Clinical Chemistry

1981

DOI: 10.1093/clinchem/27.1.198a

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Improved sample treatment before liquid-chromatographic determination of anticonvulsants in serum.

Renjith George¹

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Cited by 14 publications

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Drug Analyses in a Department of Forensic Medicine and Toxicology

1986

Analytical Methods in Human Toxicology

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Drug Analyses in a Department of Forensic Medicine and Toxicology

1986

Analytical Methods in Human Toxicology

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Rapid isolation of some antiepileptic hydantoins and their analogues with Sep-Pak C18 cartridges and capillary gas chromatography with splitless injection

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et al. 1990

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A simple and rapid method for isolation of seven antiepileptics (2 hydantoin, 2 oxazolidin, and 3 suximide derivatives) from urine and plasma is presented. Urine and plasma (1 ml) samples containing seven antiepileptics were mixed with distilled water (4 ml), and the sample solution was poured into a pretreated Sep-Pak C18 cartridge; this was washed with water and chloroform/methanol was passed through it to elute the antiepileptics. The eluate was mixed with isoamyl acetate and evaporated under a stream of N2. The drugs were detected by gas chromatography with fused silica capillary columns, splitless injection and flame ionization detection. Separation of the seven antiepileptics from each other and from impurities was satisfactory with the use of an SPB-1 capillary column. The detection limit for the seven antiepileptics with the present method was 0.1-1.0 microgram/ml urine or plasma. The recovery of the drugs from urine and plasma was more than 70% and 50%, respectively.

Electrolytes and nitrogen compounds of body fluids and tissues ofOctopus vulgaris Lam.

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et al. 1986

J Comp Physiol B

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