Improved magnetic lateral flow assays with optimized nanotags for point-of-use inductive biosensing

Salvador, María Lázaro; Gallo-Córdova, Álvaro; Moyano, Amanda; Martínez-García, J.C.; Blanco‐López, M. Carmen; Morales, M.P.; Rivas, M.

doi:10.1039/d0an00849d

Cited by 17 publications

(7 citation statements)

References 45 publications

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“…A quantitative magnetic detection of the test lines was carried out using a home-made inductive sensor specially adapted for scanning LFIA strips [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Moyano

Serrano-Pertierra

Duque

et al. 2021

Sensors

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Colorectal cancer (CRC) is the third leading cause of cancer death and the fourth most common cancer in the world. Colonoscopy is the most sensitive test used for detection of CRC; however, their procedure is invasive and expensive for population mass screening. Currently, the fecal occult blood test has been widely used as a screening tool for CRC but displays low specificity. The lack of rapid and simple methods for mass screening makes the early diagnosis and therapy monitoring difficult. Extracellular vesicles (EVs) have emerged as a novel source of biomarkers due to their contents in proteins and miRNAs. Their detection would not require invasive techniques and could be considered as a liquid biopsy. Specifically, it has been demonstrated that the amount of CD147 expressed in circulating EVs is significant higher for CRC cell lines than for normal colon fibroblast cell lines. Moreover, CD147-containing EVs have been used as a biomarker to monitor response to therapy in patients with CRC. Therefore, this antigen could be used as a non-invasive biomarker for the detection and monitoring of CRC in combination with a Point-of-Care platform as, for example, Lateral Flow Immunoassays (LFIAs). Here, we propose the development of a quantitative lateral flow immunoassay test based on the use of magnetic nanoparticles as labels coupled to inductive sensor for the non-invasive detection of CRC by CD147-positive EVs. The results obtained for quantification of CD147 antigen embedded in EVs isolated from plasma sample have demonstrated that this device could be used as a Point-of-Care tool for CRC screening or therapy monitoring thanks to its rapid response and easy operation.

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“…A quantitative magnetic detection of the test lines was carried out using a home-made inductive sensor specially adapted for scanning LFIA strips [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Moyano

Serrano-Pertierra

Duque

et al. 2021

Sensors

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“…As such, LFIAs have seen extensive global use, for example, in pregnancy tests and notably in the recent COVID-19 pandemic. Since the introduction of this technology in the late 1950s, [38] a wide range of detection labels have been developed for LFIAs, which include colored latex beads, [39,40] colloidal gold nanoparticles, [41,42] magnetic nanoparticles, [43,44] quantum dots, [45,46] enzymes, [47] and fluorophores. [48] However, LFIAs generally suffer from substandard sensitivities when compared to "gold standard" techniques such as PCR.…”

Section: Nanozymes In Point-of-care Devicesmentioning

confidence: 99%

The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

et al. 2023

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Nanozymes represent a class of nanosized materials that exhibit innate catalytic properties similar to biological enzymes. The unique features of these materials have positioned them as promising candidates for applications in clinical sensing devices, specifically those employed at the point‐of‐care. They notably have found use as a means to amplify signals in nanosensor‐based platforms and thereby improve sensor detection limits. Recent developments in the understanding of the fundamental chemistries underpinning these materials have enabled the development of highly effective nanozymes capable of sensing clinically relevant biomarkers at detection limits that compete with “gold‐standard” techniques. However, there remain considerable hurdles that need to be overcome before these nanozyme‐based sensors can be utilized in a platform ready for clinical use. An overview of the current understandings of nanozymes for disease diagnostics and biosensing applications and the unmet challenges that must be considered prior to their translation in clinical diagnostic tests is provided.

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“…In the presence of a magnetic material, the coil acts both as exciting and detecting probe, producing an increase in the impedance proportional to the initial magnetic susceptibility and the number of particles. For monodomain magnetic nanoparticles the susceptibility is maximum at the critical superparamagnetic size (following Néel relaxation model, the initial susceptibility is proportional to the volume as far as the particles are small enough to be superparamagnetic) [51].…”

Section: Biosensing Applicationmentioning

confidence: 99%

Double-Layer Fatty Acid Nanoparticles as a Multiplatform for Diagnostics and Therapy

et al. 2022

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Today, public health is one of the most important challenges in society. Cancer is the leading cause of death, so early diagnosis and localized treatments that minimize side effects are a priority. Magnetic nanoparticles have shown great potential as magnetic resonance imaging contrast agents, detection tags for in vitro biosensing, and mediators of heating in magnetic hyperthermia. One of the critical characteristics of nanoparticles to adjust to the biomedical needs of each application is their polymeric coating. Fatty acid coatings are known to contribute to colloidal stability and good surface crystalline quality. While monolayer coatings make the particles hydrophobic, a fatty acid double-layer renders them hydrophilic, and therefore suitable for use in body fluids. In addition, they provide the particles with functional chemical groups that allow their bioconjugation. This work analyzes three types of self-assembled bilayer fatty acid coatings of superparamagnetic iron oxide nanoparticles: oleic, lauric, and myristic acids. We characterize the particles magnetically and structurally and study their potential for resonance imaging, magnetic hyperthermia, and labeling for biosensing in lateral flow immunoassays. We found that the myristic acid sample reported a large r2 relaxivity, superior to existing iron-based commercial agents. For magnetic hyperthermia, a significant specific absorption rate value was obtained for the oleic sample. Finally, the lauric acid sample showed promising results for nanolabeling.

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Improved magnetic lateral flow assays with optimized nanotags for point-of-use inductive biosensing

Abstract: Magnetic nanotags in lateral flow immunoassay for inductive biosensing: clusters of superparamagnetic critical size optimize the reading out.

Cited by 17 publications

References 45 publications

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

Magnetic Lateral Flow Immunoassay for Small Extracellular Vesicles Quantification: Application to Colorectal Cancer Biomarker Detection

The Nanozyme Revolution: Enhancing the Performance of Medical Biosensing Platforms

Double-Layer Fatty Acid Nanoparticles as a Multiplatform for Diagnostics and Therapy

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