Improved glucose label-free biosensor with layer-by-layer architecture and conducting polymer poly(3,4-ethylenedioxythiophene)

David, Melinda; Bârsan, Mădălina M.; Brett, Christopher M. A.; Florescu, Monica

doi:10.1016/j.snb.2017.09.149

Cited by 56 publications

(27 citation statements)

References 29 publications

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“…In the form of Nyquist plots, EIS was employed to clarify the interface properties of PEDOT, PANI and PEDOT/PANI (Figure (B)). The diameter of the semicircle of the electron transfer limited process, is equivalent to the charge transfer resistance (Rct) . Compared to GCE and GCE/PANI, the Rcts of GCE/PEDOT and GCE/PEDOT/PANI have been greatly reduced.…”

Section: Resultsmentioning

confidence: 99%

Electrodeposition of Three‐Dimensional Network Nanostructure PEDOT/PANI for Simultaneous Voltammetric Detection of Ascorbic Acid, Dopamine and Uric Acid

et al. 2020

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In this study, poly(3,4-ethylenedioxythiophene) (PEDOT) and polyaniline (PANI) were electrodeposited via cyclic voltammetry (CV) on glass carbon electrode (GCE) layer by layer. The prepared electrode (GCE/PEDOT/PANI) was used for the simultaneous sensitive determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA) at different oxidation potentials. The peak distances of AA and DA, DA and UA, AA and UA were 216 mV, 104 mV, 320 mV, respectively. For individual detection of AA, DA and UA, the linear ranges were 1 × 10 À 4 -1 × 10 À 2 M, 3 × 10 À 5 -1 × 10 À 3 M and 7 × 10 À 7 -1 × 10 À 4 M with detection limits of 2.42 × 10 À 5 M, 4.58 × 10 À 6 M and 2.38 × 10 À 7 M (S/N = 3), respectively. For simultaneous detection of AA, DA, and UA, the linear response ranges were 1 × 10 À 4 -1 × 10 À 3 M, 3 × 10 À 4 -1 × 10 À 2 M, and 3 × 10 À 6 -1 × 10 À 4 M with detection limits of 6.91 × 10 À 5 M, 3.66 × 10 À 5 M and 4.13 × 10 À 7 M (S/ N = 3), respectively. The GCE/PEDOT/PANI displayed good selectivity, reproducibility and storage stability, and was further applied to the detection of AA, DA, and UA in 5% normal human serum samples.

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Section: Resultsmentioning

confidence: 99%

Electrodeposition of Three‐Dimensional Network Nanostructure PEDOT/PANI for Simultaneous Voltammetric Detection of Ascorbic Acid, Dopamine and Uric Acid

et al. 2020

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“…[52] In the case of layer-by-layer assembly of nanographene/GOx/Chitosan alternated with polystyrene sulfonate (PSS) on gold modified with PEDOT, SEM and AFM were associated to determine the surface characteristics of the layers and their thickness. [53] AFM imaging after immuno-labelling also attested the adsorption of horseradish peroxidase (HRP) on a gold (111) surface decorated with a phospholipid bilayer, consistently with negatively charged phospholipids and positively charged HRP at the experimental pH (6.5). A primary antibody allowed enzyme recognition, while the secondary antibody bore nanoparticles clearly visible as white dots on the AFM image ( Figure 2).…”

Section: Afmmentioning

confidence: 89%

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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Redox enzymes, which catalyze electron transfer reactions in living organisms, can be used as selective and sensitive bioreceptors in biosensors, or as efficient catalysts in biofuel cells. In these bioelectrochemical devices, the enzymes are immobilized at a conductive surface, the electrode, with which they must be able to exchange electrons. Different physicochemical methods have been coupled to electrochemistry to characterize the enzyme‐modified electrochemical interface. In this Review, we summarize most efforts performed to investigate the enzymatic electrodes at the micro‐ and even nanoscale, thanks to microscopy techniques. Contrary to electrochemistry, which gives only a global information about all processes occurring at the electrode surface, microscopy offers a spatial resolution. Several techniques have been implemented; mostly scanning probe microscopies like atomic force microscopy, scanning tunneling microscopy, and scanning electrochemical microscopy, but also scanning electron microscopy and fluorescence microscopy. These studies demonstrate that various information can be obtained thanks to microscopy at different scales. Electrode imaging has been performed to confirm the presence of enzymes, to quantify and localize the biomolecules, but also to evaluate the morphology of immobilized enzymes, their possible conformation changes upon turnover, and their orientation at the electrode surface. Local redox activity has also been imaged and kinetics has been resolved.

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“…Similarly, highly sensitive CP-based glucose biosensors, which are relevant for patients suffering from Diabetes's disease, have also been developed using glucose oxidase (GOx) enzyme. The use of GOx-loaded PtNPs-PEDOT microspheres has shown the best reported sensor of PEDOT up to date (Piro et al, 2001;Nien et al, 2006;David et al, 2018;Liu et al, 2018).…”

Section: Use Of Conductive Polymersmentioning

confidence: 99%

Tunable Polymeric Scaffolds for Enzyme Immobilization

Rodriguez‐Abetxuko

Sánchez-deAlcázar

Muñumer

et al. 2020

Front. Bioeng. Biotechnol.

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The number of methodologies for the immobilization of enzymes using polymeric supports is continuously growing due to the developments in the fields of biotechnology, polymer chemistry, and nanotechnology in the last years. Despite being excellent catalysts, enzymes are very sensitive molecules and can undergo denaturation beyond their natural environment. For overcoming this issue, polymer chemistry offers a wealth of opportunities for the successful combination of enzymes with versatile natural or synthetic polymers. The fabrication of functional, stable, and robust biocatalytic hybrid materials (nanoparticles, capsules, hydrogels, or films) has been proven advantageous for several applications such as biomedicine, organic synthesis, biosensing, and bioremediation. In this review, supported with recent examples of enzyme-protein hybrids, we provide an overview of the methods used to combine both macromolecules, as well as the future directions and the main challenges that are currently being tackled in this field.

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Improved glucose label-free biosensor with layer-by-layer architecture and conducting polymer poly(3,4-ethylenedioxythiophene)

Cited by 56 publications

References 29 publications

Electrodeposition of Three‐Dimensional Network Nanostructure PEDOT/PANI for Simultaneous Voltammetric Detection of Ascorbic Acid, Dopamine and Uric Acid

Electrodeposition of Three‐Dimensional Network Nanostructure PEDOT/PANI for Simultaneous Voltammetric Detection of Ascorbic Acid, Dopamine and Uric Acid

Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

Tunable Polymeric Scaffolds for Enzyme Immobilization

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