Improved sample preparation for the testosterone hydroxylation assay using disposable extraction columns

Oesch, Franz; Wagner, H.; Platt, Karl L.; Arand, Michael

doi:10.1016/0378-4347(92)80324-j

Cited by 21 publications

(4 citation statements)

References 9 publications

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“…These compounds are not only metabolized by CYPlAl but they are also capable 1 of inducing the activity of the enzyme. Apart from xenobiotic metabolism, CYPl A1 can participate in the inactivation of testosterone (Oesch et al, 1992) and, therefore, an increased enzyme activity could affect testicular function. With regard to male reproductive function, it has been hypothesized that oxidative stress of spermatozoa is an important factor of infertility (Aitken, 1994).…”

Section: Discussionmentioning

confidence: 99%

Increased frequencies of cytochrome P4501A1 polymorphisms in infertile men

et al. 2009

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Summary. Genetic factors that could mediate the pathogenesis of male reproductive disorders are largely unclear. Polymorphisms of cytochrome P4501A1 (CYP1A1), a key enzyme in the extrahepatic metabolism of lipophilic xenobiotics, have been shown to influence susceptibility to xenobiotics. Here, CYP1A1 polymorphisms were investigated in 134 infertile Caucasian men. The frequencies of the Mspl polymorphism in the 3′‐flanking region of the CYP1A1 gene and a mutation in exon 7 causing an isoleucine‐valine exchange (IVE) in the heme‐binding region of the enzyme were increased among infertile men when compared with those of unselected, healthy male controls (odds ratio (OR)) 1.4, Cl95 0.68–2.89 for Mspl polymorphism; OR 2.4, Cl95 0.83–6.95 for IVE). Patients with normozoospermia revealed the highest frequencies for both polymorphisms (n = 8; OR 4.5, Cl95 0.97–20.91 for Mspl polymorphism; OR 13.7, Cl95 2.53–74.13 for IVE). ORs for the IVE exceeded the values calculated for the Mspl polymorphism. These preliminary results suggest that genetic variation in the metabolism of xenobiotics may codetermine individual susceptibility to infertility.

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Section: Discussionmentioning

confidence: 99%

Increased frequencies of cytochrome P4501A1 polymorphisms in infertile men

et al. 2009

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“…7-Ethoxycoumarin and 7-ethoxyresorufin dealkylase (ECOD, EROD), aldrin epoxidase (ALD), aromatase, epoxide hydrolase (EH), glutathione-S-transferase (GS-T), UDP-glucuronyltransferase (GLU-T) and in vitro testosterone hydroxylation were determined using published methods (Ullrich and Weber, 1972;Burke et al, 1977;Wolff et al, 1979;Biegel et al, 1995;Guiliano et al, 1980;Habig et al, 1974;Bock, 1974;Oesch et al, 1992).…”

Section: Determination Of Liver Enzyme Activitiesmentioning

confidence: 99%

Evaluation of the rodent Hershberger bioassay: Testing of coded chemicals and supplementary molecular-biological and biochemical investigations

2007

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“…Where the extra‐hepatic metabolism of compounds such as polycyclic aromatic hydrocarbons (PAHs) or heterocyclic amines is concerned, it is mainly CYP1A1 that has to be considered ( Guengerich, 1992; Nebert et al ., 1996 ). Notably, the broad and overlapping substrate specificities of xenobiotic‐metabolizing CYPs also comprise endogenous molecules including steroid hormones ( Oesch et al ., 1992 ; Yager, 1996).…”

Section: Xenobiotic‐metabolizing Enzymesmentioning

confidence: 99%

Xenobiotic metabolism, genetic polymorphisms and male infertility

et al. 2000

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Male reproductive function may be impaired by various occupational and environmental chemical agents. The majority of these xenobiotics, however, require metabolic activation in order to exert adverse effects via covalent interactions between intermediate metabolites and cellular macromolecules such as DNA or protein. In addition, metabolization may alter endocrine-disrupting properties of xenobiotics. Thus tissue-specific expression and regulation of multiple xenobiotic-metabolizing enzymes are likely to play an important role in chemically induced disorders of male reproductive organs. Recent studies suggest that genetic polymorphisms underlying inter-individual and inter-ethnic variability of xenobiotic metabolism modulate susceptibility to male reproductive disorders. For cytochrome P450 1A1 (CYP1A1), a key enzyme in extra-hepatic metabolic activation of lipophilic xenobiotics, increased frequencies of two genetically linked polymorphisms have been found among infertile men.

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Improved sample preparation for the testosterone hydroxylation assay using disposable extraction columns

Cited by 21 publications

References 9 publications

Increased frequencies of cytochrome P4501A1 polymorphisms in infertile men

Increased frequencies of cytochrome P4501A1 polymorphisms in infertile men

Evaluation of the rodent Hershberger bioassay: Testing of coded chemicals and supplementary molecular-biological and biochemical investigations

Xenobiotic metabolism, genetic polymorphisms and male infertility

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