Improvement of carbon source composition reduces antibiotic resistance genes in the ectopic fermentation system

Luo, Guoan; Liu, Min; Zeng, Jinjie; Huang, Shuntao; Huang, Jingshu; Ahmed, Zubair; Yang, Yaokun; Lai, Renhao; Xu, Dequan

doi:10.1016/j.biortech.2023.129064

Cited by 5 publications

(1 citation statement)

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“…The ADFom (acid detergent fiber expressed exclusive of residual ash) and aNDFom (neutral detergent fiber with heat-stable amylase and expressed exclusive of residual ash) were analyzed following the methods of Van Soest et al ( 23 ). Cellulose was calculated as ADFom minus lignin ( 24 ). Non-fiber carbohydrates were calculated using the following formula: 100 − % aNDFom − % CP − % EE − % ash ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

Treatment of corn with lactic acid delayed in vitro ruminal degradation without compromising fermentation: a biological and morphological monitoring study

Tian,

Luo,

Aldian

et al. 2024

Front. Vet. Sci.

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Grain processed by lactic acid (LA) is known to improve ruminant growth and health. However, the exact mechanism regarding rumen hydrolysis of LA-treated grain is still ambiguous. This experiment was designed to compare the effects of 5% LA treatment on the trophic and morphological variations in corn and to discover the alternations in ruminal hydrolysis between LA-treated and untreated corn macroscopically and microscopically using in vitro fermentation method. The results showed that, compared with untreated corn (CN), corn treated with 5% LA for 48 h (CNLA) experienced a decrease in the dry matter, albumin fraction, aNDFom, and water-soluble carbohydrate content but an increase in the resistant starch content. The in vitro fermentation showed that the pH of CNLA was higher, but dry matter disappearance was lower than that of CN. Most of the fermentation indices were unaffected, except for decreased iso-butyrate and iso-valerate. The abundances of total bacteria, Prevotella spp., Streptococcus bovis, and Selenomonas ruminantium were higher, but those of Ruminococcus flavefaciens and Ruminococcus albus were lower in CNLA than in CN. There were differences in the scanning electron micrographs between CNLA and CN after 3 h of fermentation. This study suggests that treating corn with LA for 48 h can induce changes in its nutrient composition and alter the bacterial flora during subsequent in vitro fermentation. These changes appeared to be crucial contributors to the beneficial effects observed in rumen fermentation.

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Section: Methodsmentioning

confidence: 99%