Improved Draft Genome Sequence of
            <i>Microbacterium</i>
            sp. Strain LKL04, a Bacterial Endophyte Associated with Switchgrass Plants

Sahib, Mohammad Radhi; Yang, Piao; Bokros, Norbert; Shapiro, Nicole; Woyke, Tanja; Kyrpides, Nikos C.; Xia, Ye; DeBolt, Seth

doi:10.1128/mra.00927-19

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…These microbes can promote plant growth and enhance plant defense by providing essential nutrients, protecting them from harmful pathogens, and increasing their resistance to abiotic stresses [9,176]. More and more studies have proved the significance and potential of beneficial microbes in promoting plant fitness [9,174,[177][178][179]. Therefore, we propose a modified model called the plant fitness tetrahedron by integrating the beneficial microbes (Figure 5B).…”

Section: Future Directions On Preventive Management Of P Syringaementioning

confidence: 99%

“…Therefore, we propose a modified model called the plant fitness tetrahedron by integrating the beneficial microbes (Figure 5B). The presence of beneficial microbes is essential for the health and fitness of plants, and their importance has been increasingly recognized in agriculture and plant science [9,174,[177][178][179]. There are several well-known ways in which beneficial microbes can enhance plant fitness.…”

Section: Future Directions On Preventive Management Of P Syringaementioning

confidence: 99%

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Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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Plant diseases caused by the pathogen Pseudomonas syringae are serious problems for various plant species worldwide. Accurate detection and diagnosis of P. syringae infections are critical for the effective management of these plant diseases. In this review, we summarize the current methods for the detection and diagnosis of P. syringae, including traditional techniques such as culture isolation and microscopy, and relatively newer techniques such as PCR and ELISA. It should be noted that each method has its advantages and disadvantages, and the choice of each method depends on the specific requirements, resources of each laboratory, and field settings. We also discuss the future trends in this field, such as the need for more sensitive and specific methods to detect the pathogens at low concentrations and the methods that can be used to diagnose P. syringae infections that are co-existing with other pathogens. Modern technologies such as genomics and proteomics could lead to the development of new methods of highly accurate detection and diagnosis based on the analysis of genetic and protein markers of the pathogens. Furthermore, using machine learning algorithms to analyze large data sets could yield new insights into the biology of P. syringae and novel diagnostic strategies. This review could enhance our understanding of P. syringae and help foster the development of more effective management techniques of the diseases caused by related pathogens.

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Section: Future Directions On Preventive Management Of P Syringaementioning

confidence: 99%

Section: Future Directions On Preventive Management Of P Syringaementioning

confidence: 99%

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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“…In addition to their direct impact on inhibiting pathogens, beneficial bacteria also indirectly manage plant pathogens by modulating host plant immunity through mechanisms like ISR and SAR [ 46 , 47 , 57 , 58 ]. ISR and SAR are two different types of systemic plant defense mechanisms.…”

Section: Resultsmentioning

confidence: 99%

“…Traditional microbial and genomic methods have proven valuable for screening potential beneficial bacteria from the environments of host plants [ 15 , 57 , 58 ]. However, before these isolated beneficial bacteria can be effectively applied in agriculture, medicine, or industry, in-depth functional characterization is essential.…”

Section: Introductionmentioning

confidence: 99%

Plant Growth Promotion and Plant Disease Suppression Induced by Bacillus amyloliquefaciens Strain GD4a

Yang,

Yuan,

Liu

et al. 2024

Plants

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Botrytis cinerea, the causative agent of gray mold disease (GMD), invades plants to obtain nutrients and disseminates through airborne conidia in nature. Bacillus amyloliquefaciens strain GD4a, a beneficial bacterium isolated from switchgrass, shows great potential in managing GMD in plants. However, the precise mechanism by which GD4a confers benefits to plants remains elusive. In this study, an A. thaliana-B. cinerea-B. amyloliquefaciens multiple-scale interaction model was used to explore how beneficial bacteria play essential roles in plant growth promotion, plant pathogen suppression, and plant immunity boosting. Arabidopsis Col-0 wild-type plants served as the testing ground to assess GD4a’s efficacy. Additionally, bacterial enzyme activity and targeted metabolite tests were conducted to validate GD4a’s potential for enhancing plant growth and suppressing plant pathogens and diseases. GD4a was subjected to co-incubation with various bacterial, fungal, and oomycete pathogens to evaluate its antagonistic effectiveness in vitro. In vivo pathogen inoculation assays were also carried out to investigate GD4a’s role in regulating host plant immunity. Bacterial extracellular exudate (BEE) was extracted, purified, and subjected to untargeted metabolomics analysis. Benzocaine (BEN) from the untargeted metabolomics analysis was selected for further study of its function and related mechanisms in enhancing plant immunity through plant mutant analysis and qRT-PCR analysis. Finally, a comprehensive model was formulated to summarize the potential benefits of applying GD4a in agricultural systems. Our study demonstrates the efficacy of GD4a, isolated from switchgrass, in enhancing plant growth, suppressing plant pathogens and diseases, and bolstering host plant immunity. Importantly, GD4a produces a functional bacterial extracellular exudate (BEE) that significantly disrupts the pathogenicity of B. cinerea by inhibiting fungal conidium germination and hypha formation. Additionally, our study identifies benzocaine (BEN) as a novel small molecule that triggers basal defense, ISR, and SAR responses in Arabidopsis plants. Bacillus amyloliquefaciens strain GD4a can effectively promote plant growth, suppress plant disease, and boost plant immunity through functional BEE production and diverse gene expression.

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“…Beyond these foundational merits, the envisioned model substantiates its potency through the augmentation of the plant's immune competence, heralding a triad of pivotal defense mechanisms: induced systemic resistance (ISR), systemic acquired resistance (SAR), and herbivore-induced resistance (HIR). In summation, this proposed model stands as an eloquent testament to the symphonic choreography between beneficial microbes (such as OSUB18, LKL04 [54], RRD69 [55], and GD4a [56]) and the host plants (such as Arabidopsis [22], tomato [8,9], and strawberry [57] plants), ushering in a transformative epoch in agriculture characterized by heightened yields, fortified resilience against adversities, and the cultivation of sustainable agricultural practices. A proposed model of beneficial microbe-mediated plant protection.…”

Section: Discussionmentioning

confidence: 99%

Plant Growth Promotion and Stress Tolerance Enhancement through Inoculation with Bacillus proteolyticus OSUB18

Yang,

Liu,

Yuan

et al. 2023

Biology

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The isolation of B. proteolyticus OSUB18 from switchgrass unveiled its significant potential in both the enhancement of plant growth and the suppression of plant diseases in our previous study. The elucidation of the related mechanisms governing this intricate plant–microbe interaction involved the utilization of the model plant Arabidopsis thaliana. In our comprehensive study on Arabidopsis, OSUB18 treatment was found to significantly alter root architecture and enhance plant growth under various abiotic stresses. An RNA-seq analysis revealed that OSUB18 modified gene expression, notably upregulating the genes involved in glucosinolate biosynthesis and plant defense, while downregulating those related to flavonoid biosynthesis and wound response. Importantly, OSUB18 also induces systemic resistance in Arabidopsis against a spectrum of bacterial and fungal pathogens and exhibits antagonistic effects on phytopathogenic bacteria, fungi, and oomycetes, highlighting its potential as a beneficial agent in plant stress management and pathogen resistance. Overall, our findings substantiate that OSUB18 exerts a stimulatory influence on plant growth and health, potentially attributed to the remodeling of root architecture, defense signaling, and the comprehensive mitigation of various biotic and abiotic stresses.

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Improved Draft Genome Sequence of Microbacterium sp. Strain LKL04, a Bacterial Endophyte Associated with Switchgrass Plants

Cited by 10 publications

References 26 publications

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Plant Growth Promotion and Plant Disease Suppression Induced by Bacillus amyloliquefaciens Strain GD4a

Plant Growth Promotion and Stress Tolerance Enhancement through Inoculation with Bacillus proteolyticus OSUB18

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