Improved resolution in high-sensitivity dual-label gas radiochromatography

Thomas, Paul J.; Dutton, H. J.

doi:10.1021/ac60273a012

Cited by 15 publications

(3 citation statements)

References 14 publications

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“…Dual-labeled samples were analyzed on an Aerograph model A-90P gas chromatograph modified to provide for serial collection of the gas chromatographic column effluent in scintillation solvent (17). The radiochemical fractions collected were counted in a Beckman LS-250 liquid scintillation counter hardware wired to an IBM 1800 computer programed to calculate the disintegrations/rain of the 14 C and 3H isotopes and the composition of 14C and 3H esters.…”

Section: Discussionmentioning

confidence: 99%

Competitive hydrogenation rates of isomeric methyl octadecadienoates

Snyder¹,

Scholfield²,

Mounts³

et al. 1975

J Americ Oil Chem Soc

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Determination of the relative reaction rates of isomeric methyl octadecadienoates is possible by competitive reduction of a mixture containing an inactive diene and a radioactively labeled isomer. The hydrogenation rate of methyl cis-9,cis-12-octadecadienoate with platinum and nickel catalysts is compared to the hydrogenation rate of each of several isomers of methyl octadecadienoate, and the relative rate of the competitive hydrogenations is calculated by a digital computer. Methyl cis-9,cis-12 Iinoleate is reduced the most rapidly of all the dienes studied. The relative rates of the positional isomers tend to decrease with the increasing number of methylene groups between the double bonds, except when one of the double bonds is in the more reactive 15 position. Comparison of the geometric isomers shows that trans, trans diene is hydrogenated at a slower rate than cis, cis linoleate.

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Section: Discussionmentioning

confidence: 99%

Competitive hydrogenation rates of isomeric methyl octadecadienoates

Snyder¹,

Scholfield²,

Mounts³

et al. 1975

J Americ Oil Chem Soc

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“…Analysis of radioactive fatty acids. Labeled methyl esters of the fatty acids were analyzed with a gas chromatography-liquid scintillation spectrometer system (5,21). An Aerograph A-90 gas chromatograph equipped with a thermal conductivity detector was modified to provide a union of the effluent currents from both the sample and reference cells.…”

Section: Methodsmentioning

confidence: 99%

Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis

Nickerson¹,

Bulla²,

Mounts³

1975

J Bacteriol

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The biosynthesis of individual branchedand normal-chain fatty acids during Bacillus thuringiensis spore germination and outgrowth was studied by comparing pulsed and continuous labeling of these fatty acids with [U-14C ]acetate. The relative specific activity of each fatty acid varies with time as the cell progresses through outgrowth. However, fatty acid synthesis does occur in two distinct phases. Upon germination, acetate is incorporated only into the iso-isomers i-C18,, i-C14, and i-C1*; no normal or anteiso synthesis occurs. Subsequent to T8,s the full complement of branchedand normal-chain homologues is formed and there is a dramatic enhancement in the overall rate of fatty acid synthesis. Significantly, this rate increase coincides with a marked shift from the synthesis of short-chain to long-chain fatty acids. These findings illustrate a dichotomy in synthesis that may result from initial fatty acid formation by preexisting spore fatty acid biosynthetic enzymes in the absence of de novo protein synthesis. Elucidation of the timing and kinetics of individual fatty acid formation provides a biochemical profile of activities directly related to membrane differentiation and cellular development.

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“…Because the radioactive precursors were added in low concentration without accompanying carrier, the overall fatty acid composition of the cells remained unchanged. Subsequently, the fatty acids were extracted and converted to their methyl esters, and the extent of precursor incorporation into specific fatty acids was determined on a gas chromatograph interfaced to a liquid scintillation counter (10,14). Efficiency of recovery of radioactively labeled fatty acids was about 75% using this technique.…”

mentioning

confidence: 99%

Incorporation of Specific Fatty Acid Precursors During Spore Germination and Outgrowth in Bacillus thuringiensis

Nickerson

Bulla²

1980

Appl Environ Microbiol

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The selective incorporation of precursors specific for individual fatty acids in germinating and outgrowing spores of Bacillus thuringiensis is described. The specific precursors utilized were [ 14 C]butyrate, -isobutyrate, -valerate, and -isovalerate, which were incorporated into even-numbered normal-chain isomers, even-numbered iso-isomers, odd-numbered normal-chain acids, and odd-numbered isohomologs, respectively. This preferential incorporation by B. thuringiensis allows the terminal carbons of specific normal and branched-chain fatty acids, contained within the cytoplasmic membrane, to be labeled with 14 C and, potentially, 13 C.

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Improved resolution in high-sensitivity dual-label gas radiochromatography

Cited by 15 publications

References 14 publications

Competitive hydrogenation rates of isomeric methyl octadecadienoates

Competitive hydrogenation rates of isomeric methyl octadecadienoates

Lipid metabolism during bacterial growth, sporulation, and germination: differential synthesis of individual branched- and normal-chain fatty acids during spore germination and outgrowth of Bacillus thuringiensis

Incorporation of Specific Fatty Acid Precursors During Spore Germination and Outgrowth in Bacillus thuringiensis

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