Improvement of bioactive compound accumulation in cell cultures of Orostachys cartilaginous A. Bor. through elicitation with salicylic acid and effect of cell extract on bioactive activity

Tian, Wen; Hao, Yanling; An, Xiaolong; Sun, Hao-Ding; Li, Yanru; Chen, Xue; Piao, Xiangfan; Lian, Mei-Lan

doi:10.1016/j.indcrop.2019.111570

Cited by 28 publications

(15 citation statements)

References 29 publications

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“…It may be connected to the increased level of salicylic acid in these clones. Our results agree with data presented by Wen et al ( 2019 ), where P. aeruginosa was more resistant to Orostachys cartilaginous -derived extracts in comparison to the Gram-positive pathogen Bacillus subtilis. Except for plumbagin, which was proven to be biologically active and potent, phenolic acids have a crucial role in the antibacterial activity of medicinal plants.…”

Section: Discussionsupporting

confidence: 93%

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Makowski

Królicka

Nowicka

et al. 2021

Appl Microbiol Biotechnol

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The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.

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Section: Discussionsupporting

confidence: 93%

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Makowski

Królicka

Nowicka

et al. 2021

Appl Microbiol Biotechnol

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“…Callus cultures of Zingiber officinale treated with 50 mg/L SA significantly induced a high amount of TPC [ 11 ]. The cell culture of Orostachys cartilaginous treated with 100 μM SA also showed an optimum production of TPC and TFC [ 19 ]. In agreement with our results, Ocimum bacilicum cell suspension cultures treated with 200 mg/L YE showed the highest TPC and TFC [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Contrary to our findings, Ocimum bacilicum cell cultures treated with YE accumulated the highest chicoric acid and rosmarinic acid when compared to the control [ 14 ]. The concentrations of SA from 25 to 150 μM enhanced the bioactive compound accumulation in cell culture of Orostachys cartilaginous without any reduction in the cell biomass [ 19 ]. In cell suspension culture of Salvia miltiorrhiza, SA as an elicitor significantly increased the biosynthesis of 10 bioactive compounds at different times [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Elicitor-Induced Production of Biomass and Pharmaceutical Phenolic Compounds in Cell Suspension Culture of Date Palm (Phoenix dactylifera L.)

Al-Khayri

Naik

2020

Molecules

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Plants that synthesize bioactive compounds that have high antioxidant value and elicitation offer a reliable in vitro technique to produce important nutraceutical compounds. The objective of this study is to promote the biosynthesis of these phenolic compounds on a large scale using elicitors in date palm cell suspension culture. Elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2), and silver nitrate (AgNO3) at 50, 100, and 200 mg/L concentrations are used. The effects of elicitors on cell culture were determined in terms of biomass [packed cell volume (PCV), fresh and dry weight], antioxidant activity, and phenolic compounds (catechin, caffeic acid, kaempferol, apigenin) were determined using high-performance liquid chromatography (HPLC). Results revealed that enhanced PCV (12.3%), total phenolic content [317.9 ± 28.7 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)], and radical scavenging activity (86.0 ± 4.5%) were obtained in the 50 mg/L SA treated cell culture of Murashige and Skoog (MS) medium. The accumulation of optimum catechin (26.6 ± 1.3 µg/g DW), caffeic acid (31.4 ± 3.8 µg/g DW), and kaempferol (13.6 ± 1.6 µg/g DW) was found in the 50 mg/L SA-treated culture when compared to the control. These outcomes could be of great importance in the nutraceutical and agronomic industries.

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“…Stress factors strongly influence the production of secondary metabolites in plants. The use of elicitors such as methyl jasmonate, salicylic acid, and glutathione, chitosan is a known way to increase the production of flavonoid compounds in plant cultures in vitro [59][60][61][62]. Ultraviolet radiation has a similar effect on the accumulation of flavonoid compounds in plants [63][64][65][66][67].…”

Section: Resultsmentioning

confidence: 99%

The Callus of Phaseolus coccineus and Glycine max Biotransform Flavanones into the Corresponding Flavones

2020

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In vitro plant cultures are gaining in industrial importance, especially as biocatalysts and as sources of secondary metabolites used in pharmacy. The idea that guided us in our research was to evaluate the biocatalytic potential of newly obtained callus tissue towards flavonoid compounds. In this publication, we describe new ways of using callus cultures in the biotransformations. In the first method, the callus cultures grown on a solid medium are transferred to the water, the reaction medium into which the substrate is introduced. In the second method, biotransformation is carried out on a solid medium by growing callus cultures. In the course of the research, we have shown that the callus obtained from Phaseolus coccineus and Glycine max is capable of converting flavanone, 5-methoxyflavanone and 6-methoxyflavanone into the corresponding flavones.

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Improvement of bioactive compound accumulation in cell cultures of Orostachys cartilaginous A. Bor. through elicitation with salicylic acid and effect of cell extract on bioactive activity

Cited by 28 publications

References 29 publications

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Elicitor-Induced Production of Biomass and Pharmaceutical Phenolic Compounds in Cell Suspension Culture of Date Palm (Phoenix dactylifera L.)

The Callus of Phaseolus coccineus and Glycine max Biotransform Flavanones into the Corresponding Flavones

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