Improved selectivity in detection of polar basic drugs by liquid chromatography–electrospray ionization mass spectrometry

Campanero, Miguel Ángel; Bueno, I.; Arangoa, M. A.; Escolar, M.; Quetglas, Emilio García; López-Ocáriz, A.; Azanza, J. R.

doi:10.1016/s0378-4347(01)00355-3

Cited by 32 publications

(33 citation statements)

References 14 publications

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“…A number of analytical methods have been reported for the determination of individual H 2 antagonists in human plasma since 1990 years, cimetidine [1][2][3][4][5][6][7][8], ranitidine [9][10][11][12][13][14][15][16][17], famotidine [18][19][20][21][22][23][24][25], and lafutidine [26][27][28][29]. Apart from some uncommonly used methods such as free capillary zone electrophoresis [4], micellar electrokinetic capillary chromatography [7], HPTLC [13], most reports utilized HPLC-UV method [1][2][3][4][5][6][7][9][10][11][12][13][14][15][16][18][19]…”

Section: Introductionmentioning

confidence: 99%

“…Apart from some uncommonly used methods such as free capillary zone electrophoresis [4], micellar electrokinetic capillary chromatography [7], HPTLC [13], most reports utilized HPLC-UV method [1][2][3][4][5][6][7][9][10][11][12][13][14][15][16][18][19][20][21][22][23]26,27]. Although HPLC method is mature and relatively economical, limitations such as sensitivity, selectivity and throughput were often encountered during the method development.…”

Section: Introductionmentioning

confidence: 99%

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A single LC–tandem mass spectrometry method for the simultaneous determination of four H2 antagonists in human plasma

Sun

Tian

Zhang

et al. 2009

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A single LC–tandem mass spectrometry method for the simultaneous determination of four H2 antagonists in human plasma

Sun

Tian

Zhang

et al. 2009

Journal of Chromatography B

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“…The method described here is, in contrast, readily accomplished using one HPLC pump working under isocratic conditions. Also, the tandem MS detection as used by Campanero et al [16], which seems to be the method of choice, is still not universally available.…”

Section: Precision Accuracy Lloqmentioning

confidence: 99%

“…However, these papers have essentially been modifications of the original method of Vincek [3]. Various efforts to improve it by adopting more sophisticated approaches have been published in recent years [11,16]. The new procedure outlined here (with a LiChrospher column) has been successfully used for determination of famotidine in human plasma in a bioequivalence study [13] with famotidine concentrations in plasma ranging from 5 to 175 ng mL -1 , thus documenting the applicability of this method in routine analysis.…”

Section: Precision Accuracy Lloqmentioning

confidence: 99%

Selective determination of famotidine in human plasma by high performance liquid chromatography in alkaline media with solid phase extraction

Anzenbacherová¹,

Filipová

Nobilis

et al. 2003

J of Separation Science

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Selective determination of famotidine in human plasma by high performance liquid chromatography in alkaline media with solid phase extraction A new method is described for the determination of famotidine by solid phase extraction from alkalinized human plasma followed by reversed phase (RP) HPLC in acetonitrile/alkaline buffer with molsidomine as an internal standard. Different acetonitrile/ aqueous buffer mobile phases as well as various RP columns were used. Alkaline medium allowed the limit of quantitation to be lowered to 5 ng/mL of plasma as the famotidine gives more intense absorption at about 286 nm (at pH values higher than 7). Moreover, work in alkaline media and at this wavelength is highly selective as peaks corresponding to impurities present in most samples are well separated. A method using a mildly alkaline mobile phase (acetonitrile/10 mM phosphate with 10 mM 1-heptanesulphonic acid, pH 7.5) was successfully used for determination of famotidine in human plasma in a pharmacokinetic study.

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“…5 Na literatura encontram-se inúmeros trabalhos referentes à determinação de famotidina em formulações farmacêuticas. Dentre os métodos descritos estão a eletroforese capilar com detecção no UV, [6][7][8][9] potenciometria, 10 voltametria, 11 espectrofotometria, 12-17 cromatografia líquida de alta eficiência (CLAE) [18][19][20][21][22][23][24][25][26][27][28][29][30][31] e análise por injeção em fluxo. [32][33][34] As Farmacopeias Brasileira e Americana registram a monografia para famotidina na forma farmacêutica comprimido com quantificação por CLAE.…”

Section: Introductionunclassified

Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas

Favoretto¹,

Souza²,

Bonfílio³

et al. 2010

Quím. Nova

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Recebido em 21/10/09; aceito em 22/3/10; publicado na web em 18/6/10 VALIDATION OF AN UV SPECTROPHOTOMETRIC METHOD FOR QUANTIFICATION OF FAMOTIDINE IN CAPSULES.This work describes the validation of a method for quantification of famotidine in capsules by UV spectrophotometry using as solvents buffer solution at pH 2.5 and buffer solution at pH 4.5. The results show that the method is practical, selective, accurate, precise and linear from 10.0 to 25.0 mg/mL of famotidine in both solvents. However, a variance analysis showed a lower mean percentage of famotidine when buffer at pH 2.5 is used. This effect was attributed to the instability of famotidine in acidic media. Therefore, the use of pH 4.5 buffer was considered suitable for analysis of famotidine in capsules.Keywords: famotidine; validation; UV spectrophotometry. INTRODUÇÃOA famotidina (Figura 1) é um antagonista competitivo do receptor H 2 da histamina. Seu mecanismo de ação consiste na inibição da secreção ácida gástrica, evitando que o seu excesso possa causar dor e mal estar. Este fármaco é utilizado no tratamento de úlceras pépticas e da hiperacidez gástrica.1-3 Os bloqueadores dos receptores H 2 , como a famotidina, reduzem eficazmente a secreção ácida gástrica estimulada por alimentos, pentagastrina, insulina, cafeína ou histamina. Esta diminuição está relacionada com a redução tanto do conteúdo gástrico, quanto do volume do suco gástrico, e ocorre em indivíduos sadios e com úlceras pépticas. A famotidina é bem absorvida por via oral e atinge níveis terapêuticos em cerca de 1 h. É eliminada principalmente por via renal, sendo também excretada no leite materno.1,4 São raros os efeitos indesejados e, em geral, são revertidos com a interrupção do tratamento.2 Pode ter sua ação diminuída por antiácidos e sucralfato, porém pode diminuir a ação do cetoconazol e itraconazol. Também pode sofrer ou provocar aumento das reações adversas com depressores da medula óssea. 5Na literatura encontram-se inúmeros trabalhos referentes à determinação de famotidina em formulações farmacêuticas. Dentre os métodos descritos estão a eletroforese capilar com detecção no UV, [6][7][8][9] potenciometria, 10 voltametria, 11 espectrofotometria, 12-17 cromatografia líquida de alta eficiência (CLAE) 18-31 e análise por injeção em fluxo. 32-34As Farmacopeias Brasileira e Americana registram a monografia para famotidina na forma farmacêutica comprimido com quantificação por CLAE. 35,36 No entanto, para identificação do fármaco é utilizada a varredura espectral com solução de tampão fosfato pH 2,5, 35 e, ainda, no ensaio de dissolução para comprimidos a quantidade de fármaco liberado da forma farmacêutica é quantificada por espectrofotometria utilizando-se solução de tampão fosfato pH 4,5. 36A forma farmacêutica cápsulas contendo famotidina não se encontra inscrita, até o presente momento, em compêndios oficiais e, dentre os medicamentos manipulados em farmácias, este fármaco tem se destacado para o atendimento de pacientes com transtornos gástricos. Tendo em vista o grande número...

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Improved selectivity in detection of polar basic drugs by liquid chromatography–electrospray ionization mass spectrometry

Cited by 32 publications

References 14 publications

A single LC–tandem mass spectrometry method for the simultaneous determination of four H2 antagonists in human plasma

A single LC–tandem mass spectrometry method for the simultaneous determination of four H2 antagonists in human plasma

Selective determination of famotidine in human plasma by high performance liquid chromatography in alkaline media with solid phase extraction

Validação de método espectrofotométrico na região do UV para quantificação de famotidina em cápsulas

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