Imunofluorescência direta e indireta

Abstract

Abstract:Immunofluorescence is a valuable auxiliary diagnostic tool for autoimmune bullous diseases and inflammatory disorders, since their clinical and histopathologic findings may be inconclusive. It is a feasible laboratory method that requires experienced technicians and detects in situ and circulating immune deposits that may be involved in the pathogenesis of such skin diseases. Keywords: Autoimmunity; Basement membrane; Fluorescent antibody technique; Pemphigus Resumo: A imunofluorescência é um valioso instrumento auxiliar no diagnóstico das dermatoses bolhosas autoimunes e desordens inflamatórias, uma vez que seus achados clínicos e histopatológicos podem não ser determinantes. Consiste em um método laboratorial factível, que requer profissionais técnicos experientes, e detecta imunocomplexos in situ e/ou circulantes, que podem estar envolvidos na patogênese de tais enfermidades cutâneas.

Case for diagnosis

“…Because the clinical presentation of the disease and the histological findings are often atypical, the most consistent test for diagnosis of PH is immunofluorescence. 3 Direct immunofluorescence (DIF) performed on perilesional skin revealed intraepithelial intercellular IgG deposits, which is the typical finding. 3 Most PH autoantibodies target desmoglein (Dsg) 1, with exceptional cases manifesting autoantibodies against Dsg 3, implicating the predominant autoimmunity similar to pemphigus foliaceus rather than pemphigus vulgaris.…”

Comparative study of direct and indirect immunofluorescence for diagnosis of canine pemphigus foliaceus

“…In human medicine, immunofluorescence assays are widely used for the diagnosis of autoimmune blistering diseases (Aoki et al, 2010). It is a valuable and useful tool, especially when the histopathological examination (gold standard) is inconclusive.…”

“…These aspects of the immunofluorescence can be evidenced in 100% of the human patients with the active disease, when appropriate collection of cutaneous fragment (Rani and Hussain, 2003;Aoki et al, 2010).…”

“…In human medicine, the immunofluorescence techniques have emerged as useful methods for diagnosing autoimmune blistering conditions since the early 1960s and present excellent results (Beutner and Gordon, 1964;Brandsen et al, 1997). Currently, immunofluorescence tests are a useful tool for the laboratory diagnosis of autoimmune dermatosis in humans, since their clinical and histopathological findings may be inconclusive (Mutasim and Adams, 2001;Aoki et al, 2004;Aoki et al, 2010).…”

“…There are two main subtypes of immunofluorescence tests: direct immunofluorescence (DIF), which is performed on perilesional skin or mucous membranes and detects tissue-bound autoantibodies; and indirect immunofluorescence (IIF), which quantifies a patient's autoantibodies circulating in the blood, utilizing canine footpad as substrates (Mutasim and Adams, 2001;Aoki et al, 2010).…”