Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution

Bessems, Maud; Doorschodt, Benedict M.; Vliet, Arlène K. van; Gulik, Thomas M. van

doi:10.1002/lt.20388

Cited by 75 publications

(46 citation statements)

References 42 publications

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“…5 and Table 6 simulate most closely the conditions of continuous hypothermic perfusion. The optimal preservation solutions for effective machine perfusion have not yet been established and modifications have recently been proposed [32][33][34][35][36]. Based on our data, we would suggest to include an iron chelator into these solutions and, in addition, to replace histidine by less toxic derivatives such as Nα-acetyl-L-histidine.…”

Section: Discussionmentioning

confidence: 85%

Histidine-induced injury to cultured liver cells, effects of histidine derivatives and of iron chelators

Rauen

Klempt

Groot

2006

Cell. Mol. Life Sci.

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The amino acid histidine is an excellent buffer and is therefore included in several organ preservation solutions used in transplantation medicine. However, when used at concentrations as in these solutions, histidine has a marked injurious potential. Therefore, we here assessed the mechanism of histidine-induced cell injury and searched for ways to use the buffering power of histidine but avoid histidine toxicity. When cultured hepatocytes were incubated in HTK solution or in modified Krebs-Henseleit buffer containing 198 mM L-histidine at 37 degrees C, most cells lost viability within 3 h (LDH release 86 +/- 7% and 89 +/- 5%, respectively). This injury was accompanied by marked lipid peroxidation, and was strongly inhibited by hypoxia, by the antioxidants trolox, butylated hydroxytoluene and N-acetylcysteine and by the membrane-permeable iron chelators 2,2'-dipyridyl, 1,10-phenanthroline, LK 614, LK 616 and deferoxamine. Thus, histidine-induced cell injury appears to be mediated by an iron-dependent formation of reactive oxygen species. D-Histidine, imidazol and L-histidine methyl ester also elicited marked injury, while the N-substituted derivatives Nalpha-acetyl-L-histidine and tert-butyl-oxycarbonylhistidine and histidine-containing dipeptides showed almost no toxicity. Histidine toxicity, its iron dependence and the superiority of Nalpha-acetyl-L-histidine were also evident during/after cold (4 degrees C) incubations. Therefore, we suggest the addition of iron chelators to histidine-containing solutions, and/or replacing histidine with Nalpha-acetyl-L-histidine in organ preservation solutions.

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Section: Discussionmentioning

confidence: 85%

Histidine-induced injury to cultured liver cells, effects of histidine derivatives and of iron chelators

Rauen

Klempt

Groot

2006

Cell. Mol. Life Sci.

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“…Its composition is based on the fact that metabolism is still present at 4°C. Polysol has been tested both as an experimental CS solution and as HMP solution (93,94). It is a classic preservation solution enriched with amino acids, vitamins, and antioxidants (95).…”

Section: New Solutionsmentioning

confidence: 99%

Perspectives in Organ Preservation

2007

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Maintaining organ viability after donation until transplantation is critically important for optimal graft function and survival. To date, static cold storage is the most widely used form of preservation in every day clinical practice. Although simple and effective, it is questionable whether this method is able to prevent deterioration of organ quality in the present era with increasing numbers of organs retrieved from older, more marginal, and even non-heart-beating donors. This review describes principles involved in effective preservation and focuses on some basic components and methods of abdominal organ preservation in clinical and experimental transplantation. Concepts and developments to reduce ischemia related injury are discussed, including hypothermic machine perfusion. Despite the fact that hypothermic machine perfusion might be superior to static cold storage preservation, organs are still exposed to hypothermia induced damage. Therefore, recently some groups have pointed at the beneficial effects of normothermic machine perfusion as a new perspective in organ preservation and transplantation.

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“…POLYSOL has demonstrated favorable results after machine perfusion and cold static storage preservation for liver [14], small bowel [15], and porcine kidney autotransplantation [16]. Among its 65 components, the most important components in POLYSOL are polyethylene glycol (PEG), antioxidant, vitamins, and adenosine for improved energy and fat metabolism in the liver allograft.…”

Section: Discussionmentioning

confidence: 98%

Improved Preservation and Microcirculation with POLYSOL After Partial Liver Transplantation in Rats

Yagi

Doorschodt

Afify

et al. 2011

Journal of Surgical Research

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Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution

Cited by 75 publications

References 42 publications

Histidine-induced injury to cultured liver cells, effects of histidine derivatives and of iron chelators

Histidine-induced injury to cultured liver cells, effects of histidine derivatives and of iron chelators

Perspectives in Organ Preservation

Improved Preservation and Microcirculation with POLYSOL After Partial Liver Transplantation in Rats

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