In‐field volatile analysis employing a hand‐held portable GC‐MS: emission profiles differentiate damaged and undamaged yellow starthistle flower heads

Beck, John J.; Porter, Nathan L.; Cook, Daniel; Gee, Wai S.; Griffith, Corey M.; Rands, Anthony D.; Truong, Tai V.; Smith, Lincoln; Román, I. San

doi:10.1002/pca.2573

Cited by 31 publications

(30 citation statements)

References 27 publications

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“…Hervibore-induced 2-dodecanone has been detected as a tomato volatile blend component 31 . The alkane tetradecane, a known semiochemical for many arthropods 32 , has also been identified as a volatile biomarker indicating damaged flower head tissue 33 . The most remarkable finding of our study was that three aliphatic compounds were mainly detected after an aphid attack in plants grown in the inoculated microbial soil.…”

Section: Discussionmentioning

confidence: 99%

Bottom-up effects on herbivore-induced plant defences: a case study based on compositional patterns of rhizosphere microbial communities

Benítez

Paredes

Rodrı́guez

et al. 2017

Sci Rep

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Below-ground soil microorganisms can modulate above-ground plant-insect interactions. It still needs to be determined whether this is a direct effect of single species or an indirect effect of shifts in soil microbial community assemblages. Evaluation of the soil microbiome as a whole is critical for understanding multi-trophic interactions, including those mediated by volatiles involving plants, herbivorous insects, predators/parasitoids and microorganisms. We implemented a regulated system comprising Nerium oleander plants grown in soil initially containing a sterile/non sterile inoculum, herbivore Aphis nerii and predator Chrysoperla carnea. After aphid attack, plants emitted a characteristic blend of volatiles derived from two biosynthetic classes: fatty acid catabolites and aromatic-derived products. Three aliphatic compounds were mainly detected in plants grown in the inoculated microbial soil, a blend which was preferentially chosen by C. carnea adult females. The contrasting effect of the initial inocula was attributed to the different microbial consortia developed in each treatment. We argue that differences in the relative abundance of the active microbial communities in the rhizosphere correlate with those in the emission of selected volatile compounds by attacked plants. The mechanisms involved in how the functional soil microbiome modulates inducible indirect defence of plants are discussed.

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Section: Discussionmentioning

confidence: 99%

Bottom-up effects on herbivore-induced plant defences: a case study based on compositional patterns of rhizosphere microbial communities

Benítez

Paredes

Rodrı́guez

et al. 2017

Sci Rep

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“…However, GC/DMS has many advantages that make it ideal for these environments. The devices are small and easily transported; they operate under normal atmospheric conditions and temperatures, even without the need for supplemental gases; they can be used in in situ investigations that avoid having to remove plant material from quarantine facilities or elaborate sample preparations; they allow for diagnostic developments that may be adaptable to field studies for real-time disease analysis; GC/DMS has been in increasing demand for biological applications (13 (19) (20), which may be easily installed in such greenhouses, but DMS allows for detection of VOCs not resolved by GC/MS, such as the separation of certain isomers (21). Given the importance of isomers in biological signal transduction pathways, resolving these metabolite compounds is frequently important.…”

Section: Dms Also Known As High Field Asymmetric Waveform Ion Mobilimentioning

confidence: 99%

Coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings

McCartney

Spitulski

Pasamontes

et al. 2016

Talanta

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Volatile organic compounds (VOCs) are off-gassed from all living organisms and represent end products of metabolic pathways within the system. In agricultural systems, these VOCs can provide important information on plant health and can ordinarily be measured non-invasively without harvesting tissue from the plants. Previously we reported a portable gas chromatography/differential mobility spectrometry (GC/DMS) system that could distinguish VOC profiles of pathogen-infected citrus from healthy trees before visual symptoms of disease were present. These measurements were taken directly from canopies in the field, but the sampling and analysis protocol did not readily transfer to a controlled greenhouse study where the ambient background air was saturated with volatiles contained in the facility. In this study, we describe for the first time a branch enclosure uniquely coupled with GC/DMS to isolate and measure plant volatiles. To test our system, we sought to replicate our field experiment within a contained greenhouse and distinguish the VOC profiles of healthy versus citrus infected with Candidatus Liberibacter asiaticus. We indeed confirm the ability to track infection-related trace biogenic VOCs using our sampling system and method and we now show this difference in Lisbon lemons (Citrus×limon L. Burm. f.), a varietal not previously reported. Furthermore, the system differentiates the volatile profiles of Lisbon lemons from Washington navels [Citrus sinensis (L.) Osbeck] and also from Tango mandarins (Citrus reticulata Blanco). Based on this evidence, we believe this enclosure-GC/DMS system is adaptable to other volatile-based investigations of plant diseases in greenhouses or other contained settings, and this system may be helpful for basic science research studies of infection mechanisms.

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“…The technique of volatile analysis poses analytical challenges and must be adapted either to laboratory or field experiments. Portable gas chromatography/mass spectrometry (GC/MS) represents the best compromise for studies investigating both biotic and abiotic stresses in field research . The isoprene molecule (C 5 H 8 ) has been extensively studied with real‐time volatile collection methods using proton transfer reaction/mass spectrometry (PTR‐MS) technology .…”

Section: Introductionmentioning

confidence: 99%

“…spectrometry (GC/MS) represents the best compromise for studies investigating both biotic and abiotic stresses in field research. 3 The isoprene molecule (C 5 H 8 ) has been extensively studied with real-time volatile collection methods using proton transfer reaction/mass spectrometry (PTR-MS) technology. 4 Conversely, in vitro headspace analysis in connection with abiotic stress studies is usually performed under laboratory conditions.…”

mentioning

confidence: 99%

A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

Durenne

Blondel

Druart

et al. 2018

Phytochemical Analysis

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IntroductionThe dynamic headspace sampling technique using thermal desorption, gas chromatography‐mass spectrometry (TD‐GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds (VOCs), and experiments performed in sterile and controlled conditions can be useful for VOC metabolism investigations.ObjectiveThe main purpose of this study was to set up a laboratory high‐throughput glass chamber for whole plant volatiles analysis. Brassica napus L. plantlets were tested with the developed system to better understand the relationship between low emission of induced terpene and cadmium (Cd)‐related abiotic stress.MethodologyVOCs emitted by 28‐day‐old Brassica napus L. plantlets cultivated in vitro were trapped with our device using adsorbent cartridges that were desorbed with a thermal desorption unit before cryofocusing with a cooled injection system and programmable temperature vaporising inlet into an HP‐5 ms GC column. Terpene detection and quantitation from chromatogram profiles were acquired using selected ion monitoring (SIM) mode during full scan analysis and mass spectra were obtained with a quadrupole‐type mass spectrometer.ResultsThe new trapping method produced reliable qualitative profiles of oilseed rape VOCs. Typical emissions of monoterpenes (myrcene, limonene) and sesquiterpenes (β‐elemene, (E,E)‐α‐farnesene) were found for the different concentrations tested. One‐way analysis of variance for quantitative results of (E,E)‐α‐farnesene emission rates showed a Cd concentration effect.ConclusionThis inexpensive glass chamber has potential for wide application in laboratory sterile approach and replicated research. Moreover, the non‐invasive dynamic sampling technique could also be used to analyse volatiles under both abiotic and biotic stresses.

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In‐field volatile analysis employing a hand‐held portable GC‐MS: emission profiles differentiate damaged and undamaged yellow starthistle flower heads

Abstract: The portable GC-MS system distinguished the treatments based on their detected volatile profiles. Additional statistical analysis identified five possible biomarker volatiles for the treatments, among them cyclosativene and copaene, which indicated damaged flower heads.

Cited by 31 publications

References 27 publications

Bottom-up effects on herbivore-induced plant defences: a case study based on compositional patterns of rhizosphere microbial communities

Bottom-up effects on herbivore-induced plant defences: a case study based on compositional patterns of rhizosphere microbial communities

Coupling a branch enclosure with differential mobility spectrometry to isolate and measure plant volatiles in contained greenhouse settings

A laboratory high‐throughput glass chamber using dynamic headspace TD‐GC/MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium‐related abiotic stress

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