Improved somatic embryogenesis from Cocos nucifera (L.) plumule explants

Pérez-Núñez, M. T.; Chan, José Luis; Sáenz, Luis; González, Tania; Verdeil, Jean‐Luc; Oropeza, Carlos

doi:10.1079/ivp2005722

Cited by 81 publications

(86 citation statements)

References 19 publications

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“…However, it is worth noting that during the past three decades many efforts have been undertaken to optimise the procedure for rapid multiplication of the normal coconut genotype via SE (Reviewed in [2]). Successful and consistent plantlet production of normal coconut through SE has been achieved using plumule [81], unfertilized ovary [82], and immature inflorescences [83] as the explant. Among these potential explants, the plumule is ideal for multiplication of makapuno M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT through SE.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

“…However, somatic tissues can be successfully utilised only if the donor makapuno-bearing plants are raised using embryo culture technology. Certain measures such as the introduction of a callus multiplication phase [81] and prior selection of amenable palms for SE have been identified for maximising the plant production through SE. Such inventions can be taken into consideration when developing a protocol for micropropagation of the makapuno coconut.…”

Section: Somatic Embryogenesismentioning

confidence: 99%

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Biology, propagation and utilization of elite coconut varieties (makapuno and aromatics)

Nguyen

Bandupriya

Foale

et al. 2016

Plant Physiology and Biochemistry

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Coconut farming is not only a vital agricultural industry for all tropical countries possessing humid coasts and lowlands, but is also a robust income provider for millions of smallholder farmers worldwide. However, due to its longevity, the security of production of this crop suffers significantly from episodes of natural disasters, including cyclone and tsunami, devastating pest and disease outbreaks, while also affected by price competition for the principal products, especially the oil. In order to reduce these pressures, high-value coconut varieties (makapuno and aromatics) have been introduced in some regions, on a limited scale, but with positive outcomes. Even though these two varieties produce fruit with delicious solid or flavoursome liquid endosperm, their distinct biochemical and cellular features unfortunately prevent their in situ germination. In fact, embryo rescue and culture have been developed historically to nurture the embryo under in vitro conditions, enabling effective propagation. In an attempt to provide a comprehensive review featuring these elite coconut varieties, this paper firstly introduces their food values and nutritional qualities, and then discusses the present knowledge of their biology and genetics. Further possibilities for coconut in general are also highlighted, through the use of advanced tissue culture techniques and efficient seedling management for sustainable production of these highly distinct and commercially attractive varieties of coconut.

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Section: Somatic Embryogenesismentioning

confidence: 99%

Section: Somatic Embryogenesismentioning

confidence: 99%

Biology, propagation and utilization of elite coconut varieties (makapuno and aromatics)

Nguyen

Bandupriya

Foale

et al. 2016

Plant Physiology and Biochemistry

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“…Bombardment of embryogenic calli was carried out using 1 µm diameter gold particles (without DNA) at a distance of 6 cm with a pressure of 900 psi. The coconut embryogenic calli were placed in the middle of a petri dish forming a circle of 1.5 cm in diameter over the semisolid Y3 medium phase I (Pérez-Núñez et al 2006). After biobalistic treatment, the embryogenic calli were submerged in YEP medium or bacterial suspension with A. tumefaciens strain C58C1 + pER10W-35SRed (Table 1).…”

Section: Transient Transformation By Biobalistics Vacuum Infiltratiomentioning

confidence: 99%

“…Coconut is recalcitrant to in vitro morphogenesis . Nevertheless, we recently reported a reproducible regeneration system for plumule explants based on the multiplication of embryogenic callus and secondary somatic embryogenesis, with which several thousand somatic embryos can be obtained per initial explant (Pérez-Núñez et al 2006). However, efficiency is still low in terms of the number of explants producing embryogenic callus and the number of somatic embryos generated per embryogenic callus.…”

Section: Introductionmentioning

confidence: 99%

“…Wild type alfalfa seedlings germinated in the MS medium were used as reference for negative GUS activity. In subsequent experiments (using fluorescent proteins as reporter genes, experiments for Agrobaterium elimination and experiments for kanamycin as selective agent), we used embryogenic calli obtained from plumular explants, according to Pérez-Núñez et al (2006).…”

Section: Introductionmentioning

confidence: 99%

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Transient genetic transformation of embryogenic callus of Cocos nucifera

et al. 2011

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Coconut palm (Cocos nucifera) is a plant species recalcitrant to in vitro morphogenesis and no protocols for the genetic transformation of coconut tissues have been published. The present study aimed to develop a protocol for genetic transformation of this palm species; evaluating reporter genes, transformation methods, and conditions for the use of antibiotics to select transformed plant cells. The gene gusA was first used for Agrobacterium tumefaciens mediated transformation of coconut embryogenic calli. However, endogenous GUS-like activity was found in calli not co-cultured with bacteria. Then essays for Agrobacterium-mediated transformation were developed using green and red fluorescent genes. Both genes are suitable as reporter genes for coconut transformation. In order to establish a protocol for coconut genetic transformation, an approach was used that combined biobalistics to generate micro-wounds in explants, vacuum infiltration and co-culture with Agrobacterium tumefaciens (C58C1 + pER10W-35SRed containing the embryogenesis related gene WUSCHEL). Calli treated with the combined protocol showed red fluorescence with greater intensity and greater area than calli treated with either biobalistics or infiltration, followed by bacteria co-culture. PCR amplification of DNA extracts from transformed embryogenic callus produced a band with the expected size using WUSCHEL primers (862 bp). No band was obtained using the VirE2 primers. This is the first report of transient genetic transformation of C. nucifera and it is the first step toward a protocol that will be useful for the study of the role of genes of interest and for practical applications, such as the improvement of coconut micropropagation via somatic embryogenesis.

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