Improvement in endothelial cell adhesion and retention under physiological shear stress using a laminin–apatite composite layer on titanium

He, Fupo; Wang, Xiupeng; Maruyama, Osamu; Kosaka, Ryo; Sogo, Yu; Ito, Atsuo; Ye, Jiandong

doi:10.1098/rsif.2013.0014

Cited by 7 publications

(3 citation statements)

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“…These proteins have highly conserved functions and can serve as anchors for endothelial cells under shear stress conditions (70). Laminin α4 is permissive for transmigration of neutrophils, T cells, and even parasites through blood vessels, whereas laminin α5 is inhibitory (71)(72)(73).…”

Section: Days) Data Presented As Mean ± Sem (A-d) or Percent Survivamentioning

confidence: 99%

Laminins affect T cell trafficking and allograft fate

Warren¹,

Iwami²,

Harris³

et al. 2014

J. Clin. Invest.

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Lymph nodes (LNs) are integral sites for the generation of immune tolerance, migration of CD4 + T cells, and induction of Tregs. Despite the importance of LNs in regulation of inflammatory responses, the LN-specific factors that regulate T cell migration and the precise LN structural domains in which differentiation occurs remain undefined. Using intravital and fluorescent microscopy, we found that alloreactive T cells traffic distinctly into the tolerant LN and colocalize in exclusive regions with alloantigen-presenting cells, a process required for Treg induction. Extracellular matrix proteins, including those of the laminin family, formed regions within the LN that were permissive for colocalization of alloantigen-presenting cells, alloreactive T cells, and Tregs. We identified unique expression patterns of laminin proteins in high endothelial venule basement membranes and the cortical ridge that correlated with alloantigen-specific immunity or immune tolerance. The ratio of laminin α4 to laminin α5 was greater in domains within tolerant LNs, compared with immune LNs, and blocking laminin α4 function or inducing laminin α5 overexpression disrupted T cell and DC localization and transmigration through tolerant LNs. Furthermore, reducing α4 laminin circumvented tolerance induction and induced cardiac allograft inflammation and rejection in murine models. This work identifies laminins as potential targets for immune modulation.

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Section: Days) Data Presented As Mean ± Sem (A-d) or Percent Survivamentioning

confidence: 99%

Laminins affect T cell trafficking and allograft fate

Warren¹,

Iwami²,

Harris³

et al. 2014

J. Clin. Invest.

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“…A signicant increase (*P < 0.05) of HUVECs proliferation on G@A@5Ln was observed aer incubation of 1 day ( Fig. That could be explained by that laminin shows specic binding sites to cells, 18,46,47 thereby facilitating HUVECs and EPCs adhesion, 17,48,49 which make laminin essential for devices applied in vascular applications. In the same way, the amounts of EPCs on G@A@5Ln were signicantly more (*P < 0.05) than that on glass, G@APTE surface and the other Ln/F samples, aer incubation for 1 day (Fig.…”

Section: Cytocompatibility Of Huvecs and Epcsmentioning

confidence: 93%

“…There is more EPCs adhesion and fully spreading on the samples for an outermost layer of laminin, compared with that for the outermost layer of fucoidan. That could be explained by that laminin shows specic binding sites to cells, 18,46,47 thereby facilitating HUVECs and EPCs adhesion, 17,48,49 which make laminin essential for devices applied in vascular applications.…”

Section: (C) and (D))mentioning

confidence: 99%

Layer-by-layer self-assembled laminin/fucoidan films: towards better hemocompatibility and endothelialization

Wang

et al. 2016

RSC Adv.

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Rapid endothelialization is an effective solution to thrombosis and restenosis in vascular-implanting materials. Surface modification is a concise and impactful approach to improve biocompatibility including endothelialization and hemocompatibility. In this paper, we report on a layer-by-layer selfassembly of laminin/fucoidan multilayers for the improvement of hemocompatibility and endothelialization. The chemical changes and wettability of the surface assembled films were investigated by static water contact angle measurement, X-ray photoelectron spectroscopy, colorimetric and immunosorbent assay. The real time assembly process was in situ monitored by quartz crystal microbalance with dissipation. The platelets adhesion/activation test showed that significantly fewer platelets were adherent and activated on the surface of assembled three layer laminin/fucoidan samples with the outermost layer of fucoidan. Furthermore, cell culture tests indicated that the adhesion of human umbilical vein endothelial cells and endothelial progenitor cells were promoted on the surface of an assembled five layer laminin/fucoidan sample with the outermost layer of laminin. It is concluded that layer-by-layer self-assembled laminin/fucoidan multilayers are a promising candidate for a biofunctional coating applied to vascular implants. Fig. 9 Cellular compatibility evaluation results; (a) and (c): immunofluorescence staining of ECs and EPCs after 1 day culture; (b) and (d): related cell counting results of each sample (mean AE SD, n ¼ 3, *P < 0.5). 56054 | RSC Adv., 2016, 6, 56048-56055 This journal is

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