Improved sensitivity, accuracy and prediction provided by a high‐performance liquid chromatography screen for the isolation of phytase‐harbouring organisms from environmental samples

Rix, Gregory D.; Todd, Jonathan D.; Neal, Andrew L.; Brearley, Charles A.

doi:10.1111/1751-7915.13733

Cited by 6 publications

(6 citation statements)

References 66 publications

(94 reference statements)

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“…In addition to BPPs, the genes and the phytase activity related to histidine acid phosphatases and PAPs were found in Acinetobacter and Buttiauxella sp. of soil samples (Rix et al ., 2020 ).…”

Section: Diversity and Selectivity Of Phosphatases For P O ...mentioning

confidence: 99%

Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation

Park

Solhtalab

Thongsomboon

et al. 2022

Environ Microbiol Rep

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Summary Critical to meeting cellular phosphorus (P) demand, soil bacteria deploy a number of strategies to overcome limitation in inorganic P (Pi) in soils. As a significant contributor to P recycling, soil bacteria secrete extracellular enzymes to degrade organic P (Po) in soils into the readily bioavailable Pi. In addition, several Po compounds can be transported directly via specific transporters and subsequently enter intracellular metabolic pathways. In this review, we highlight the strategies that soil bacteria employ to recycle Po from the soil environment. We discuss the diversity of extracellular phosphatases in soils, the selectivity of these enzymes towards various Po biomolecules and the influence of the soil environmental conditions on the enzyme's activities. Moreover, we outline the intracellular metabolic pathways for Po biosynthesis and transporter‐assisted Po and Pi uptake at different Pi availabilities. We further highlight the regulatory mechanisms that govern the production of phosphatases, the expression of Po transporters and the key metabolic changes in P metabolism in response to environmental Pi availability. Due to the depletion of natural resources for Pi, we propose future studies needed to leverage bacteria‐mediated P recycling from the large pools of Po in soils or organic wastes to benefit agricultural productivity.

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Section: Diversity and Selectivity Of Phosphatases For P O ...mentioning

confidence: 99%

Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation

Park

Solhtalab

Thongsomboon

et al. 2022

Environ Microbiol Rep

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“…Phytase ( myo -inositol hexakisphosphate phosphohydrolase) produces P by hydrolyzing phytate ( Lei et al, 2013 ). Many bacteria have been reported to secrete phytases, such as Pseudomonas , Bacillus , Achromobacter , Acinetobacter , Klebsiella , Micrococcus , Burkholderia , and Serratia ( Jorquera et al, 2018 ; Neal et al, 2018 ; Wan et al, 2020 ; Rix et al, 2021 ), which are isolated in different habitats, and support its great potential as a suitable phytase producer with desired properties. Three groups of phytase encoding β-propeller phytases (BPPhy), cysteine phytases (CPhy), and histidine acid phytases (HAPhy) were found in bacteria ( Singh et al, 2020 ), among which BPPhy was the most widely distributed and was considered a good biomarker for evaluating soil organic P transformation ( Kumar et al, 2017 ; Lin et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

In-depth characterization of phytase-producing plant growth promotion bacteria isolated in alpine grassland of Qinghai-Tibetan Plateau

Yang

et al. 2023

Front. Microbiol.

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The use of plant growth promoting bacteria (PGPB) express phytase (myo-inositol hexakisphosphate phosphohydrolase) capable of hydrolyzing inositol phosphate in soil was a sustainable approach to supply available phosphorus (P) to plants. A total of 73 bacterial isolates with extracellular phytase activity were selected from seven dominant grass species rhizosphere in alpine grassland of Qinghai-Tibetan Plateau. Then, the plant growth promoting (PGP) traits of candidate bacteria were screened by qualitative and quantitative methods, including organic/inorganic Phosphorus solubilization (P. solubilization), plant hormones (PHs) production, nitrogen fixation, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and antimicrobial activity. Further experiment were conducted to test their growth promoting effect on Lolium perenne L. under P-limitation. Our results indicated that these bacteria as members of phyla Proteobacteria (90.41%) and Actinobacteria (9.59%) were related to 16 different genera. The isolates of Pseudomonas species showed the highest isolates number (36) and average values of phytase activity (0.267 ± 0.012 U mL–1), and showed a multiple of PGP traits, which was a great candidate for PGPBs. In addition, six strains were positive in phytase gene (β-propeller phytase, bpp) amplification, which significantly increased the shoot length, shoot/root fresh weight, root average diameter and root system phytase activity of Lolium perenne L. under P-limitation, and the expression of phytase gene (bppP) in root system were verified by qPCR. Finally, the PHY101 gene encoding phytase from Pseudomonas mandelii GS10-1 was cloned, sequenced, and recombinantly expressed in Escherichia coli. Biochemical characterization demonstrated that the recombinant phytase PHY101 revealed the highest activity at pH 6 and 40°C temperature. In particular, more than 60% of activity was retained at a low temperature of 15°C. This study demonstrates the opportunity for commercialization of the phytase-producing PGPB to developing localized microbial inoculants and engineering rhizobacteria for sustainable use in alpine grasslands.

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“…The peaks identified in Fig 1B for kidney tissue samples are also the isomers present in liver tissue samples ( Fig 1A ). The identities of peaks in the set of standards ( Fig 1C ) have been described [ 20 , 36 , 39 ]. The isomers detected in tissues, D-and/or L-Ins(3,4,5,6)P 4 and Ins(1,3,4,5,6)P 5 , differ from the known products of phytate degradation in Quantum Blue-supplemented diets, D-and/or L-Ins(1,2,3,4)P 4 , D-and/or L-Ins(1,2,5,6)P 4 , Ins(1,2,3,4,6)P 5 , D-and/or L-Ins(1,2,3,4,5)P 5 and D-and/or L-Ins(1,2,4,5,6)P 5 [ 40 ] ( Fig 1D and 1E ).…”

Section: Resultsmentioning

confidence: 99%

Phytase dose-dependent response of kidney inositol phosphate levels in poultry

Sprigg

Whitfield²,

Burton³

et al. 2022

PLoS ONE

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Phytases, enzymes that degrade phytate present in feedstuffs, are widely added to the diets of monogastric animals. Many studies have correlated phytase addition with improved animal productivity and a subset of these have sought to correlate animal performance with phytase-mediated generation of inositol phosphates in different parts of the gastro-intestinal tract or with release of inositol or of phosphate, the absorbable products of phytate degradation. Remarkably, the effect of dietary phytase on tissue inositol phosphates has not been studied. The objective of this study was to determine effect of phytase supplementation on liver and kidney myo-inositol and myo-inositol phosphates in broiler chickens. For this, methods were developed to measure inositol phosphates in chicken tissues. The study comprised wheat/soy-based diets containing one of three levels of phytase (0, 500 and 6,000 FTU/kg of modified E. coli 6-phytase). Diets were provided to broilers for 21 D and on day 21 digesta were collected from the gizzard and ileum. Liver and kidney tissue were harvested. Myo-inositol and inositol phosphates were measured in diet, digesta, liver and kidney. Gizzard and ileal content inositol was increased progressively, and total inositol phosphates reduced progressively, by phytase supplementation. The predominant higher inositol phosphates detected in tissues, D-and/or L-Ins(3,4,5,6)P4 and Ins(1,3,4,5,6)P5, differed from those (D-and/or L-Ins(1,2,3,4)P4, D-and/or L-Ins(1,2,5,6)P4, Ins(1,2,3,4,6)P5, D-and/or L-Ins(1,2,3,4,5)P5 and D-and/or L-Ins(1,2,4,5,6)P5) generated from phytate (InsP6) degradation by E. coli 6-phytase or endogenous feed phytase, suggesting tissue inositol phosphates are not the result of direct absorption. Kidney inositol phosphates were reduced progressively by phytase supplementation. These data suggest that tissue inositol phosphate concentrations can be influenced by dietary phytase inclusion rate and that such effects are tissue specific, though the consequences for physiology of such changes have yet to be elucidated.

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Improved sensitivity, accuracy and prediction provided by a high‐performance liquid chromatography screen for the isolation of phytase‐harbouring organisms from environmental samples

Cited by 6 publications

References 66 publications

Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation

Strategies of organic phosphorus recycling by soil bacteria: acquisition, metabolism, and regulation

In-depth characterization of phytase-producing plant growth promotion bacteria isolated in alpine grassland of Qinghai-Tibetan Plateau

Phytase dose-dependent response of kidney inositol phosphate levels in poultry

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