Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media

Lim, Kye-Taek; Jang, Goo; Ko, Kwang–Hoon; Lee, Won Wou; Park, Hee Jung; Kim, Jung-Joo; Lee, So Hyun; Hwang, Woo Suk; Lee, Byeong Chun; Kang, Sung Keun

doi:10.1016/j.theriogenology.2006.07.011

Cited by 83 publications

(68 citation statements)

References 38 publications

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“…In this sense, some authors observed no beneficial effects of EGF in the embryo CM on the blastocyst rate either in individual (mouse: O'Neill, 1997, 0.2 to 2000 ng/ ml; bovine: Keefer et al, 1994, 10 ng/ml) or in group culture (bovine: Flood et al, 1993, 10 ng/ml, Shamsuddin, 1994, 10 to 50 ng/ml, Lee and Fukui, 1995, 10 ng/ml). However, other authors observed differences both in single (mouse: Paria and Dey, 1990, 10 ng/ml; bovine: Lim et al, 2007, 10 ng/ml) and group culture (bovine: Lonergan et al, 1996, 100 ng/ml; Mtango et al, 2003, 10 ng/ml;, 5 ng/ml). On the other hand, the effect of TGF-a was also evaluated in the present work, and no beneficial effects, but rather negative, of this GF on embryo development were found, confirming results previously showed by other authors observing no beneficial effects (Brison and Schultz, 1997;Zhou et al, 2008).…”

Section: Discussionmentioning

confidence: 87%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Section: Discussionmentioning

confidence: 87%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…This has led on one side to the use of chemically defined media containing growth factors [33], on the other side to the development of culture systems employing co-culturing with growth factor-releasing cells [47], so that dramatic improvements in the composition of embryo culture media have been made in the last 15 years allowing the efficient production of viable human blastocysts.…”

Section: Discussionmentioning

confidence: 99%

“…Among others, supplementation of culture media with myoIns has produced positive effects on the development of rabbit [51] and bovine [20,33] preimplantation embryos. We hypothesized that similar effects may be exerted by myoIns on human preimplantation embryos, resulting in an increase in embryo viability and possibly representing a step forward in the evolution in culture media used in clinical IVF.…”

Section: Discussionmentioning

confidence: 99%

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Colazingari

Fiorenza

Carlomagno³

et al. 2014

J Assist Reprod Genet

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Objective Myo-inositol (myoIns) has a positive role in mammalian development and human reproduction. Since experiments on farming species suggest a similar role in preimplantation development, we evaluated the hypothesis that the inclusion of myoIns in human embryo culture media would produce an increase in embryo quality in IVF cycles, using the mouse embryo assay. Methods To determine the effect of myoIns on completion of preimplantation development in vitro, one-cell embryos of the inbred C57BL/6N mouse strain were produced by ICSI, cultured in human fertilization media in the presence of myoIns (myoIns+) or in its absence (myoIns-) and evaluated morphologically. Daily progression through cleavage stages, blastocyst production and expansion and blastomere number at 96 hours post fertilization were assessed. Results Compared to myoIns-embryos, myoIns+ embryos displayed a faster cleavage rate and by the end of preimplantation development, the majority of myoIns+ blastocysts was expanded and formed by a higher number of blastomeres. Conclusion The presence of myoIns resulted in both an increase in proliferation activity and developmental rate of in vitro cultured early mouse embryos, representing a substantial improvement of culture conditions. These data may identify myoIns as an important supplement for human embryo preimplantation culture.

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“…Despite great advances in the design of defined and semidefined embryo culture media [4][5][6], researchers still seek new media additives and somatic cell-based co-culture systems in an attempt to obtain higher yields of better quality embryos [7][8][9][10]. Presumably, somatic cells in a co-culture system may remove toxic components and reactive oxygen species, as well as Capsule Co-culture of in vitro produced bovine embryos with adult adipose tissue-derived bovine stem cells improved blastocyst development and quality.…”

Section: Introductionmentioning

confidence: 99%

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Miranda

Nascimento

Costa

et al. 2016

J Assist Reprod Genet

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Purpose Despite advances in the composition of defined embryo culture media, co-culture with somatic cells is still used for bovine in vitro embryo production (IVEP) in many laboratories worldwide. Granulosa cells are most often used for this purpose, although recent work suggests that co-culture with stem cells of adult or embryonic origin or their derived biomaterials may improve mouse, cattle, and pig embryo development. Materials and methods In experiment 1, in vitro produced bovine embryos were co-cultured in the presence of two concentrations of bovine adipose tissue-derived mesenchymal cells (b-ATMSCs; 10 3 and 10 4 cells/mL), in b-ATMSC preconditioned medium (SOF-Cond), or SOF alone (control). In experiment 2, co-culture with 10 4 b-ATMSCs/mL was compared to the traditional granulosa cell co-culture system (Gran). Results In experiment 1, co-culture with 10 4 b-ATMSCs/mL improved blastocyst rates in comparison to conditioned and control media (p < 0.05). Despite that it did not show difference with 10 3 b-ATMSCs/mL (p = 0.051), group 10 4 bATMSCs/mL yielded higher results of blastocyst production.In experiment 2, when compared to group Gran, co-culture with 10 4 b-ATMSCs/mL improved not only blastocyst rates but also quality as assessed by increased total cell numbers and mRNA expression levels for POU5F1 and G6PDH (p < 0.05). Conclusions Co-culture of bovine embryos with b-ATMSCs was more beneficial than the traditional co-culture system with granulosa cells. We speculate that the microenvironmental modulatory potential of MSCs, by means of soluble substances and exosome secretions, could be responsible for the positive effects observed. Further experiments must be done to evaluate if this beneficial effect in vitro also translates to an increase in offspring following embryo transfer. Moreover, this study provides an interesting platform to study the basic requirements during preimplantation embryo development, which, in turn, may aid the improvement of embryo culture protocols in bovine and other species.

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Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media

Cited by 83 publications

References 38 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Improvement of mouse embryo quality by myo-inositol supplementation of IVF media

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

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