Improved Direct Electrochemistry for Proteins Adsorbed on a UV/Ozone-Treated Carbon Nanofiber Electrode

Xue, Qi‐Kun; Kato, Dai; Kamata, Toshihide; Guo, Qiaohui; You, Tianyan; Niwa, Osamu

doi:10.2116/analsci.29.611

Cited by 19 publications

(12 citation statements)

References 45 publications

(52 reference statements)

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“…The differences in terms of amino-acid sequence between bacterial BODs and fungal BODs also resulted in different behaviors. 434 Due to the difficulty to rationalize how DET with Mv-BOD could be obtained on carbon surfaces, which generally bear lots of functionalities and have a porous structure, 460 studies were performed on well-ordered and atomically flat single gold crystal 151 and then extended to carbon or more complex architectures: KB, 461 GO, ECR-sputtered CNFs 431 and CNTs. 152,216,338,437,462,463 Here also DET was proved because the catalysis started at a potential that could be attributed to the T1 whatever the chemical functions at the electrode surface, 151 even when the surface was modified with redox active groups.…”

Section: Bilirubin Oxidasesmentioning

confidence: 99%

O₂Reduction in Enzymatic Biofuel Cells

2017

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Catalytic four-electron reduction of O to water is one of the most extensively studied electrochemical reactions due to O exceptional availability and high O/HO redox potential, which may in particular allow highly energetic reactions in fuel cells. To circumvent the use of expensive and inefficient Pt catalysts, multicopper oxidases (MCOs) have been envisioned because they provide efficient O reduction with almost no overpotential. MCOs have been used to elaborate enzymatic biofuel cells (EBFCs), a subclass of fuel cells in which enzymes replace the conventional catalysts. A glucose/O EBFC, with a glucose oxidizing anode and a O reducing MCO cathode, could become the in vivo source of electricity that would power sometimes in the future integrated medical devices. This review covers the challenges and advances in the electrochemistry of MCOs and their use in EBFCs with a particular emphasis on the last 6 years. First basic features of MCOs and EBFCs are presented. Clues provided by electrochemistry to understand these enzymes and how they behave once connected at electrodes are described. Progresses realized in the development of efficient biocathodes for O reduction relying both on direct and mediated electron transfer mechanism are then discussed. Some implementations in EBFCs are finally presented.

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Section: Bilirubin Oxidasesmentioning

confidence: 99%

O₂Reduction in Enzymatic Biofuel Cells

2017

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“…Carbon nanomaterials with excellent properties and unique structure have been widely utilized in the study of direct electrochemistry of enzymes 14 15 16 17 . For instance, Niwa’s group have prepared a novel carbon film via UV/ozone treatment, which realized the efficient DET of cytochrome c 18 .…”

mentioning

confidence: 99%

Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film

Zhang

Liu

et al. 2015

Sci Rep

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We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepared by controlled thermal treatment of electrospun polypyrrole nanospheres doped polyacrylonitrile nanofibers (PPyNSs@PAN NFs). Without any pretreatment, the as-prepared material can directly serve as a platform for GOx immobilization. The cyclic voltammetry of immobilized GOx showed a pair of well-defined redox peaks in O2-free solution, indicating the DET of GOx. With the addition of glucose, the anodic peak current increased, while the cathodic peak current decreased, which demonstrated the DET-based bioelectrocatalysis. The detection of glucose based on the DET of GOx was achieved, which displayed high sensitivity, stability and selectivity, with a low detection limit of 2 μM and wide linear range of 12–1000 μM. These results demonstrate that the as-obtained NCNSs@CNFs can serve as an ideal platform for the construction of the third-generation glucose biosensor.

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“…It is, therefore, likely that our oxygen-terminated B-UNCD and B-MCD electrodes contained C-OH and C-O-C functional groups with lower levels of COOH groups due to the absence of a significant effect of pH on the cyclic voltammetrically determined reduction peak current, k o app , E 1/2 and ΔE p . Xue et al [76] reported how the UV/ozone treatment of carbon nanofiber electrodes improved the direct electron transfer of cytochrome c as compared to the untreated surface. The pretreatment increased the hydrophilicity of the surface, as measured by water contact angle, and the surface carbon-oxygen functional group content.…”

Section: Discussionmentioning

confidence: 99%

Effects of Film Morphology and Surface Chemistry on the Direct Electrochemistry of Cytochrome c at Boron-Doped Diamond Electrodes

Dai

Proshlyakov

Swain

2016

Electrochimica Acta

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The effects of film morphology and surface termination on the direct electron transfer of horse heart cytochrome c on boron-doped ultrananocrystalline (B-UNCD) and microcrystalline (B-MCD) diamond thin-film electrodes were investigated. Quasi-reversible, diffusion-controlled cyclic voltammetric responses were observed on oxygen-terminated (atomic O/C ~0.015), but not hydrogen-terminated (atomic O/C ~0.02) diamond thin films. The effect of the surface termination was the same for both the nanostructured B-UNCD film with sp2-bonded carbon atoms in the grain boundaries and the well faceted B-MCD film with micron-sized grains and largely devoid of sp2 carbon. Stable cyclic voltammetric i-E curves were recorded with cycling for both oxygen-terminated films indicating the absence of protein denaturation and electrode fouling. The peak currents increased linearly with the square root of the scan rate and the protein concentration; both indicative of a reaction rate limited by semi-infinite linear diffusion of the protein. Similar heterogeneous electron-transfer rate constants were observed for oxygen-terminated B-UNCD (3.48 (± 1.25) × 10−3 cm/s) and B-MCD films (2.38 (± 0.72) × 10−3 cm/s). The results clearly reveal that the oxygen-terminated surface is more active for electron-transfer with this soluble redox protein than is the hydrogen-terminated surface. The film morphology does not influence the diffusion-controlled response of the redox protein.

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Improved Direct Electrochemistry for Proteins Adsorbed on a UV/Ozone-Treated Carbon Nanofiber Electrode

Cited by 19 publications

References 45 publications

O₂Reduction in Enzymatic Biofuel Cells

O₂Reduction in Enzymatic Biofuel Cells

Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film

Effects of Film Morphology and Surface Chemistry on the Direct Electrochemistry of Cytochrome c at Boron-Doped Diamond Electrodes

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Improved Direct Electrochemistry for Proteins Adsorbed on a UV/Ozone-Treated Carbon Nanofiber Electrode

Cited by 19 publications

References 45 publications

O2Reduction in Enzymatic Biofuel Cells

O2Reduction in Enzymatic Biofuel Cells

Direct Electrochemistry of Glucose Oxidase on Novel Free-Standing Nitrogen-Doped Carbon Nanospheres@Carbon Nanofibers Composite Film

Effects of Film Morphology and Surface Chemistry on the Direct Electrochemistry of Cytochrome c at Boron-Doped Diamond Electrodes

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O₂Reduction in Enzymatic Biofuel Cells

O₂Reduction in Enzymatic Biofuel Cells