Improving the sensitivity of the determination of ceftiofur by capillary electrophoresis in environmental water samples: In-line solid phase extraction and sample stacking techniques

Puig, Patricia; Borrull, Francesc; Calull, Marta; Benavente, Fernando; Sanz‐Nebot, Victoria; Barbosa, J.; Aguilar, Carme

doi:10.1016/j.aca.2007.01.043

Cited by 41 publications

(51 citation statements)

References 33 publications

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“…On-line or in-line coupling of the preconcentration with CE has also become a recent trend [2]. The use of a preconcentrating device at the head of the capillary has been explored in a number of recent works [66,68,70,72]. For example, Zhang et al [68] developed an in-capillary SPE-CE method by incorporating a 0.5 mm-long SPE sorbent into the inlet end of a capillary for analysis of chlorophenols in water [68].…”

Section: Sample Preconcentrationmentioning

confidence: 98%

“…In recent years, the presence of pharmaceuticals in surface water has become a topic of increasing interest [26,49,65,70,92]. A number of excellent reviews have recently been published on this subject [6,13,14,16].…”

Section: Drug Residuesmentioning

confidence: 99%

“…Many novel chromatographic and electrophoretic preconcentration techniques, as well as combinations of these procedures, have been recently applied [26,34,49,[64][65][66][67][68][69][70][71][72]. Off-line sample cleanup and preconcentration have been employed through the use of SPE [26,32,34,49,50,64,70], stir bar sorption extraction (SBSE) [67], polymer-coated hollow fiber microextraction [65], and liquid-liquid semi-microextraction (LLsME) [71].…”

Section: Sample Preconcentrationmentioning

confidence: 99%

“…This is afforded by manipulating the composition and ionic strength of the sample matrix. Normal stacking [65], field-amplified injection (FASI) [38,64,73,74], large volume sample stacking (LVSS) [26,70,75], ITP [66,76], high salt stacking [77], and sweeping [63,73] were the most frequently used techniques in the reviewed period. FASI (in some cases termed field-enhancement sample injection (FESI)) has proven to be a very effective in-line preconcentration technique.…”

Section: Sample Preconcentrationmentioning

confidence: 99%

“…When the sample matrix is almost completely removed from the capil-lary (the current is about 97-99% of the predetermined current), the polarity is once again reversed and the separation is performed. This methodology was used in the determination of copper carboxylates [75], sulfoamides [26], and ceftiofur [70] in various environmental water samples. In the latter work, LVSS and on-line SPE-CE methods were compared, showing that while LVSS allowed for a faster and more reproducible analysis with higher peak efficiency, on-line SPE-CE allowed for enhanced sensitivity with a LOD of 250 ng/L ceftiofur in standard solutions.…”

Section: Sample Preconcentrationmentioning

confidence: 99%

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Recent advances in CE and CEC of pollutants

Da̧bek-Złotorzyńska¹,

Celo²,

Yassine³

2007

Electrophoresis

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Recent advances in CE and CEC separation, detection and sample preparation/preconcentration methodologies, for the determination of a variety of compounds having current or potential environmental relevance, have been overviewed. The reviewed literature has illustrated the wide range of CE applications available, indicating a continuing interest in CE and CEC in the environmental field. New developments in chip-based CE systems are also discussed.

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Section: Sample Preconcentrationmentioning

confidence: 98%

Section: Drug Residuesmentioning

confidence: 99%

Section: Sample Preconcentrationmentioning

confidence: 99%

Section: Sample Preconcentrationmentioning

confidence: 99%

Section: Sample Preconcentrationmentioning

confidence: 99%

See 3 more Smart Citations

Recent advances in CE and CEC of pollutants

Da̧bek-Złotorzyńska¹,

Celo²,

Yassine³

2007

Electrophoresis

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Organic Analysis in Environmental Samples by Capillary Electrophoresis

García‐Ruiz

Crego

Marina

2015

Encyclopedia of Analytical Chemistry

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Capillary electrophoresis (CE) is presented as an efficient and potent alternative to other chromatographic techniques, such as liquid chromatography (LC) or gas chromatography (GC), to analyze organic pollutants in environmental samples. Despite the insufficient sensitivity associated with the use of CE with optical detection, the latest developments on the use of alternative detection systems and/or sample preconcentration techniques together with its efficiency and versatility have demonstrated the potential of CE to face the determination of pollutants at trace levels. Other interesting characteristics of CE in the field of environmental analysis include its enormous possibilities to achieve stereoselective analysis of chiral compounds and the low amount of reagents needed (green analytical technique). Nowadays, a large number of research works have been published dealing with the use of CE as a powerful technique for pollutants analysis. This article presents the fundamentals of CE techniques as well as the main developments and applications performed in environmental analysis during the past 10 years. The CE determination of a large variety of organic pollutants, including pesticides, phenols, pharmaceuticals, nitroaromatic compounds, amines, and so on, is described.

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Use of large‐volume sample stacking in on‐line solid‐phase extraction‐capillary electrophoresis for improved sensitivity

et al. 2008

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We present a new system for the sensitive analysis of cephalosporins by CE using both on-line SPE and large-volume sample stacking (LVSS). Sample volumes of 250 muL were loaded onto the SPE microcolumn which was then desorbed with 426 nL of ACN. The SPE elution plug was injected into the CE system via an in-line valve interface filling approximately 60% of the volume of the separation capillary. Subsequently, LVSS was performed by applying a voltage of -5 kV, which resulted in the simultaneous removal of the elution solvent and the preconcentration of the analytes in a narrow zone. This way the amount of analyte loaded into the capillary could be considerably increased without serious loss of CE separation efficiency. LODs for cefoperazone and ceftiofur were in the ng/L range which represents an improvement of a factor of 8450 and 11 450 when compared with direct CE injection. The cephalosporin test compounds presented a good linear response (corrected peak area) between 0.5 and 10 mug/L with correlation coefficients higher than 0.995. The final method is compared with previously reported LVSS-CE and SPE-CE systems for the analysis of cephalosporins.

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Improving the sensitivity of the determination of ceftiofur by capillary electrophoresis in environmental water samples: In-line solid phase extraction and sample stacking techniques

Cited by 41 publications

References 33 publications

Recent advances in CE and CEC of pollutants

Recent advances in CE and CEC of pollutants

Organic Analysis in Environmental Samples by Capillary Electrophoresis

Use of large‐volume sample stacking in on‐line solid‐phase extraction‐capillary electrophoresis for improved sensitivity

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