Improving AOX1 promoter efficiency by overexpression of Mit1 transcription factor

Poodeh, Samin Haghighi; Siadat, Seyed Omid Ranaei; Arjmand, Sareh; Soltani, Maryam Khalifeh

doi:10.1007/s11033-022-07790-7

Cited by 9 publications

(2 citation statements)

References 25 publications

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“…The selected genes and their main function are listed in Table 1 . The increasing trend of methanol-induced transcription factor (Mit-1) expression, which has a crucial role in methanol metabolism and was studied in this group's previous work, was checked to verify the obtained results 65 .…”

Section: Methodsmentioning

confidence: 96%

Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Kabarkouhi

Arjmand

Siadat

et al. 2023

Sci Rep

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Cold atmospheric pressure plasma (CAP) has been described as a novel technology with expanding applications in biomedicine and biotechnology. In the present study, we provide a mildly stressful condition using non-lethal doses of CAP (120, 180, and 240 s) and evaluate its potential benefits on the recombinant production of a model protein (enhanced green fluorescent protein (eGFP)) in yeast Pichia pastoris. The measured eGFP fluorescence augmented proportional to CAP exposure time. After 240 s treatment with CAP, the measured fluorescent intensity of culture supernatant (after 72 h) and results of real-time PCR (after 24 h) indicated an 84% and 76% increase in activity and related RNA concentration, respectively. Real-time analysis of a list of genes involved in oxidative stress response revealed a significant and durable improvement in their expression at five h and 24 h following CAP exposure. The improvement of the recombinant model protein production may be partly explained by the impact of the RONS on cellular constituents and altering the expression of specific stress genes. In conclusion, using CAP strategy may be considered a valuable strategy to improve recombinant protein production, and deciphering the molecular background mechanism could be inspiring in the reverse metabolic engineering of host cells.

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Section: Methodsmentioning

confidence: 96%

Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Kabarkouhi

Arjmand

Siadat

et al. 2023

Sci Rep

Self Cite

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“…At this point, Prm1 activates its own expression and the expression of Mit1, thus inducing strong activation of P AOX1 . The strength of P AOX1 can be improved via Mit1 overexpression [36]; however, Mit1 exerts feedback inhibition of Prm1 expression (Figure 3B) [37]. In consequence, the expression level of Mit1 needs to be delicately tuned to achieve the optimal expression of genes controlled by P AOX1 .…”

Section: Aox1 Promotermentioning

confidence: 99%

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Zha,

Liu,

Ren

et al. 2023

JoF

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Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products. In this review, the general physiological properties of P. pastoris are summarized and the readily available genetic tools and elements are described, including strains, expression vectors, promoters, gene editing technology mediated by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9, and adaptive laboratory evolution. Moreover, the recent achievements in P. pastoris-based biosynthesis of proteins, natural products, and other compounds are highlighted. The existing issues and possible solutions are also discussed for the construction of efficient P. pastoris cell factories.

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Promoters in Pichia pastoris: A Toolbox for Fine-Tuned Gene Expression

Arjmand

2024

Methods in Molecular Biology

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Improving AOX1 promoter efficiency by overexpression of Mit1 transcription factor

Cited by 9 publications

References 25 publications

Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Promoters in Pichia pastoris: A Toolbox for Fine-Tuned Gene Expression

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