Improved rapid autoradiography with elimination of background

Spiridonidis, Charalampos; Raza, Azra; Zhao, Shirley; Preisler, Harvey D.

doi:10.1002/cbf.290030211

Cited by 2 publications

(1 citation statement)

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“…FUdr was simultaneously added to the suspension because of its potent inhibitory eeffect on the enzyme thymidylate synthetase [U]. By reducing the endogenous formation of thymidylates from uridylate precursors, FUdr increases the utilization of exogenously provided tritiated thymidine (3HTdr), and the quality of the autoradiographic method can be significantly improved [15]. Cells were then washed thrice in unlabeled thymidine and once in phosphatebuffered saline (PBS).…”

Section: Sample Processingmentioning

confidence: 99%

Analysis of cell cycle characteristics and course of the disease in ANLL

et al. 1987

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The cell cycle characteristics of a newly diagnosed patient with acute nonlymphocytic leukemia (ANLL) were studied by using intravenous bromodeoxyuridine (BrdU) and our previously described "double-label" technique. The percentage of S-phase cells in the bone marrow (BM) biopsy were 25% compared to 7% from the simultaneously obtained BM aspirate. The duration of S-phase (Ts) was determined to be 4 hr and the total cell cycle time (Tc) was 16 hr. We demonstrated that the actual clinical course of this patient's illness corresponded well with the course predicted on the basis of these cell cycle measurements. Although he achieved aplasia in response to two successive courses of induction chemotherapy, leukemic cells repopulated the marrow, producing a rapidly rising PB white blood cell (WBC) count with a T1/2 of approximately 20 hr each time. It is likely that the resistance of this patient's leukemia to therapy was a result of the rapid proliferative rate of his leukemic cells and not due to the inability of chemotherapeutic agents to kill a large number of cells. Since the measurements can be completed within 48 hr, measuring Ts and Tc will provide a better understanding of the biological differences that exist between patients with ANLL.

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Section: Sample Processingmentioning

confidence: 99%