Improved endothelialization and reduced thrombosis by coating a synthetic vascular graft with fibronectin and stem cell homing factor SDF-1α

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

“…Growth factors such as VEGF, 42 stromal cell-derived factor-1 (SDF-1), 43 and granulocyte colony-stimulating factor (G-CSF) 44 have been found to increase mobilization of EPCs from the bone marrow and used in vascular graft applications. Nerve growth factor (NGF) has also been found to promote EPC migration in vitro and mobilization and homing in vivo to a collagen-modified decellularized blood vessel matrix.…”

“…43 Grafts were coated with fibronectin and, subsequently, homing factor SDF-1a to promote homing and adhesion of hematopoietic stem cells and EPCs. Significantly, less thrombotic material accumulated on both grafts after coating, and EC coverage of the grafts was almost doubled compared to the uncoated controls.…”

Extract of fetal membrane would inhibit thrombosis and hemolysis

“…autologous venous endothelial and endothelial progenitor or mesothelial cells. Harvesting of these endothelial has difficulties due to high costs, increasing the rate of infection and lack of appropriate number of cells [36]. Currently, stem cells have been targeted to be a potent and renewable source of endothelial cells based on their capabilities to differentiate into any cells of three germ layers [37].…”

Vascular Grafting Strategies in Coronary Intervention

“…The authors observed an increase in endothelialization from 27 ± 4 to 48 ± 4% with the SDF-1α coating compared to vascular grafts alone in an ovine model. Concurrently, they also observed a decrease in intimal hyperplasia (De Visscher et al, 2012).…”