Improved ELISA for linoleate-derived diols in human plasma utilizing a polyHRP-based secondary tracer

Singh, Nalin; Li, Dongyang; McReynolds, Cindy B.; Morisseau, Christophe; Hammock, Bruce D.

doi:10.1039/d2ay00272h

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…Thus, the high sensitivity of the assay allows dilution to be used to eliminate matrix effects of human urine rather than the added expense of partitions or chromatographic clean up. Here, 10-fold dilution of human urine was selected as in most publications [ 34 ]. A similar analysis was performed with the serum matrix for EC5026 and TPPU assays.…”

Section: Resultsmentioning

confidence: 99%

Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA

Yang

Meng

et al. 2023

Journal of Pharmaceutical Analysis

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Section: Resultsmentioning

confidence: 99%

Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA

Yang

Meng

et al. 2023

Journal of Pharmaceutical Analysis

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“…Colon tissue samples were extracted by mixing with methanol and then washed with hexanes as previously stated [52]. Briefly, the extractions were diluted with PBS for the ELISA analysis.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa) Analysismentioning

confidence: 99%

Soluble Epoxide Hydrolase Contributes to Cell Senescence and ER Stress in Aging Mice Colon

Wang

Wagner

Wang

et al. 2023

IJMS

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Aging, which is characterized by enhanced cell senescence and functional decline of tissues, is a major risk factor for many chronic diseases. Accumulating evidence shows that age-related dysfunction in the colon leads to disorders in multiple organs and systemic inflammation. However, the detailed pathological mechanisms and endogenous regulators underlying colon aging are still largely unknown. Here, we report that the expression and activity of the soluble epoxide hydrolase (sEH) enzyme are increased in the colon of aged mice. Importantly, genetic knockout of sEH attenuated the age-related upregulation of senescent markers p21, p16, Tp53, and β-galactosidase in the colon. Moreover, sEH deficiency alleviated aging-associated endoplasmic reticulum (ER) stress in the colon by reducing both the upstream regulators Perk and Ire1 as well as the downstream pro-apoptotic effectors Chop and Gadd34. Furthermore, treatment with sEH-derived linoleic acid metabolites, dihydroxy-octadecenoic acids (DiHOMEs), decreased cell viability and increased ER stress in human colon CCD-18Co cells in vitro. Together, these results support that the sEH is a key regulator of the aging colon, which highlights its potential application as a therapeutic target for reducing or treating age-related diseases in the colon.

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Nanobody based immunoassay for alpha fetal protein detection using streptavidin-conjugated polymerized horseradish peroxidase for signal amplification

Sun,

Liu,

Bao

et al. 2023

ANAL. SCI.

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Improved ELISA for linoleate-derived diols in human plasma utilizing a polyHRP-based secondary tracer

Abstract: Dihydroxyoctadecenoic acids (DiHOMEs) are cytochrome P450 pathway-derived metabolites of linoleic acid, a highly abundant dietary fatty acid. They serve thermogenic functions at low concentrations but, at high concentrations, are involved...

Cited by 4 publications

References 39 publications

Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA

Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA

Soluble Epoxide Hydrolase Contributes to Cell Senescence and ER Stress in Aging Mice Colon

Nanobody based immunoassay for alpha fetal protein detection using streptavidin-conjugated polymerized horseradish peroxidase for signal amplification

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