Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system

Fuente, De La; Neil, Gerald

doi:10.31274/etd-180810-4353

Cited by 4 publications

(13 citation statements)

References 61 publications

(93 reference statements)

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“…The use of alternative chemicals with lower cytotoxicity has also been reported (reviewed in [6]). At the same time, haploid plants can undergo spontaneous haploid genome doubling (SHGD) with different efficiency rates depending on the crop and cultivar [127]. For example, in bread wheat, it was possible to achieve chromosome doubling at the rate of 70%, whereas, in barley, it was as high as 90% [128,129].…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Advances in Gene Editing of Haploid Tissues in Crops

Bhowmik¹,

Bilichak

2021

Genes

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Emerging threats of climate change require the rapid development of improved varieties with a higher tolerance to abiotic and biotic factors. Despite the success of traditional agricultural practices, novel techniques for precise manipulation of the crop’s genome are needed. Doubled haploid (DH) methods have been used for decades in major crops to fix desired alleles in elite backgrounds in a short time. DH plants are also widely used for mapping of the quantitative trait loci (QTLs), marker-assisted selection (MAS), genomic selection (GS), and hybrid production. Recent discoveries of genes responsible for haploid induction (HI) allowed engineering this trait through gene editing (GE) in non-inducer varieties of different crops. Direct editing of gametes or haploid embryos increases GE efficiency by generating null homozygous plants following chromosome doubling. Increased understanding of the underlying genetic mechanisms responsible for spontaneous chromosome doubling in haploid plants may allow transferring this trait to different elite varieties. Overall, further improvement in the efficiency of the DH technology combined with the optimized GE could accelerate breeding efforts of the major crops.

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Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Advances in Gene Editing of Haploid Tissues in Crops

Bhowmik¹,

Bilichak

2021

Genes

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“…The exploitation of SCD in maize on a commercial scale not only eliminates the need for colchicine treatment and transplantation, but also saves time, resources, and labor costs and reduces human health hazards and seed mutations linked with colchicine (De La Fuente, 2015). De La Fuente (2015) also reported that the cost of line development was reduced up to 91% when SCD was used rather than colchicine treatment.…”

Section: Chromosome Doubling Techniquesmentioning

confidence: 99%

“…De La Fuente, (2015) described another approach based on the VideometerLab3 spectral imaging system for haploid selection. Experiments (De La Fuente, 2015) showed that Videometer system holds significant potential for automated haploid selection based on the R1‐nj marker system in maize. Changes in the expression of the R1‐nj marker system usually make visual and automatic selection of haploids more difficult.…”

Section: Phenotypic Markersmentioning

confidence: 99%

“…Therefore seeds can be categorized into two different groups: easy to visually sort and difficult to visually sort. De La Fuente (2015) reported that seeds that are easy to visually sort were also accurately sorted by the VideometerLab3 system. In contrast, seeds which were difficult to visually sort needed to pass through the system several times to achieve appropriately accurate haploid sorting.…”

Section: Phenotypic Markersmentioning

confidence: 99%

“…In contrast, seeds which were difficult to visually sort needed to pass through the system several times to achieve appropriately accurate haploid sorting. Further validation with molecular markers showed that some of the haploids were correctly sorted through multiple runs in the VideometerLab3 system (De La Fuente, 2015).…”

Section: Phenotypic Markersmentioning

confidence: 99%

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Doubled haploids in maize: Development, deployment, and challenges

Maqbool¹,

Beshir²,

Khokhar³

2020

Crop Science

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Haploids are naturally produced in maize (Zea mays L.) at different rates and can also be induced through different methods. Haploids are used to develop doubled haploids (DHs), which have many potential uses. The development of DH lines in maize involves haploid induction, haploid identification, chromosome doubling, and field sowing for self‐pollination of D0 plants. Different potential methods are used for haploid induction, in‐vivo maternal haploid induction being the most prevalent. Haploid induction is highly reliant on the unambiguous identification of haploids among a mixture of different ploidies. Haploid identification is facilitated by visual morphological markers, chromosome counting, flow cytometry, molecular markers, and many other approaches. Chromosome doubling may be achieved by spontaneous doubling or by induction with different antimicrotubular treatments. Among the potential uses of DH lines are the development of inbred lines, genomic selection (GS), quantitative trait loci (QTL) mapping, and unlocking new genetic variations. Although DH technology can potentially accelerate maize breeding, it still faces challenges at each step of DH line development. This article aims to highlight the importance, procedural steps, potential opportunities, and key challenges in DH line development in maize.

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QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.)

et al. 2020

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Genome doubling of haploids is one of the major constraints of large-scale doubled haploid (DH) technology. Improving spontaneous haploid genome doubling (SHGD) is an alternative to overcome this limitation. In this study, we aimed to construct a high-density linkage map based on genotyping by sequencing (GBS) of Single Nucleotide Polymorphism (SNPs), to detect QTL and QTL by environment (Q by E) interactions affecting SHGD, and to identify the best trait for mapping and selection of haploid male fertility (HMF). To this end, a bi-parental population of 220 F2:3 families was developed from a cross between A427 (high HMF) and CR1Ht (moderate HMF) to be used as donor. A high-density linkage map was constructed containing 4,171 SNP markers distributed over 10 chromosomes with an average distance between adjacent markers of 0.51 cM. QTL mapping for haploid fertile anther emergence (AE), pollen production (PP), tassel size (TS), and HMF, identified 27 QTL across three environments, and Q by E interactions were significant. A major QTL was identified on chromosome 5. This QTL explained over 45% of the observed variance for all traits across all environments. The introgression of this major QTL, using marker-assisted backcrossing, has great potential to overcome the need of using colchicine in DH line development.

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Improvements to the maize (Zea mays L.) in vivo maternal doubled haploid system

Cited by 4 publications

References 61 publications

Advances in Gene Editing of Haploid Tissues in Crops

Advances in Gene Editing of Haploid Tissues in Crops

Doubled haploids in maize: Development, deployment, and challenges

QTL mapping of spontaneous haploid genome doubling using genotyping-by-sequencing in maize (Zea mays L.)

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