Improving reproducibility between batches of silver nanoparticles using an experimental design approach

Abstract

Improving reproducibility between batches of silver nanoparticles using an experimental design approach.

Optimization of silver nanoparticle synthesis by chemical reduction and evaluation of its antimicrobial and toxic activity

“…There are several studies that optimize the synthesis parameters of AgNPs, mainly to reduce the size and improve their physico-chemical properties [14, 15]. However, even if there are well-established techniques for the preparation of metallic nanoparticles, it is necessary to investigate simple synthesis methods, which require short reaction times and low cost to obtain nanoparticles with greater antimicrobial activity [16].…”

Controlled synthesis of SPION@SiO2nanoparticles using design of experiments

“…Despite their identification as important experimental conditions in the formation of SPION@SiO 2 NPs, these parameters have not been investigated through a DoE approach, which allows for the simultaneous variation of experimental factors. 72,76–78 This not only enables the evaluation of interactions between these factors, but also reduces the impact of random variation. Furthermore, by implementing 3 levels (low, centre, high), non-linear effects can also be measured.…”

“…71 There have been several successful examples of the optimisation of nanoparticle synthesis and maximising their functionality using a DoE approach in the literature. 23,72–80…”

MultiShapeC, an algorithm to assess concentration in multi-shape nanoparticle samples: nanosilver, a case study

“…In order to evaluate the impact of using MultiShapeC vs. the average size method for the NP concentration calculation, we studied the interaction between AgNP and carbazole by fluorescence quenching. 40 Different aliquots of AgNR–AgNS (0–1.35 nM) were mixed with a fixed concentration of carbazole (100 nM), and the fluorescence emission spectra of the mixtures were recorded between 350 nm and 600 nm. Fluorescence intensities were corrected as in our previous work by considering inner filter effects.…”

“…The accurate determination of their concentration is one of the most critical parameters, mainly because nanoparticles (NP) are frequently obtained with a certain degree of polydispersity, 39 indeed when it is possible to get highly uniform NP, for example, nanospheres. 40,41 A few publications discuss this major challenge in nanoscience, focusing on polydispersity. 42,43 However, the calculation of NP concentration in multi-shape samples remains generally underestimated due to overlooking each shape's contribution to the total NP concentration.…”