world.

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“…Recently, it has become of interest in developing fluorescence probes, which can selectively illuminate malignant cells . Among them, a small organic compound, 3,6‐Bis(1‐methyl‐4‐vinylpyridinium)carbazole diiodide (BMVC), shows a strong fluorescence contrast between cancer cells and normal cells due to two distinct properties: a significant increase of the fluorescence quantum yield upon interaction with DNA and a dramatic difference in the intracellular localization between cancer cells and normal cells .…”

Section: Introductionmentioning

confidence: 99%

BMVC test, an improved fluorescence assay for detection of malignant pleural effusions

et al. 2014

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The diagnosis of malignant pleural effusions is an important issue in the management of malignancy patients. Generally, cytologic examination is a routine diagnostic technique. However, morphological interpretation of cytology is sometimes inconclusive. Here an ancillary method named BMVC test is developed for rapid detection of malignant pleural effusion to improve the diagnostic accuracy at low cost. A simple assay kit is designed to collect living cells from clinical pleural effusion and a fluorescence probe, 3,6-Bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC), is used to illuminate malignant cells. The fluorescence intensity is quantitatively analyzed by ImageJ program. This method yields digital numbers for the test results without any grey zone or ambiguities in the current cytology tests due to intra-observer and inter-observer variability. Comparing with results from double-blind cytologic examination, this simple test gives a good discrimination between malignant and benign specimens with sensitivity of 89.4% (42/47) and specificity of 93.3% (56/60) for diagnosis of malignant pleural effusion. BMVC test provides accurate results in a short time period, and the digital output could assist cytologic examination to become more objective and clear-cut. This is a convenient ancillary tool for detection of malignant pleural effusions.

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Improved diagnostic accuracy of malignant neck lumps by a simple BMVC staining assay

Cited by 10 publications

References 11 publications

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

Detection of Cell Carcinogenic Transformation by a Quadruplex DNA Binding Fluorescent Probe

G-ruption: The third international meeting on G-quadruplex and G-assembly

BMVC test, an improved fluorescence assay for detection of malignant pleural effusions

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