Improved production of β-glucan by a T-DNA–based mutant of Aureobasidium pullulans

Chen, Xing; Wang, Ying; He, Congrong; Wang, Guoliang; Zhang, Gaochuan; Wang, Chonglong; Wang, Dahui; Zou, Xiang; Wei, Gongyuan

doi:10.1007/s00253-021-11538-x

Cited by 8 publications

(4 citation statements)

References 59 publications

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“…The genus Aureobasidium is popularly known as black yeast due to its melanin production, [1] and can be used in the production of many natural products such as pullulan, [2] β-glucan, [3] Lpiperazic acid, [4] liamocin, [5] poly(β-L-malic acid) (PMLA), [6] and melanin. Chemical investigation on marine-derived Aureobasidium led to the isolation of aureobasidin, an ester with an unusual 4,6-dihydroxydecanoic acid residue; cyclo(D-cis-Hyp-L-Phe) and cyclo(D-cis-Hyp-L-Leu), two diketopiperazines with a D-cis-4-hydroxyproline residue; and orcinotriol, a 1,3-dihydroxyphenol derivative.…”

Section: Introductionmentioning

confidence: 99%

“…[10,11] As our current research interest focused on the bioactive secondary metabolites with novel structures from deep-seaderived fungi, [12][13][14][15] the fungus strain A. melanogenum LUO5 collected from the deep-sea derived at a depth of 8225 m in the North Pacific was chosen for a systemic chemical investigation due to its potential anti-inflammation activity in LPSinduced BV-2 cells. As a result, three new C 10 and C 12 aliphatic δ-lactones (1)(2)(3), three new fatty acid methyl esters (4)(5)(6), together with eight known compounds (7)(8)(9)(10)(11)(12)(13)(14) were obtained (Figure 1). Herein, we report the isolation, structure, and bioactivities of these isolates.…”

Section: Introductionmentioning

confidence: 99%

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Chemical Constituents from the deep‐sea‐derived Fungus Aureobasidium melanogenum LUO5

Dong,

Wang,

Zhang

et al. 2024

Chemistry & Biodiversity

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Three new C10 and C12 aliphatic δ‐lactones (1–3), three new fatty acid methyl esters (4–6), and eight known compounds (7–14) were isolated from the marine Aureobasidium sp. LUO5. Their structures were established by detailed analyses of the NMR, HRESIMS, optical rotation, and ECD data. All isolates were tested for their inhibitory effects on nitric oxide production in LPS‐induced BV‐2 cells. Notably, compound 4 displayed the strongest inhibitory effect with the IC50 value of 120.3 nM.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemical Constituents from the deep‐sea‐derived Fungus Aureobasidium melanogenum LUO5

Dong,

Wang,

Zhang

et al. 2024

Chemistry & Biodiversity

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“…Pullulan produced by A. pullulans fermentation normally contains byproducts, including melanin, glucan and polymalic acid ( Zeng et al, 2020 ; Chen et al, 2021 ; Liu et al, 2021 ). The presence of these impurities largely increases the difficulty of pullulan purification.…”

Section: Introductionmentioning

confidence: 99%

High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06

Chen

Zheng

Gao

et al. 2023

Front. Bioeng. Biotechnol.

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Pullulan has many potential applications in the food, pharmaceutical, cosmetic and environmental industries. However, the yield and molecular properties of pullulan produced by various strains still need to be promoted to fit the application needs. A novel yeast-like strain Aureobasidium pullulans BL06 producing high molecular weight (Mw) pullulan (3.3 × 106 Da) was isolated and identified in this study. The remarkable Mw of pullulan produced by A. pullulans BL06 was the highest level ever reported thus far. To further regulate the biosynthesis of pullulan in A. pullulans BL06, three gene knockout strains A. pullulans BL06 ΔPMAs, A. pullulans BL06 Δmel, and A. pullulans BL06 ΔPMAsΔmel, were constructed. The results showed that A. pullulans BL06 ΔPMAs could produce 140.2 g/L of moderate Mw (1.3 × 105 Da) pullulan after 120 h of fermentation. The highest yield level of pullulan to date could vastly reduce its production cost and expand its application scope and potential. The application experiments in food preservation showed that the moderate-Mw pullulan obtained in this work could reduce the weight loss of celery cabbages and mangos by 12.5% and 22%, respectively. Thus, the novel strains A. pullulans BL06 and A. pullulans BL06 ΔPMAs possessed unlimited development prospects in pullulan production at various Mw ranges and pullulan applications in multiple fields.

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“…Many novel pathogenicity-related genes have been discovered through large-scale screening of T-DNA mutant libraries of phytopathogenic fungi, such as Verticillium dahliae , Ustilaginoidea virens , and Sporisorium scitamineum [ 20 , 21 , 22 ]. In the industrial cultivation of filamentous fungi, the ATMT method has been proven an efficient tool for screening random T-DNA insertion mutants to identify those with improved production of certain metabolic products [ 23 , 24 ]. In the current study, we performed a large-scale screen of T-DNA tagged mutants of C. militaris and found that the mutation of a gene encoding a fungal-specific transcription factor resulted in a white colony phenotype upon exposure to light.…”

Section: Introductionmentioning

confidence: 99%

Cmcrf1, a Putative Zn2Cys6 Fungal Transcription Factor, Is Involved in Conidiation, Carotenoid Production, and Fruiting Body Development in Cordyceps militaris

Zhang

Lan

et al. 2022

Biology

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Cordyceps militaris is a high-value medicinal and edible fungus that produces many bioactive compounds, including carotenoid, and thus, improving the carotenoid productivity of C. militaris will increase its commercial value. However, little is known about the genetic regulatory mechanism of carotenoid biosynthesis in C. militaris. To further understanding the regulatory mechanism of carotenoid biosynthesis, we performed a large-scale screen of T-DNA insertional mutant library and identified a defective mutant, denoted T111, whose colonies did not change color from white to yellow upon exposure to light. Mutation analysis confirmed that a single T-DNA insertion occurred in the gene encoding a 695-amino-acid putative fungal-specific transcription factor with a predicted Zn2Cys6 binuclear cluster DNA-binding domain found uniquely in fungi. Targeted deletion of this gene, denoted C. militaris carotenogenesis regulatory factor 1 (Cmcrf1), generated the ΔCmcrf1 mutant that exhibited drastically reduced carotenoid biosynthesis and failed to generate fruiting bodies. In addition, the ΔCmcrf1 mutant showed significantly increased conidiation and increased hypersensitivity to cell-wall-perturbing agents compared with the wild-type strain. However, the Cmcrf1 gene did not have an impact on the mycelia growth of C. militaris. These results show that Cmcrf1 is involved in carotenoid biosynthesis and is required for conidiation and fruiting body formation in C. militaris.

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Improved production of β-glucan by a T-DNA–based mutant of Aureobasidium pullulans

Cited by 8 publications

References 59 publications

Chemical Constituents from the deep‐sea‐derived Fungus Aureobasidium melanogenum LUO5

Chemical Constituents from the deep‐sea‐derived Fungus Aureobasidium melanogenum LUO5

High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06

Cmcrf1, a Putative Zn2Cys6 Fungal Transcription Factor, Is Involved in Conidiation, Carotenoid Production, and Fruiting Body Development in Cordyceps militaris

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