Improving 2-Phenylethanol Production via Ehrlich Pathway Using Genetic Engineered Saccharomyces cerevisiae Strains

Su, Yin; Zhou, Hui; Xiao, Xiao; Lang, Tiandan; Jingru, Liang; Wang, Chengtao

doi:10.1007/s00284-015-0785-y

Cited by 61 publications

(46 citation statements)

References 19 publications

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“…It is noteworthy that the production rate achieved with AM1-d ( P = 53.19 mg of 2-PE/L/h) is even higher than the rate recently reported for the genetically engineered S. cerevisiae SPO810 strain, which produced 2.61 g/L of 2-PE after 60 h ( P = 43.50 mg of 2-PE/L/h); however, this was in a fed-batch process [Yin et al, 2015]. While in a batch culture in a shaking flask the SPO810 strain produced only 1.10 g/L of 2-PE.…”

Section: Selection Of Diploid S Cerevisiae Am1-d and Testing For 2-pmentioning

confidence: 51%

“…Recent reports show that both de novo synthesis and the production of 2-PE via L -Phe biotransformation can be significantly increased by applying metabolic engineering [B. ; T.-Y. Romagnoli et al, 2015;Yin et al, 2015]. However, because of complicated legislation and negative consumer attitude to genetically modified organisms (GMO), non-GMO strategies are highly recommended and have previously been successfully applied to industrial yeast strains, including the improvement of ethanol tolerance [Stanley et al, 2010], copper resistance [Adamo et al, 2012], or aroma production [Steensels et al, 2014].…”

Section: Introductionmentioning

confidence: 99%

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Mating of 2 Laboratory Saccharomyces cerevisiae Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of <smlcap>L</smlcap>-Phenylalanine

Mierzejewska

Tymoszewska²,

Chreptowicz³

et al. 2017

Microb Physiol

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2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which is widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, which can produce 2-PE by biosynthesis and biotransformation. Thus, the first challenging goal in the development of biotechnological production of 2-PE is searching for highly productive yeast strains. In the present work, 5 laboratory Saccharomyces cerevisiae strains were tested for the production of 2-PE. Thereafter, 2 of them were hybridized by a mating procedure and, as a result, a new diploid, S. cerevisiae AM1-d, was selected. Within the 72-h batch culture in a medium containing 5 g/L of L-phenylalanine, AM1-d produced 3.83 g/L of 2-PE in a shaking flask. In this way, we managed to select the diploid S. cerevisiae AM1-d strain, showing a 3- and 5-fold increase in 2-PE production in comparison to parental strains. Remarkably, the enhanced production of 2-PE by the hybrid of 2 yeast laboratory strains is demonstrated here for the first time.

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Section: Selection Of Diploid S Cerevisiae Am1-d and Testing For 2-pmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Mating of 2 Laboratory Saccharomyces cerevisiae Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of <smlcap>L</smlcap>-Phenylalanine

Mierzejewska

Tymoszewska²,

Chreptowicz³

et al. 2017

Microb Physiol

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“…The presence of specific nitrogen sources, including ammonia, may regulate both the Ehrlich and Shikimate pathways in a number of places. For instance, ARO8 and ARO10 are two key genes in the Ehrlich pathway and their expression has been shown to influence 2PE production30 and to be repressed by ammonia, resulting in lower 2PE production 29…”

Section: Resultsmentioning

confidence: 99%

Elevated production of the aromatic fragrance molecule, 2‐phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes

Chantasuban

Santomauro

Gore‐Lloyd

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND2‐phenylethanol (2PE) is a fragrance molecule predominantly used in perfumes and the food industry. It can be made from petrochemicals inexpensively, however, this is unsuitable for most food applications. Currently, the main method of production for the bio‐derived compound is to extract the trace amounts found in rose petals, which is extremely costly. Potentially fermentation could provide an inexpensive, naturally sourced, alternative.RESULTSIn this investigation, 2PE was produced from the yeast Metschnikowia pulcherrima, optimised in flasks before scaling to 2 L batch and continuous operation. 2PE can be produced in high titres under de novo process conditions with up to 1500 mg L−1 achieved in a 2 L stirred bioreactor. This is the highest reported de novo titre to date, and achieved through high sugar loadings coupled with low nitrogen conditions. The process successfully ran in continuous mode also, with a concentration of 650 mg L−1 of 2PE being maintained. The 2PE production was further increased by the ex novo conversion of phenylalanine and semi‐continuous solid phase extraction from the supernatant. Under optimal conditions 14 000 mg L−1 of 2PE was produced.CONCLUSIONSThe work presented here offers a novel route to naturally sourced 2PE through a scalable fermentation with a robust yeast highly suited to industrial biotechnology. © 2018 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…Another strategy is transamination of l -phenylalanine to phenylpyruvate by aminotransferase. Several groups have demonstrated the biosynthesis of 2-phenylethanol from l -phenylalanine with a high titer by transamination (Kim et al 2014; Yin et al 2015). …”

Section: Resultsmentioning

confidence: 99%

Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine

et al. 2017

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Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis. We establish a microbial biosynthetic pathway for PAA production from the simple substrate phenylalanine in E. coli with heterologous coexpression of aminotransferase (ARO8), keto acid decarboxylase (KDC) and aldehyde dehydrogenase H (AldH) gene. It was found that PAA transformation yield was up to ~94% from phenylalanine in E. coli and there was no by-product phenethyl alcohol was detected. Our results reveal the high efficiency of the RARE strain for production of PAA and indicate the potential industrial applicability of this microbial platform for PAA biosynthesis.

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Improving 2-Phenylethanol Production via Ehrlich Pathway Using Genetic Engineered Saccharomyces cerevisiae Strains

Cited by 61 publications

References 19 publications

Mating of 2 Laboratory <b><i>Saccharomyces cerevisiae</i></b> Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of <smlcap>L</smlcap>-Phenylalanine

Mating of 2 Laboratory <b><i>Saccharomyces cerevisiae</i></b> Strains Resulted in Enhanced Production of 2-Phenylethanol by Biotransformation of <smlcap>L</smlcap>-Phenylalanine

Elevated production of the aromatic fragrance molecule, 2‐phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes

Metabolic engineering of Escherichia coli to high efficient synthesis phenylacetic acid from phenylalanine

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