Improved in vitro Vitis vinifera L. embryo development of F1 progeny of ‘Delight’ × ‘Ruby seedless’ using putrescine and marker-assisted selection

Li, Tiemei; Li, Zhiqian; Yin, Xiao; Guo, Yurui; Wang, Yuejin; Xu, Yan

doi:10.1007/s11627-018-9895-0

Cited by 17 publications

(10 citation statements)

References 38 publications

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“…The Grape Seedless gene Probe 1 (GSLP1) with an 18-bp sequence can amplify a 569-bp specific band of seedless grapes (Wang and Lamikanra 2002), which has been widely used to analyze the seedless trait . It can amplify the specific band in progeny derived from 'Sultanina', but does not present the band in seeded (Li et al 2015(Li et al , 2018.…”

Section: Introductionmentioning

confidence: 99%

“…The in vitro embryo rescue technique from stenospermocarpic grape has been reported to improve the efficiency of breeding seedless grapes compared with the traditional hybrid technology, and the proportion of seedless progeny obtained from seedless × seedless crosses by embryo rescue is 85% (Cain and Emershad 1983;Agüero et al 1995). Hence, embryo rescue is now widely used for hybridizing seedless grapes (Bouquet et al 1989;Emershad and Ramming 1994;Bouquet and Danglot 1996;Ramming et al 2000;Notsuka et al 2001;Tang et al 2009;Li et al 2015Li et al , 2018.…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that the success of embryo recovery depends on the genotype of the parents , sampling time (Gray et al 1990), and composition of the culture medium (Cain and Emershad 1983;Spiegel-Roy et al 1985;Bouquet et al 1989;Emershad et al 1989). The female and male parents affect both the capacity for embryo formation and the germination of the rescued embryo (Spiegel-Roy et al 1985;Li et al 2015Li et al , 2018Liu et al 2015). The seed trace of different stenospermocarpic grapes can be classified into three types: small, medium, and large.…”

Section: Introductionmentioning

confidence: 99%

“…The two markers are in the promoter region of the AGMOUS-like 11 gene of the V. vinifera L. (VviAGL11), but there is a region (~ 430 bp) between them (Mejía et al 2011). A high linkage disequilibrium of VMC7f2 and p3_VvAGL11 has been shown (Li et al 2018). The SDI locus makes them major candidate markers for the assisted selection of seedlessness and shows a low number of false positives (Bergamini et al 2013;Bennici et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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New disease-resistant, seedless grapes are developed using embryo rescue and molecular markers

Zhao

et al. 2019

3 Biotech

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Improving the operation of embryo rescue technology is a highly efficient way to breed new high-quality cultivars of seedless, disease-resistant grapes when using a stenospermocarpic Vitis vinifera L. (female parents) hybrid with Chinese wild Vitis (male parents). In this study, sampling time had a significant effect on embryo recovery. Four crosses were investigated to improve the embryo rescue efficiency by determining the best sampling time, which was found to be 40, 50, 55, and 57 days after pollination (DAP) for 'Flame seedless' × 'Ruby seedless', 'Kunxiang seedless' × 'Flame seedless', 'Ruby seedless' selfpollination and 'Ruby seedless' × 'Flame seedless', respectively. The highest percentage embryo germination ranged from 60.83 to 89.10% for four cross-combinations when the embryo was germinated on woody plant medium (WPM), with 1.0-μM thidiazuron (TDZ, a potent cytokinin). For 11 cross-combinations, the embryo recovery rate varied from 4.8 to 29.6% and the plant development rate varied from 17.1 to 78.9%. 'Ruby seedless' was the best female parent followed by 'Flame seedless'. The Grape Seedless gene Probe 1 (GSLP1) and SCF27 molecular markers were used to assist the identification of the seedless traits of the 388 progeny. A total of 309 plantlets were amplified, with the specific bands at 569-bp and 2k-bp being preliminarily considered as seedless. The S382-615 marker was used to identify the downy mildew resistance of the 'Ruby seedless' × 'Beichun' cross. Two F1 progeny were amplified, with the specific band at 615-bp being identified as downy mildew resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New disease-resistant, seedless grapes are developed using embryo rescue and molecular markers

Zhao

et al. 2019

3 Biotech

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“…Grapevine (Vitis vinifera L.) is one of the most widely cultivated plants in the world for the production of table grapes (Owens 2008) and wine (Barbalho et al 2020). Seedlessness is a desirable trait in grapevine breeding programs (Varoquaux et al 2000;Ramming et al 2000;Li et al 2018). There are two types of seedless grapevines: parthenocarpy and stenospermocarpy (Stout 1936).…”

Section: Introductionmentioning

confidence: 99%