Improving endothelialization by the combined application of polyethylene glycol coated cerium oxide nanoparticles and VEGF in electrospun polyurethane scaffolds

Dai, Weiwei; Guo, Hongfeng; Qian, Dehui; Qin, Zhexue; Yan, Lei; Hou, Xiao-Yu; Wen, Can

doi:10.1039/c6tb02391f

Cited by 27 publications

(11 citation statements)

References 42 publications

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“…VEGF immobilized to polyurethane (PU) via polyethylene glycol as a spacer molecule selectively induced EC adhesion and proliferation without affecting platelet adhesion [ 81 ]. Likewise, PEG-coated cerium oxide nanoparticles, which have the potential to inhibit oxidative stress-induced EC apoptosis, and free VEGF embedded in polyurethane scaffolds, facilitated endothelialization [ 82 ]. A similar approach is based on the linkage of plasmids encoding growth factors leading to cell transfection and increased local expression of the respective protein.…”

Section: Strategies To Generate or Mimic Endothelial Basement Membranesmentioning

confidence: 99%

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Lau

Gossen

Lendlein

2021

IJMS

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Insufficient endothelialization of cardiovascular grafts is a major hurdle in vascular surgery and regenerative medicine, bearing a risk for early graft thrombosis. Neither of the numerous strategies pursued to solve these problems were conclusive. Endothelialization is regulated by the endothelial basement membrane (EBM), a highly specialized part of the vascular extracellular matrix. Thus, a detailed understanding of the structure–function interrelations of the EBM components is fundamental for designing biomimetic materials aiming to mimic EBM functions. In this review, a detailed description of the structure and functions of the EBM are provided, including the luminal and abluminal interactions with adjacent cell types, such as vascular smooth muscle cells. Moreover, in vivo as well as in vitro strategies to build or renew EBM are summarized and critically discussed. The spectrum of methods includes vessel decellularization and implant biofunctionalization strategies as well as tissue engineering-based approaches and bioprinting. Finally, the limitations of these methods are highlighted, and future directions are suggested to help improve future design strategies for EBM-inspired materials in the cardiovascular field.

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Section: Strategies To Generate or Mimic Endothelial Basement Membranesmentioning

confidence: 99%

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Lau

Gossen

Lendlein

2021

IJMS

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“…The electrospinning process operated easily and exibly, so this technique was widely applied in forming polymers, including poly(lactic acid) 22,23−25 , poly(glycolic acid) 22,26,27 , poly(lactic-co-glycolic acid) 22,28−30 , polyurethane [31][32][33] , and polycaprolactone [34][35][36][37][38][39] into nano-meter sized materials. Electrospinning process required some operating variables, such as ow rate, applied voltage, spinning time, and distance between collector plate and spray nozzle, which could vary the electrospun materials' traits, including speci c surface area, porosity, mechanical strength, and morphology.…”

Section: Experiments and Methodologymentioning

confidence: 99%

Preparation of Polyimide/Polyethersulfone high temperature resistant nano-fibers for removal of particulate matter

Chen

Lai

2022

Preprint

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Taguchi method was used to determine factors influence, while four factors included ratio of PI and PES, flow rate, voltage, and spinning time, for testing filter efficiency of electrospinning fiber. Spinning time and flow rate were arranged as the top two influenced factors via Taguchi method in this study. Considering test of spinning time, spinning time of 20 min was defined as the best condition to generate the fibrous membrane with filter efficiency and quality of 99.54% and 5.56. Concerning test of flow rate, flow rate of 0.15 ml/hr was the best condition to form the fibrous membrane with filter efficiency and quality of 93.84% and 0.88. This study showed the best conditions of filter efficiency and quality that valued as 93.82% and 2.90 while the most optimal conditions presented ratio of PI and PES (50:50), flow rate (0.15 ml/hr), voltage (22.5 kV), and spinning time (20 mins).Thermogravimetry and differential scanning calorimetry were used to conduct thermal analysis of nano-fiber membrane, and results depicted decomposed temperature of PI and PES were above 500 and 400 ℃. Furthermore, temperature of hot gas filiation usually occurred at the temperature of above 260 ℃, such as 200–350 ℃ for waste incineration, 150–300 ℃ for concrete producing, 130–150 ℃ for thermal power plant operation, and 171–210 ℃ for crematorium operation, so the PI/PES acquired potential advantage on application of hot gas filiation because PI/PES membrane required not only high temperature resistance but also high filter efficiency and quality.

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“…Their biofunctions appear to be critical to repairing endothelium in atherosclerosis and achieving rapid endothelialization of vascular impants. 19,20 In detail, the binding of VEGFs and vascular endothelial growth factor receptor 2 (VEGFR2, a predominant signal transduction receptor of VEGF in the process of angiogenesis and mitosis) can activate the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signal pathways, finally leading to a series of subsequent cascade biochemistry reactions for endothelium recovery.…”

Section: Therapeutic Agents For Repairing Endotheliummentioning

confidence: 99%

Recent advances in inhibiting atherosclerosis and restenosis: from pathogenic factors, therapeutic molecules to nano-delivery strategies

2022

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Improving endothelialization by the combined application of polyethylene glycol coated cerium oxide nanoparticles and VEGF in electrospun polyurethane scaffolds

Abstract: CNPs-PEG/VEGF loaded electrospun PU scaffolds inhibit oxidative stress-induced EPC apoptosis as well as promote endothelialization in vitro.

Cited by 27 publications

References 42 publications

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Preparation of Polyimide/Polyethersulfone high temperature resistant nano-fibers for removal of particulate matter

Recent advances in inhibiting atherosclerosis and restenosis: from pathogenic factors, therapeutic molecules to nano-delivery strategies

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