Improving Transdermal Drug Delivery System for Medroxyprogesterone Acetate by Olive Oil and Dimethylsulfoxide (Dmso) as Penetration Enhancers: In Vitro Penetration Study

Abstract

Objective: Medroxyprogesterone Acetate (MPA) using a transdermal drug delivery system for contraception by passive diffusion is limited by the skin barrier properties. Penetration enhancers such as olive oil (fatty acid permeation enhancer) and DMSO (chemical enhancer) can be used. The objective of this study was to overcome MPA penetration problem by using olive oil and DMSO.
 Methods: An in vitro penetration study using the Franz diffusion cells was performed. The first penetration study used MPA in olive oil (O) and MPA in coconut oil (C) with the concentration 100 μg/ml to each sample and MPA suspension as a control with the same concentration. The second study used MPA in olive oil with the concentration 200.0 μg/ml (A), MPA in olive oil with 0.5% DMSO with the concentration 200.0 μg/ml (B), and MPA in olive oil with 1% DMSO with the concentration 200 μg/ml (C).
 Results: MPA penetration test for olive oil+0.5% DMSO had flux value 4.24±0.074 μg/cm2. hr and it was not significantly different (t-test, P>0.05) with olive oil+1% DMSO. While the MPA penetration test in only Olive oil had flux value 0.90±0.0087 μg/cm2. hr.
 Conclusion: This research concluded that olive oil and 0.5% DMSO could improve the penetration of MPA into skin membrane by 4.5 times more than olive oil alone.

Current Development of Chemical Penetration Enhancers for Transdermal Insulin Delivery

“…In addition, DMSO is known for its ability to transport smaller molecules across the skin and mucous membranes [ 85 , 86 ]. This is largely due to its amphiphilic nature, where DMSO interacts with lipids at the stratum corneum, altering the structure of the peptide, which then changes the partition coefficient and therefore increases the permeability of molecules [ 87 ]. One of the earliest studies on the efficacy of DMSO in enhancing transdermal permeation of 14C-labeled propan-1-ol and hexan-1-ol was performed on the stratum corneum of neonates.…”