Improvement of exopolysaccharide production by macro-fungus Auricularia auricula in submerged culture

Efficient Production of Exopolysaccharide by Submerged Fermentation of Hypsizygus marmoreus Using a Two-stage pH Control Strategy

“…was similar to that previously reported by Wu et al 15 , who indicated that a lower initial pH (pH 5.0) was beneficial to biomass accumulation, and that a higher initial pH (pH 5.5) contributed to EPS production by A. auricula.…”

“…However, this is time-consuming, low-productive and economically unviable, and the production rate is insufficient to meet the current market demand. Submerged culture has been a fast alternative method to produce medicinal mushroom mycelia and bioactive compounds, and a large amount of studies have been focusing on the polysaccharide production by growing mushroom mycelium in liquid culture with defined nutrients [6][7][8]14,15 . A vast range of valuable polysaccharide has been extensively exploited from medicinal mushrooms by submerged culture, such as G. lucidum, Grifola frondosa, C. militaris [6][7][8][9]16 .…”

“…Mycelial growth and metabolism can be significant-* ly affected by media composition and other chemical and physical factors in submerged culture, and pH is one of the vital parameters because it can influence the solubility of salts, the ionic state of substrates, cell membrane function, and metabolite biosynthesis 19 . Various pH conditions were studied and two-stage pH strategy had been utilized to enhance the mycelial growth and product biosynthesis in some fungi fermentation 14,15,[19][20][21] . The polysaccharide productivity of Auricularia auricula increased by 93.3 % when culture pH was controlled at 5.0 in the first 48 h and then switched to 5.5 compared with that of non-pH control 15 .…”

“…Various pH conditions were studied and two-stage pH strategy had been utilized to enhance the mycelial growth and product biosynthesis in some fungi fermentation 14,15,[19][20][21] . The polysaccharide productivity of Auricularia auricula increased by 93.3 % when culture pH was controlled at 5.0 in the first 48 h and then switched to 5.5 compared with that of non-pH control 15 . The maximum concentration of rhamsan gum was 12.83 % higher when the culture pH of Sphingomonas sp.…”

Hypocholesterolemic effects of Auricularia auricula ethanol extract in ICR mice fed a cholesterol-enriched diet

“…Previous studies have also shown that A. auricula polysaccharides have similar biological activities as A. auricula. Many nutritionists probed into A. auricula polysaccharide components (Aletor 1995), conformational change of A. auricula polysaccharides (Zhang et al 1995a), molecular weights of A. auricula polysaccharides (Zhang et al 1995b), improvement of production of A. auricula polysaccharides (Wu et al 2006) and impact on lipid metabolism . Most recently, some health-promoting diet formulae have been developed using A. auricula as the major ingredient with combination of other nutritional herbal foods (Luo et al 2009a, b).…”