Improvements in the Separation Capabilities of Sequential Injection Chromatography: Determination of Intracellular Dissolved Free Amino Acid Profiles in Three Taxonomic Groups of Microalgae

Abstract

Resolutions of the amino acid pairs glutamine and histidine, valine and phenylalanine, and isoleucine and leucine were 1.5, 0.75 and 1.3, respectively. The proposed method found different profiles of DFAA among the three species of algae, suggesting its adequacy for metabolic studies.

Sequential Injection Chromatography with an Ultra-short Monolithic Column for the Low-Pressure Separation of α-Tocopherol and γ-Oryzanol in Vegetable Oils and Nutrition Supplements

“…For example, SIC multi-stepwise separation of as many as 19 out of 20 amino acids has been reported. 32 A short column with a short separation distance is also more sensitive to a change of the mobile phase polarity. However, both the flow rate and the mobile phase composition are controllable.…”

Evaluation of sequential injection chromatography for reversed phase separation of triazine herbicides exploiting monolithic and core–shell columns

“…The sequential injection systems used in the early SIC applications were prone to leaking of mobile phase, but mechanical reconfigurations with high-and medium-pressure components of high performance liquid chromatographs enabled the construction of equipments suitable to handle pressures up to 1000 psi [3,4]. This medium-pressure configuration enhanced the separation capabilities of SIC, enabling the use of modern short columns packed with core-shell particles, as well as narrow-bore monolithic columns [5].…”

Optimizing SIC assay method for acetyl salicylic acid and rosuvastatin and adapting to HPLC with performance comparison

“…For optimizing flow rate in SIC, the suitable range has been found to be 20-40 μL s −1 [10][11][12][13][14][15][16][17][18][19][20][21]. It is well-known that high flow rate accelerates analysis and sharpens peaks.…”

“…This problem limits the use of long separation column and hence limits the separation of many analytes despite nineteen chromatographic peaks for amino acids were observed in a previous study [16]. Another limitation of SIC is that the limited volume of the syringe.…”

“…SIC has proved to be an effective alternative for separation and quantification procedures with a special concern to pharmaceutical [10][11][12][13][14][15], phytochemical [16,17], biochemical [18,19], environmental [13,20], and food analysis [13,21]. The joint use of SIA, as a simple miniaturized handling technique, and a monolithic column, has generated SIC that is less expensive than HPLC.…”