Improving the Analytical Performances of Inductively Coupled Plasma Optical Emission Spectrometry by Multivariate Analysis Techniques

Grotti, Marco

doi:10.1002/adic.200490011

Cited by 15 publications

(9 citation statements)

References 108 publications

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“…This activity is sensitive to heat, proteases, sodium dodecyl sulfate (SDS), and guanidine hydrochloride, arguing that it is due to the presence of an enzyme. We confirmed that the product of tellurite reduction by the S. epidermidis CH extract is tellurium by using Induced Coupled Plasma-Optical Emission (ICP-OE) spectroscopy [30]. We compared the spectrum of the product produced by the extract with the spectra of metallic tellurium and potassium tellurite reduced chemically with 2-mercaptoethanol, as standards.…”

Section: Resultsmentioning

confidence: 68%

“…Absorption spectra of products formed upon the reduction of K 2 TeO 3 in vitro by (A) 2-mercaptoethanol, (B) a crude extract prepared from S. epidermidis CH cells prior to chromatographic enrichment, and (C) purified bovine liver catalase, were resolved by Induced Coupled Plasma-Optical Emission spectroscopy [30].…”

Section: Resultsmentioning

confidence: 99%

“…Mixes were centrifuged at 10,000× g for 10 min at 25°C, pellets were washed three times each with 0.1 M NaCl and distilled deionized water. The black pellets were dissolved in a solution of 10% bromine, and analyzed by Induced Coupled Plasma-Optical Emission (ICP-OE) spectroscopy [30], using an Optima 2000 DV Perkin-Elmer apparatus equipped with a Model 730 autosampler. Standards for this analysis were metallic tellurium, and the product obtained upon the chemical reduction of potassium tellurite with an excess of 2-mercaptoethanol; these were processed in parallel with the products of enzyme-catalyzed reactions.…”

Section: Methodsmentioning

confidence: 99%

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Catalases Are NAD(P)H-Dependent Tellurite Reductases

et al. 2006

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Reactive oxygen species damage intracellular targets and are implicated in cancer, genetic disease, mutagenesis, and aging. Catalases are among the key enzymatic defenses against one of the most physiologically abundant reactive oxygen species, hydrogen peroxide. The well-studied, heme-dependent catalases accelerate the rate of the dismutation of peroxide to molecular oxygen and water with near kinetic perfection. Many catalases also bind the cofactors NADPH and NADH tenaciously, but, surprisingly, NAD(P)H is not required for their dismutase activity. Although NAD(P)H protects bovine catalase against oxidative damage by its peroxide substrate, the catalytic role of the nicotinamide cofactor in the function of this enzyme has remained a biochemical mystery to date. Anions formed by heavy metal oxides are among the most highly reactive, natural oxidizing agents. Here, we show that a natural isolate of Staphylococcus epidermidis resistant to tellurite detoxifies this anion thanks to a novel activity of its catalase, and that a subset of both bacterial and mammalian catalases carry out the NAD(P)H-dependent reduction of soluble tellurite ion (TeO3 2−) to the less toxic, insoluble metal, tellurium (Te°), in vitro. An Escherichia coli mutant defective in the KatG catalase/peroxidase is sensitive to tellurite, and expression of the S. epidermidis catalase gene in a heterologous E. coli host confers increased resistance to tellurite as well as to hydrogen peroxide in vivo, arguing that S. epidermidis catalase provides a physiological line of defense against both of these strong oxidizing agents. Kinetic studies reveal that bovine catalase reduces tellurite with a low Michaelis-Menten constant, a result suggesting that tellurite is among the natural substrates of this enzyme. The reduction of tellurite by bovine catalase occurs at the expense of producing the highly reactive superoxide radical.

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Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Catalases Are NAD(P)H-Dependent Tellurite Reductases

et al. 2006

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“…These approaches have been used successfully in chemical analysis and research efforts have continuously been made to improve the accuracy and reliability of the output. [4][5][6] Chemical analysis using array sensing approach mainly includes electrochemical [7][8][9], mass [10,10] and optical sensors [12][13][14][15][16][17][18]. In the electrochemical sensing category a large proportion of the work has been in the area of gas sensing that is based on conductometric techniques.…”

Section: Introductionmentioning

confidence: 99%

Quantitative colorimetric analysis of dye mixtures using an optical photometer based on LED array

Lau

Yerazunis

Shepherd

et al. 2006

Sensors and Actuators B: Chemical

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“…For each digestion step, a blank was prepared with the same amount of acids. [17][18][19][20] The digestion programme for microwave system was: 3 min at 250 W, 2 min at 0 W, 5 min at 250 W, 5 min at 500 W, 2 min at 0 W and 5 min at 500 W. After digestion, samples were diluted to 50 mL with high purity water. The resulting solutions were analyzed by ICP-AES technique for Cd, Cr, Cu, Fe, Mn, Ni, Pb, V and Zn determination.…”

Section: Pm10 and Metals Determinationmentioning

confidence: 99%

Heavy Metals in PM10 Sampled in the Urban Area of Campi Salentina (Apulia, Southern Italy)

Buccolieri

Cardellicchio

et al. 2006

Annali di Chimica

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The following paper shows results of PM10 level and Cd, Cr, Cu, Fe, Mn, Ni, Pb, V and Zn concentrations present in this fraction for filters collected in the urban area of Campi Salentina (Apulia, Southern Italy) in 2004 in order to inquire into air quality. PM10 and lead concentrations did not exceed the standard values sanctioned by Italian Legislature with average values equal to 36.54 +/- 14.57 microg x m(-3) and 9.19 +/- 5.24 ng x m(-3) respectively. Significant correlations have been found between the pairs Fe-Mn, Ni-V, Cr-Ni and Cd-Cr. This suggests that these metals have a similar pollution source probably due to metallurgical activity. Multivariate statistical analysis showed that it is possible to distinguish the PM10 samples collected in sampling periods with different meteorological conditions. Indeed, metals concentration increases for samples characterized by scarce rains or by winds prevailing from North or North-West and is reduced due to different meteorological conditions, although there are some exceptions.

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Improving the Analytical Performances of Inductively Coupled Plasma Optical Emission Spectrometry by Multivariate Analysis Techniques

Cited by 15 publications

References 108 publications

Catalases Are NAD(P)H-Dependent Tellurite Reductases

Catalases Are NAD(P)H-Dependent Tellurite Reductases

Quantitative colorimetric analysis of dye mixtures using an optical photometer based on LED array

Heavy Metals in PM10 Sampled in the Urban Area of Campi Salentina (Apulia, Southern Italy)

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