Improved theory of cyclical electrical field flow fractionation

Kantak, Ameya; Merugu, Srinivas; Gale, Bruce K.

doi:10.1002/elps.200500831

Cited by 14 publications

(30 citation statements)

References 12 publications

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“…A more complete understanding of the inner workings of an ElFFF channel has not yet been presented, though empirical models have been presented by Kantak et al 10,13 .…”

Section: Theorymentioning

confidence: 98%

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Nanoparticle analysis using microscale field flow fractionation

Gale

Sant

2007

Microfluidics, BioMEMS, and Medical Microsystems V

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Microscale electrical field flow fractionation (FFF) has shown significant progress since it was first reported in early 1997. The first electrical FFF systems were lucky to function for more than a few days and generated only minimal levels of retention and separation, while current systems now easily function for years and can generate multicomponent nanoparticle separations. A variety of microscale FFF systems have now been reported including: multiple versions of normal electrical FFF (ElFFF), cyclical electrical FFF (using oscillating fields), dielectrophoretic FFF, thermal FFF, and a combined thermal-electric FFF channel. Related microscale electrical SPLITT systems have also been demonstrated. Microscale ElFFF systems have been used to analyze and separate nanoparticles, DNA, proteins, cells, viruses, liposomes, large polymers, and other materials. ElFFF clearly improves upon system miniaturization due to the reduction in sample and carrier volumes, analysis times and more notably an increase in the separation resolution with a reduction in analysis times. Other advantages of miniaturized FFF include: parallel processing with multiple separation channels, batch fabrication with reduced costs, high quality manufacturing, and potentially disposable systems. Additionally, the possibility of on-chip sample injection, detection and signal processing favors the microfabrication of FFF systems.

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“…A more complete understanding of the inner workings of an ElFFF channel has not yet been presented, though empirical models have been presented by Kantak et al 10,13 .…”

Section: Theorymentioning

confidence: 98%

“…shows the typical elution characteristics of the µ-CyElFFF as a function of frequency of the applied field13 . It can be deduced fromFig.…”

mentioning

confidence: 99%

Nanoparticle analysis using microscale field flow fractionation

Gale

Sant

2007

Microfluidics, BioMEMS, and Medical Microsystems V

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“…In the published experiment (Kantak et al 2006), 100 nm charged polystyrene (density 1.05 g/cm 3 ) nanoparticles was used for separation in a microchannel flow (channel length 5.1 cm and thickness 30 lm) at a flow rate of 10 ml/min. 3 volts of (peak to peak) square wave electric field was applied at frequencies of 20, 30 and 70 Hz and the elution time were recorded.…”

Section: Validation With Experiments and Theorymentioning

confidence: 99%

“…6. The simulation results on elution time are also plotted with experimental results (Kantak 2005) in Fig. 5.…”

Section: Validation With Experiments and Theorymentioning

confidence: 99%

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Modeling of micro/nano particle separation in microchannels with field-flow fractionation

et al. 2010

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The cyclical electrical field-flow fractionation (CyElFFF) is a very promising separation technique for particles and biological molecules such as proteins, nucleic acids, viruses, bacteria, yeast cells, mammalian cells. But a clear understanding of the mechanism and performance prediction of this system under different operating parameters is far from completed. This research focuses on a computational investigation of particle behavior in a CyElFFF system by taking into account both electrokinetic effects and particle dynamics. The model was validated with both theory and experimental results. The effects of key parameters such as applied electric field strength and frequency, solution fluid flow rate, particle size, particle shape on separation process are addressed in a systematic way. The developed model can also be utilized in studying the behavior of spherical or non-spherical particles (such as nanowire, nanorod, and nanofiber) in other microfluidic systems.

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Optimization of cyclical electrical field flow fractionation

2010

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Cyclical electrical field flow fractionation (CyElFFF) is a variation on electrical field flow fractionation (ElFFF) where cyclical electrical fields are used instead of steady DC fields to increase the effective field experienced by particles in the flow channel. Even though the effective field increases more than 20-fold compared to normal ElFFF, the retention and resolution in CyElFFF has not been shown to be better than in ElFFF. In this paper we report how one can optimize operational parameters in CyElFFF to obtain good retention and resolution in CyElFFF. The effects of offset voltage, frequency, flowrate, concentration of particles and sample size on retention, resolution and retained peak/void peak ratio have been observed. The results obtained from these experiments were analyzed and suggestions have been made to improve both retention and resolution. A 4-fold improvement in retention without a significant increase in band broadening is reported.

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Improved theory of cyclical electrical field flow fractionation

Cited by 14 publications

References 12 publications

Nanoparticle analysis using microscale field flow fractionation

Nanoparticle analysis using microscale field flow fractionation

Modeling of micro/nano particle separation in microchannels with field-flow fractionation

Optimization of cyclical electrical field flow fractionation

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