Improved specificity of reagentless amperometric PQQ-sGDH glucose biosensors by using indirectly heated electrodes

Lau, Carolin; Borgmann, Sabine; Maciejewska, Monika; Ngounou, Bertrand; Gründler, Peter; Schuhmann, Wolfgang

doi:10.1016/j.bios.2006.12.033

Cited by 43 publications

(22 citation statements)

References 35 publications

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“…Heated electrodes have been used in many applications including lead detection [16], formaldehyde, methanol and formic acid oxidation [17], anodic stripping voltammetry on heated mercury film electrode [18], electrochemical behavior of cytochrome C [19], identification of DNA damage [20], interaction between DNA and metal complexes [21], electrochemistry of nicotinamide adenine dinucleotide [22], improvement of glucose and maltose sensor specificity [23], electrochemistry of ascorbic acid [24], rutin detection in the nanomolar range [25], thermal stabilization of glucose heated electrodes [26], tool to prevent biochemical fouling on electrodes [27], capillary electrophoresis detectors [28], flow detectors [29], and disposable electrodes [30]. …”

Section: Introductionmentioning

confidence: 99%

Voltammetry under a Controlled Temperature Gradient

Sajdlová

Krejčí

Marvanek

2010

Sensors

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Electrochemical measurements are generally done under isothermal conditions. Here we report on the application of a controlled temperature gradient between the working electrode surface and the solution. Using electrochemical sensors prepared on ceramic materials with extremely high specific heat conductivity, the temperature gradient between the electrode and solution was applied here as a second driving force. This application of the Soret phenomenon increases the mass transfer in the Nernst layer and enables more accurate control of the electrode response enhancement by a combination of diffusion and thermal diffusion. We have thus studied the effect of Soret phenomenon by cyclic voltammetry measurements in ferro/ferricyanide. The time dependence of sensor response disappears when applying the Soret phenomenon, and the complicated shape of the cyclic voltammogram is replaced by a simple exponential curve. We have derived the Cotrell-Soret equation describing the steady-state response with an applied temperature difference.

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Section: Introductionmentioning

confidence: 99%

Voltammetry under a Controlled Temperature Gradient

Sajdlová

Krejčí

Marvanek

2010

Sensors

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“…The squeezing of the glucose response with increasing AA concentration can be seen and it leads to a deviation of calibration curves from linearity already at rather low glucose concentrations. Based on the obtained calibration plots for glucose and AA it would be straight forward to derive glucose concentrations for known and constant AA concentrations using the reversed Michaelis equation [26]. Equally, AA calibration graphs could be fitted with 2 nd order polynomials for determination of the AA concentration.…”

Section: Selection Of Optimal Sensing Sites For Determination Of Glucmentioning

confidence: 99%

“…The overall signal is then dependent on the concentrations of both substrates, their affinity to the enzymes active site (K M values), their individual reaction rates as well as their diffusional mass transport properties. Recently, a first step into this problem was done by evaluation the coselectivity of PQQ-dependent glucose dehydrogenase for glucose and maltose using heated electrodes [26]. 2-deoxy glucose was identified to act as competitive cosubstrate for glucose oxidase [27].…”

Section: Introductionmentioning

confidence: 99%

SECM Visualization of Spatial Variability of Enzyme‐Polymer Spots. Part 2: Complex Interference Elimination by Means of Selection of Highest Sensitivity Sensor Substructures and Artificial Neural Networks

2006

Self Cite

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Polymer spots with entrapped glucose oxidase were fabricated on glass surfaces and the localized enzymatic response was subsequently visualized using scanning electrochemical microscopy (SECM) in the generator -collector mode. SECM images were obtained under simultaneous variation of the concentration of glucose (0 -6 mM) and ascorbic acid (0 -200 mM), or, in a second set of experiments, of glucose (0 -2 mM) and 2-deoxy-d(þ)-glucose (0 -4 mM). Aiming at the quantification of the mixture components discretization of the response surfaces of the overall enzyme/ polymer spot into numerous spatially defined microsensor substructures was performed. Sensitivity of sensor substructures to measured analytes was calculated and patterns of variability in the data were analyzed before and after elimination of interferences using principal component analysis. Using artificial neural networks which were fed with the data provided by the sensor substructures showing highest sensitivity for glucose, glucose concentration could be calculated in solutions containing unknown amounts of ascorbic acid with a good accuracy (RMSE 0.17 mM). Using, as an input data set, measurements provided by sensing substructures showing highest sensitivity for ascorbic acid in combination with the response of the sensors showing highest dependence on the glucose concentration, the error of the ascorbic acid concentration calculation in solution containing the unknown amount of glucose was 10 mM. Similarly, prediction of the glucose concentration in the presence of 2-deoxy-d(þ)-glucose was possible with a RMSE of 0.1 mM while the error of the calculation of 2-deoxy-d(þ)-glucose concentrations in the presence of unknown concentrations of glucose was 0.36 mM.

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“…Mediators that have been adopted in the implementation of PQQ-GDH biosensors with useful response signals include osmium complexes [4–6], ruthenium complexes [7,8], N -methyl-phenazonium [9], ferrocene derivatives [10], cytochrome b562 [11], ferricyanide [12], carbon nanotubes [13] and gold nanoparticles [14]. Polymers such as polypyrrole [15–17], polyurethane [18] and poly(acrylate) [19] have been used for the immobilization of PQQ-GDH enzymes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Electric Potential Treatment of a Chromium Hexacyanoferrate Modified Biosensor Based on PQQ-Dependent Glucose Dehydrogenase

Tseng

Yang

Lin

et al. 2010

Sensors

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A novel potential treatment technique applied to a glucose biosensor that is based on pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) and chromium hexacyanoferrate (CrHCF) incorporated into a platinum (Pt) electrode was demonstrated. CrHCF, serving as a mediator, was electrochemically deposited on the Pt electrode as ascertained by CV, SEM, FTIR and XPS measurements. The potential treatment of CrHCF, which converts Fe(II) to Fe(III), enables the glucose detection. The amperometric measurement linearity of the biosensor was up to 20 mM (R = 0.9923), and the detection sensitivity was 199.94 nA/mM per cm2. More importantly, this biosensor remained stable for >270 days.

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Improved specificity of reagentless amperometric PQQ-sGDH glucose biosensors by using indirectly heated electrodes

Cited by 43 publications

References 35 publications

Voltammetry under a Controlled Temperature Gradient

Voltammetry under a Controlled Temperature Gradient

SECM Visualization of Spatial Variability of Enzyme‐Polymer Spots. Part 2: Complex Interference Elimination by Means of Selection of Highest Sensitivity Sensor Substructures and Artificial Neural Networks

Effects of Electric Potential Treatment of a Chromium Hexacyanoferrate Modified Biosensor Based on PQQ-Dependent Glucose Dehydrogenase

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