Improving fat graft survival through preconditioning of the recipient site with microneedling

Sezgin, Billur; Özmen, Selahattin; Bulam, Hakan; Ömeroğlu, Suna; Yüksel, Seher; Çayci, Banu; Peker, Tuncay

doi:10.1016/j.bjps.2014.01.019

Cited by 47 publications

(25 citation statements)

References 44 publications

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“…6 and 7). These observations are also in agreement with the findings of previous studies, demonstrating a link between neovascularization and improved fat graft survival (8,16,35). …”

Section: Discussionsupporting

confidence: 93%

Bone marrow mesenchymal stem cell-derived extracellular vesicles improve the survival of transplanted fat grafts

Huang

Feng

Zhang

et al. 2017

Molecular Medicine Reports

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Autologous fat grafting is a promising surgical technique for soft tissue augmentation, reconstruction and rejuvenation. However, it is limited by the low survival rate of the transplanted fat, due to the slow revascularization of such grafts. Previous studies have demonstrated that bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSC-EVs) are proangiogenic. The present study aimed to investigate whether BMSC-EVs could improve the survival of transplanted fat grafts. Extracellular vesicles were isolated from the supernatant of cultured rat bone marrow mesenchymal stem cells, and characterized by flow cytometry and scanning electron microscopy. Their proangiogenic potential was measured in vitro using tube formation and cell migration assays. Subsequently, human fat tissue grafts, alongside various concentrations of BMSC-EVs, were subcutaneously injected into nude mice. A total of 12 weeks following transplantation, the mice were sacrificed and the grafts were harvested. The grafts from the experimental group had a higher survival rate and an increased number of vessels compared with grafts from the control group, as demonstrated by tissue volume, weight and histological analyses. Reverse transcription-quantitative polymerase chain reaction analysis indicated that the expression levels of proangiogenic factors were increased in the experimental group compared with in the control group, thus suggesting that BMSC-EVs may promote neovascularization by stimulating the secretion of proangiogenic factors. The present study is the first, to the best of our knowledge, to demonstrate that supplementation of fat grafts with BMSC-EVs improves the long-term retention and quality of transplanted fat.

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“…6 and 7). These observations are also in agreement with the findings of previous studies, demonstrating a link between neovascularization and improved fat graft survival (8,16,35). …”

Section: Discussionsupporting

confidence: 93%

Bone marrow mesenchymal stem cell-derived extracellular vesicles improve the survival of transplanted fat grafts

Huang

Feng

Zhang

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

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“…There have been studies supporting microneedling [38]. They mentioned that there was more vascularity, higher graft survival, and better graft integrity with less fibrosis after preconditioning with microneedling 1 week before grafting [39]. …”

Section: Resultsmentioning

confidence: 99%

Modern trends in lipomodeling

El-Sabbagh

2017

GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW; 6:Doc06

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“…Transplanted fat is vascularized in a centripetal manner, with an ability to survive by plasmatic imbibition for a distance ranging from 0.5 to 1.5 mm. Therefore, the maximum diameter of a free fat transplant must be 3 mm. For this reason, we chose to avoid large grafts, which would result in unaesthetic overcorrection and considerable resorption because of lack of nutrition.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of autologous fat vs hyaluronic acid in correction of the nasolabial folds

Xue

et al. 2017

J of Cosmetic Dermatology

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Improving fat graft survival through preconditioning of the recipient site with microneedling

Cited by 47 publications

References 44 publications

Bone marrow mesenchymal stem cell-derived extracellular vesicles improve the survival of transplanted fat grafts

Bone marrow mesenchymal stem cell-derived extracellular vesicles improve the survival of transplanted fat grafts

Modern trends in lipomodeling

Comparative study of autologous fat vs hyaluronic acid in correction of the nasolabial folds

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