Improved HPLC Method for the Determination of Moxifloxacin in Application to a Pharmacokinetics Study in Patients with Infectious Diseases

Abstract

Objective. To develop a simple and rapid high-performance liquid chromatography (HPLC) method for measuring moxifloxacin concentration in human plasma. Methods. Following a single step liquid-liquid extraction, analytes along with an internal standard (IS) were separated using an isocratic mobile phase of 0.1% triethylamine (adjusted pH to 4.8 with phosphoric acid)/acetonitrile (80/20, v/v) at flow rate of 1 mL/min on reverse phase Kromasil C18 column (250 mm × 4.6 mm, 5 μm) at room temperature. Results. Total analytical run time for selecting moxifloxacin was 15 min. The assays exhibited good linearity (r 2 = 0.9998) over the studied range of 25 to 5000 ng/mL. The absolute recovery rate of low, medium, and high concentrations were 69.88%, 78.86%, and 78.51%, respectively. The relative recovery rates were 98.50%, 96.61%, and 101.79%, respectively. Coefficient of variation and error at both of the intraday and interday assessments were less than 4.7%. Conclusions. The results indicated that this method is a simple, rapid, precise and accurate assay for the determination of moxifloxacin concentrations in human plasma. This validated method is sensitive and reproducible enough to be used in pharmacokinetic studies.

High Performance Liquid Chromatography Assay for Determination of Moxifloxacin in Human Plasma

“…On the other side, HPLC with UV detector was employed for applications in routine therapeutic drug monitoring or clinical pharmacokinetic studies of moxifloxacin. Most reported HPLC-UV methods involve a sample pretreatment step in which methanol [8], acetonitrile [9,10] or dichloromethane [11] was used as precipitating solvent. Alternatively, in this paper, we report a simple HPLC-UV method for determination of moxifloxacin in plasma using trichloroacetic acid within deproteinization step.…”

“…Chromatographic analysis was performed on HPLC system (Shimadzu, Japan) with a PDA detector and using a MRC-ODS C18 column, 250 × 4.5 mm, 5 μm. The chromatographic conditions were achieved in this study according to the Liqin Zhu's method [10]. The mobile phase was a mixing of 20 mM phosphate buffer (pH 4.0 adjusted by phosphoric acid) and acetonitrile with a ratio 30:70 (v/v), delivered at 0.8 mL/min rate.…”

Kinetic Spectrophotometric Determination of Drugs Based On Oxidation by Alkaline Kmno4

“…Moxifloxacin (MOX) [ Fig.1 b] chemically known as l-cyclopropyl-6-fluoro-l, 4-dihydro-8-methoxy-7-[(4aS, 7aS)-octahydro-6H-pyrrolo-[3, 4-b] pyridin-6-yl]-4-oxo-3-quinolinecarboxylic acid is a fourth generation fluoroquinolone broad spectrum antibiotic agent used in Conjunctivitis [7,8]. Some methods such as HPLC [9,10], Fluorimetry [11,12], spectrophotometry [13,14] and Voltammetry [15] are used for the determination of MOX.…”

Photodegradation of Moxifloxacin in Aqueous and Organic Solvents: A Kinetic Study

“…Chromatographic methods including high-performance liquid chromatography (HPLC) (18,(28)(29)(30)(31)(32)(33)(34)(35), ultra-performance liquid chromatography (UPLC) (19) and densitometric highperformance thin-layer chromatography (HPTLC) (36) have been used for the assay of MF and detection of its chemical and photodegradation products (19,29,33,34,36,37). Some photodegradation products of MF in aqueous solution have been identified by HPLC, NMR, and MS studies (18,19,21,22).…”