Improved purification procedure for rabbit muscle amylo-1,6-glucosidase/Oligo-1,4 → 1,4-glucantransferase

Cystic Fibrosis: Enzymatic Detection of a Ciliostatic Factor

“…This modification allowed use of higher concentrations of inhibitors. Mammalian debranching en/ymc from rabbit muscle (amylo-1,6-glucosidase/4-cu-glucanotr;1nsfcras) was purified as described previously (27). Its activity was measured using glycogen phosphorylase limit dextrin as a substrate in the static assay described by Nelson, Kolb, and Larner (23).…”

“…The effect of the heparin lyophilized dialysate on debranching enzyme was determined by addition of 10 to 100 p1 of the dialysate solution to a total of 200 pl in the standard limit dextrin static assay (27). Controls run simultaneously contained 10-100 pl H,O in place of the dialysate solution.…”

Specificity of an Isolated Salivary Factor Material to Cystic Fibrosis

“…Purified rabbit muscle glycogen debranching enzyme (amylo-1,6-glucosidase:4-a-glucanotransferase) was prepared according to the method of Watts and Nelson (35). Glycogen phosphorylase limit dextrin was prepared as described previously (29).…”

Cystic Fibrosis: Isolation and Physical Properties of a Salivary Cystic Fibrosis Factor

“…Purified rabbit muscle glycogen debranching enzyme (amylo-1,6-glucosidase/4-a-glucanotransferase) was prepared according to the method of Watts and Nelson (42). Glycogen phosphorylase limit dextrin was prepared as described previously (37).…”

“…Glycogen phosphorylase limit dextrin was prepared as described previously (37). Additional materials for the debrancher assay were obtained as described previously (42). Soluble potato starch used for routine a-amylase assays was obtained from Merck and Co., Rahway, NJ.…”