Impulse acoustic microscopy: A new approach for investigation of polymer and natural scaffolds

Abstract

The aim of this article is to verify the applicability of impulse acoustic microscopy as a tool for evaluating scaffolds in tissue engineering. Decellularized rat diaphragms and nonwoven polymer scaffolds made from cellulose diacetate, chlorinated polyvinyl chloride, and polysulfone were used as model objects. Optical microscopy, scanning electron microscopy, and histological research were used as reference methods in order to realize feasibility of the acoustic microscopy method in a regenerative medicine field. Direct comparison of different methods was carried out. The high frequency ultrasound microscopy is defined as a useful non‐invasive tool for studying and investigating the bulk morphology and bulk structure of opaque scaffolds, which allows to diagnose and to assume heterogeneous regions and defects inside the specimens. POLYM. ENG. SCI., 57:709–715, 2017. © 2017 Society of Plastics Engineers

The Influence of Microstructure in PHB Non‐Woven Materials on Mechanical Behavior Under Tension

“…Previously, possibility of high‐frequency ultrasound method for visualization of volume microstructure in non‐woven materials was described in. [ 10 ]…”

“…Previously, possibility of high-frequency ultrasound method for visualization of volume microstructure in non-woven materials was described in. [10] In this work, the study of mechanical behavior of electrospun materials of poly(3-hydroxybutyrate) (PHB) with various morphology was conducted. Two manufacturing process have been used for producing four series of non-woven specimens.…”

Noninvasive ultrasound imaging for assessment of intact microstructure of extracellular matrix in tissue engineering

“…Experimental setup of SIAM‐2011 is shown in Figure . The method is described in detail in previous works . We used common regime of imaging—C‐scanning which presents internal microstructure in the form of layer‐by‐layer images of horizontal cross‐sections at different depths (Figure ); and modified B/D‐mode (brightness/depth) which images internal microstructure in vertical section.…”

“…The method is described in detail in previous works. [23][24][25] We used common regime of imaging-C-scanning which 26 which images internal microstructure in vertical section. B/D-scan is archived by dynamic shifting of the probe beam focus position inside the sample volume ( Figure 2B).…”

Определение Структуры Децеллюляризированных Дермальных Матриксов

“…Для достижения большей информативности были получены акустические изображения образов двух типов -B/D-сканы и С-сканы, соответствующие поперечным и продольным срезам кожи при изготовлении срезов во время гистологической обработки (рис. 3) [34,35]. На акустических изображениях нативных образцов (рис.…”