Improved Microtechnique for the Leptospiral Microscopic Agglutination Test

Abstract

A method for improving the original Galton microtechnique for detecting leptospiral antibodies has been developed. Simultaneous titrations were performed on 281 animal and human sera and 17 hyperimmune sera with the microscopic agglutination (MA) test and the improved microtechnique. Reproducibility of the improved microtechnique was determined independently on 65 animal sera by two laboratory sections. The results obtained by comparing positive test data from human and animal sera indicated that agreement between the original MA test and this new method exceeded 94%, whereas the original Galton microtechnique and the original MA test agreed in a maximum of 77% of the tests. This study indicates that the results obtained with the improved microtechnique are much more comparable to results obtained with the original MA test than are those obtained with the original Galton microtechnique.

Serologic Survey of Selected Pathogens in Free-Ranging Bengal Tigers (Panthera tigris tigris) in Nepal

“…1479876) Convention on International Trade in Endangered Species of Wild Fauna and Flora permits, serum samples were transported and evaluated at the New York State Diagnostic Laboratory at Cornell University in Ithaca, New York. Serum samples were evaluated using enzyme-linked immunosorbent assay to detect antibodies to FCoV, FIV, and TOX, and antigens to FeLV (Schaefer et al 2011); serum neutralization to detect antibodies to FHV and CDV (Sheldon et al 2017); hemagglutination inhibition to detect anti-bodies to CPV-2 (Carmichael et al 1980); and a microscopic agglutination test to detect antibodies to Pomona, Hardjo, Ictero, Grippo, Canicola, Autumnalis, and Bratislava serovars of Leptospira interrogans (Cole et al 1973).…”

New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania

“…Isolate RM1 was subjected to serological microagglutination with 42 rabbit sera representative of all pathogenic and four saprophytic serogroups. The rabbit sera were prepared as described by Faine (1982) and the microagglutination test was carried out as described by Cole et al (1973). Monoclonal antibody (mAb) typing was done using a panel of mAbs (F12C3-10, F20C3, F20C4-1, F52C1, F52C2, F70C4-1, F70C7-8, F70C13-1, F70C14-6, F70C20-3, F70C24-14, F70C26-1, F82C1-3, F82C2-2, F82C7-3, F82C8-4, F89C3-3 and F89C12-4) that characteristically agglutinate serovars belonging to serogroups Icterohaemorrhagiae and Sarmin as described by Korver et al (1988).…”

An Improved Immunohistochemical Diagnostic Technique for Canine Leptospirosis using Antileptospiral Antibodies on Renal Tissue

“…17,28 Hematuria and jaundice also may be noted. Serologic tests include the microscopic agglutination test 10 and the enzyme-linked immunosorbent assay. 23 Fluorescent antibody tests, 9 dark field microscopy, culture, polymerase chain reaction (PCR) assays, and histopathology with special stains (e.g., silver) can be used to identify leptospires in the tissues or body fluid of dogs.…”