Improving enzymatic polyurethane hydrolysis by tuning enzyme sorption

Gamerith, Caroline; Acero, Enrique Herrero; Pellis, Alessandro; Ortner, Andreas; Vielnascher, Robert; Luschnig, Daniel; Zartl, Barbara; Haernvall, Karolina; Zitzenbacher, Sabine; Strohmeier, Gernot A.; Hoff, Oskar; Steinkellner, Georg; Gruber, Karl; Ribitsch, Doris; Guebitz, Georg M.

doi:10.1016/j.polymdegradstab.2016.02.025

Cited by 106 publications

(56 citation statements)

References 59 publications

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“…Recently, various strategies for the improvement of such enzymes hydrolysing polyethylene terephthalate (PET) related to thermostability (Miyakawa et al, ; Shirke et al, ; Then et al, ), ultrasound activation (Pellis et al, ), and active site architecture (Araujo et al, ; Silva et al, ; Wei et al, ) were reported. In addition, we have demonstrated that the attachment of hydrophobic binding modules (Gamerith et al, ; Ribitsch et al, ) or hydrophobins to cutinases (Ribitsch et al, ) or enzyme surface engineering (Herrero Acero et al, ) can tune sorption properties and thereby improve PET hydrolysis .…”

Section: Introductionmentioning

confidence: 99%

Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica

Ribitsch

Hromic

Zitzenbacher

et al. 2017

Biotech & Bioengineering

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We have investigated the structures of two native cutinases from Thermobifida cellulosilytica, namely Thc_Cut1 and Thc_Cut2 as well as of two variants, Thc_Cut2_DM (Thc_Cut2_ Arg29Asn_Ala30Val) and Thc_Cut2_TM (Thc_Cut2_Arg19Ser_Arg29Asn_Ala30Val). The four enzymes showed different activities towards the aliphatic polyester poly(lactic acid) (PLLA). The crystal structures of the four enzymes were successfully solved and in combination with Small Angle X-Ray Scattering (SAXS) the structural features responsible for the selectivity difference were elucidated. Analysis of the crystal structures did not indicate significant conformational differences among the different cutinases. However, the distinctive SAXS scattering data collected from the enzymes in solution indicated a remarkable surface charge difference. The difference in the electrostatic and hydrophobic surface properties could explain potential alternative binding modes of the four cutinases on PLLA explaining their distinct activities. Biotechnol. Bioeng. 2017;114: 2481-2488. © 2017 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica

Ribitsch

Hromic

Zitzenbacher

et al. 2017

Biotech & Bioengineering

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“…Properties of the polyols and thus the properties of polyurethanes vary depending on the number of hydroxyl groups, the molar mass and the percentage of groups derived from ethylene oxide. A wide variety of substrates generate lots of material types of very diversified properties [ 15 ].…”

Section: Polyurethanesmentioning

confidence: 99%

Polyurethane Recycling and Disposal: Methods and Prospects

2020

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Growing water and land pollution, the possibility of exhaustion of raw materials and resistance of plastics to physical and chemical factors results in increasing importance of synthetic polymers waste recycling, recovery and environmentally friendly ways of disposal. Polyurethanes (PU) are a family of versatile synthetic polymers with highly diverse applications. They are class of polymers derived from the condensation of polyisocyanates and polyalcohols. This paper reports the latest developments in the field of polyurethane disposal, recycling and recovery. Various methods tested and applied in recent years have proven that the processing of PU waste can be economically and ecologically beneficial. At the moment mechanical recycling and glycolysis are the most important ones. Polyurethanes’ biological degradation is highly promising for both post-consumer and postproduction waste. It can also be applied in bioremediation of water and soil contaminated with polyurethanes. Another possibility for biological methods is the synthesis of PU materials sensitive to biological degradation. In conclusion, a high diversity of polyurethane waste types and derivation results in demand for a wide range of methods of processing. Furthermore, already existing ones appear to be enough to state that the elimination of not reprocessed polyurethane waste in the future is possible.

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“…Over the last three decades, several research groups have isolated microorganisms capable of attacking PU (Oceguera-Cervantes et al, 2007;Cregut et al, 2013;Álvarez-Barragán et al, 2016;Gamerith et al, 2016;Osman et al, 2018;Magnin et al, 2019) and degrading xenobiotic additives (Ojo, 2007;Varsha et al, 2011). Also, the degradation capabilities of several fungal and bacterial communities over PU in compost, soil or liquid cultures (Zafar et al, 2014;Shah et al, 2016;Vargas-Suárez et al, 2019), and over some xenobiotics in different activated sludges (Ferrero et al, 2018) have been assessed.…”

Section: Introductionmentioning

confidence: 99%

Degradation of Recalcitrant Polyurethane and Xenobiotic Additives by a Selected Landfill Microbial Community and Its Biodegradative Potential Revealed by Proximity Ligation-Based Metagenomic Analysis

et al. 2020

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Polyurethanes (PU) are the sixth most produced plastics with around 18-million tons in 2016, but since they are not recyclable, they are burned or landfilled, generating damage to human health and ecosystems. To elucidate the mechanisms that landfill microbial communities perform to attack recalcitrant PU plastics, we studied the degradative activity of a mixed microbial culture, selected from a municipal landfill by its capability to grow in a water PU dispersion (WPUD) as the only carbon source, as a model for the BP8 landfill microbial community. The WPUD contains a polyether-polyurethane-acrylate (PE-PU-A) copolymer and xenobiotic additives (N-methylpyrrolidone, isopropanol and glycol ethers). To identify the changes that the BP8 microbial community culture generates to the WPUD additives and copolymer, we performed chemical and physical analyses of the biodegradation process during 25 days of cultivation. These analyses included Nuclear magnetic resonance, Fourier transform infrared spectroscopy, Thermogravimetry, Differential scanning calorimetry, Gel permeation chromatography, and Gas chromatography coupled to mass spectrometry techniques. Moreover, for revealing the BP8 community structure and its genetically encoded potential biodegradative capability we also performed a proximity ligation-based metagenomic analysis. The additives present in the WPUD were consumed early whereas the copolymer was cleaved throughout the 25-days of incubation. The analysis of the biodegradation process and the identified biodegradation products showed that BP8 cleaves esters, CC , and the recalcitrant aromatic urethanes and ether groups by hydrolytic and oxidative mechanisms, both in the soft and the hard segments of the copolymer. The proximity ligation-based metagenomic analysis allowed the reconstruction of five genomes, three of them from novel species. In the metagenome,

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Improving enzymatic polyurethane hydrolysis by tuning enzyme sorption

Cited by 106 publications

References 59 publications

Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica

Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica

Polyurethane Recycling and Disposal: Methods and Prospects

Degradation of Recalcitrant Polyurethane and Xenobiotic Additives by a Selected Landfill Microbial Community and Its Biodegradative Potential Revealed by Proximity Ligation-Based Metagenomic Analysis

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